IP3(Inositol Triphosphate) ELISA Kit
Ref. EK-ELK8148
48T | 334,00 € | ||
96T | 471,00 € |
Información del producto
- InsP3; Inositol 1,4,5-Trisphosphate; Triphosphoinositol
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with IP3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to IP3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of IP3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Propiedades químicas
Consulta técnica sobre: EK-ELK8148 IP3(Inositol Triphosphate) ELISA Kit
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