Zebrafish GnRH(Gonadotropin Releasing Hormone) ELISA Kit

Ref. EK-ELK9172

Información del producto

• Nombre: Zebrafish GnRH(Gonadotropin Releasing Hormone) ELISA Kit
• Sinónimos: GNRH1; GRH; LNRH; LHRH; LH-RH I; Luliberin; Luteinizing-Hormone Releasing Hormone; Progonadoliberin-1; Gonadoliberin I; Gonadorelin; GnRH-associated peptide 1

Descripción

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Zebrafish GnRH protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Zebrafish GnRH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Zebrafish GnRH in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marca: ELK Biotechnology