Rat TRACP-5b(TartrateResistant Acid Phosphatase 5b) ELISA Kit

Ref. EK-ELK9632

Información del producto

• Nombre: Rat TRACP-5b(TartrateResistant Acid Phosphatase 5b) ELISA Kit
• Sinónimos: ACP5;TRAP5b;SPENCDI

Descripción

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat TRACP-5b. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat TRACP-5b. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat TRACP-5b, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat TRACP-5b in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marca: ELK Biotechnology