Cattle THP(Tamm-Horsfall Glycoprotein) ELISA Kit

Ref. EK-ELK9723

Información del producto

• Nombre: Cattle THP(Tamm-Horsfall Glycoprotein) ELISA Kit
• Sinónimos: THP; Uromucoid,Tamm-Horsfall Glycoprotein; Tamm-Horsfall urinary glycoprotein; Uromodulin; UMOD

Descripción

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle THP. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle THP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle THP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle THP in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marca: ELK Biotechnology