Plasmid Isolation Kit from Bacterial cells (Teaching)

Ref. SR-27725

Información del producto

• Nombre: Plasmid Isolation Kit from Bacterial cells (Teaching)

Descripción

The overall goal is to isolate extra-chromosomal, plasmid DNA from bacterial cells that contain the plasmid. The cells are first re-suspended in a suitable buffer (that minimizes nuclease activity) and ruptured to release the cellular contents. Subsequently, the plasmid DNA and RNA are separated from genomic DNA. Finally, by decreasing the “effective concentration” of water, the plasmid DNA is precipitated through sheer desolvation effects. If desired, the contaminating RNA can be removed by digestion with RNase A.

• Marca: Sisco Research Laboratories