CAS 61970-00-1

Luciférase de luciole

Description :

Luciférase de luciole est une enzyme dérivée des lucioles, spécifiquement de l'espèce Photinus pyralis, et est connue pour son rôle dans la bioluminescence. Cette enzyme catalyse l'oxydation de la luciférine, un composé émetteur de lumière, en présence d'oxygène, ce qui entraîne la production de lumière. Luciférase de luciole se caractérise par sa haute spécificité pour la luciférine et sa capacité à produire de la lumière dans une gamme de conditions, ce qui en fait un outil précieux en biologie moléculaire et en biochimie. Elle est couramment utilisée dans des essais bioluminescents, des études d'expression génique et comme gène rapporteur dans diverses applications en raison de sa sensibilité et de sa facilité de détection. L'enzyme fonctionne de manière optimale à un pH et une température physiologiques, et son activité peut être influencée par des facteurs tels que la force ionique et la présence de cofacteurs. Le numéro CAS 61970-00-1 identifie de manière unique cette enzyme dans les bases de données chimiques, facilitant la recherche et l'application dans les études scientifiques. Dans l'ensemble, Luciférase de luciole est un composant crucial dans diverses applications biotechnologiques, tirant parti de ses propriétés luminescentes naturelles.

Formule : Unspecified

Synonymes :

• Beetle luciferase
• Click beetle luciferase
• E.C. 1.13.12.7
• Ec 1.13.12.7
• Firefly luciferase
• Firefly luciferin luciferase
• Luciferasa (Luciferina De Luciernaga)
• Luciferase
• Luciferase (Leuchtkaferluciferin)
• Luciferase (Luciferine De Luciole)
• Luciferase (Macrolampis luciferin)
• Luciferase (firefly)
• Luciferase-LM plus
• Luciola mingrelica luciferase
• Macrolampis luciferase
• Photinus luciferin 4-monooxygenase (adenosine triphosphate-hydrolyzing)
• Photinus pyralis luciferase
• Photinus scintillans luciferase
• Promega luciferase
• Pyrocoelia pygidialis luciferase
• Pyrophorus plagiophthalamus luciferase
• QuantiLum Recombinant Luciferase
• RFLuc
• Ultra-Glo
• Ultra-Glo luciferase
• Luciferase, Firefly, Recomb.
• Luciferase (firefly luciferin)

Luciferase, firefly

CAS : 61970-00-1

Luciferase, firefly is a reporter gene in cellular imaging studies to quantify target gene expression levels and measure intracellular ATP content,

Degré de pureté : 98%

Couleur et forme : Solid

Masse moléculaire : N/A

Ref: TM-TP1774

X-Shining™ Luciferase, lyophilised

CAS : 61970-00-1

Supplied as lyophilisate containing 1% w/w X-Shining™ Luciferase. The improved, thermostable X-Shining™ Luciferase is suitable for any luciferin-luciferase-based assay using D-luciferin (dLuc) or synthetic pro-luciferins (caged luciferins). Examples of its typical applications include ATP tests in hygiene monitoring, ATP tests in drug screenings and bacteria identification in microbial assays. The termostable luciferase X-Shining™ has been optimised by genetic engineering for strongly increased thermostability and storage stability. In temperature stress tests, the enzyme survives temperatures of 60°C for over an hour, whereas a wild type luciferase from firefly is inactivated after only a few minutes. The extraordinary stability makes it user-friendly and eliminates some of the main disadvantages and limitations of the commonly used wild type luciferase. X-Shining™ Luciferase is supplied as a lyophilisate (1% w/w X-Shining™ Luciferase) or as aqueous solution with glycerol (L-8093), and it may be stored for months at room temperature without significant loss of function. Find out more about our innovative X-Shining range here X-Shining | Innovation | Cymit Quimica Carbosynth.

Ref: 3D-L-8095

Luciferase - from Photinus pyralis (firefly)

CAS : 61970-00-1

Luciferase enzyme from Photinus pyralis (firefly), which catalyzes the oxidation of firefly luciferin. This reaction depends on the presence of oxygen and ATP and causes the bioluminescence seen in fireflies

Degré de pureté : (Gel Electrophoresis) Min. 98%

Couleur et forme : Powder

Ref: 3D-FL162293

X-Shining™ Luciferase, 10 mg/mL aqueous solution with glycerol

CAS : 61970-00-1

The improved, thermostable X-ShiningTM Luciferase is suitable for any luciferin-luciferase-based assay using D-luciferin (dLuc) or synthetic pro-luciferins (caged luciferins). Examples of its typical applications include ATP tests in hygiene monitoring, ATP tests in drug screenings and bacteria identification in microbial assays. The thermostable luciferase X-ShiningTM has been optimised by genetic engineering for strongly increased thermostability and storage stability. In temperature stress tests, the enzyme survives temperatures of 60 °C for over an hour, whereas a wild type luciferase from firefly is inactivated after only a few minutes. The extraordinary stability makes it user-friendly and eliminates some of the main disadvantages and limitations of the commonly used wild type luciferase. X-ShiningTM Luciferase is supplied as aqueous solution with glycerol or as a lyophilisate (L-8095), and it may be stored for months at room temperature without significant loss of function. Find out more about our innovative X-Shining range here X-Shining | Innovation | Cymit Quimica Carbosynth.

Degré de pureté : (Sds-Page) Min. 90%

Ref: 3D-L-8093

econoLuciferase, buffered aqueous solution

CAS : 61970-00-1

Cymit Quimica’s econoLuciferase™ (econoLuc Cymit Quimica Cat. No. L-8090) is a recombinant luciferase from the firefly Luciola lateralis that has been expressed as a luciferase-GFP fusion protein in E. coli.The luciferase enzyme has been optimized for increased thermo-stability by genetic modification to be stable for one hour at 37°C and up to two days at room temperature.Stabilized econoLuciferase™ thus overcomes the disadvantages and limitations of wild-type Luciferase, notably its short active life, outperforming enzyme from the Photinus pyralis firefly for both performance and stability.This optimized luciferase is the perfect enzyme for ATP detection assays in hygiene control, microbial tests using caged luciferins and it is the luciferase of choice for biochemical tests measuring ATP consumption and production in diverse enzymatic reactions.The product L-8090 is available on a large scale and is intended for use in the chemical, diagnostic, pharmaceutical and related industries.

Degré de pureté : (Spec. Activity. U/Mg) Min. 5 X 10^8

Ref: 3D-L-8090

X-Shining™ Luciferase, lyophilised

CAS : 61970-00-1

Supplied as lyophilisate containing 1% w/w X-Shining™ Luciferase. The improved, thermostable X-Shining™ Luciferase is suitable for any luciferin-luciferase-based assay using D-luciferin (dLuc) or synthetic pro-luciferins (caged luciferins). Examples of its typical applications include ATP tests in hygiene monitoring, ATP tests in drug screenings and bacteria identification in microbial assays. The termostable luciferase X-Shining™ has been optimised by genetic engineering for strongly increased thermostability and storage stability. In temperature stress tests, the enzyme survives temperatures of 60°C for over an hour, whereas a wild type luciferase from firefly is inactivated after only a few minutes. The extraordinary stability makes it user-friendly and eliminates some of the main disadvantages and limitations of the commonly used wild type luciferase. X-Shining™ Luciferase is supplied as a lyophilisate (1% w/w X-Shining™ Luciferase) or as aqueous solution with glycerol (BX174908), and it may be stored for months at room temperature without significant loss of function. Find out more about our innovative X-Shining range here X-Shining | Innovation | Cymit Quimica Carbosynth.

Degré de pureté : (Spec. Activity. U/Mg) Min. 10^7

Couleur et forme : Powder

Ref: 3D-BX180145