Substrats fluorogènes

<p>4-Methylumbelliferyl-beta-D-GlcNAc-6-phosphocholine is a fluorogenic substrate for phosphodiesterase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl-beta-D-GlcNAc-6-phosphocholineas a substrate for measuring the phosphodiesterase activity is used for Niemann-Pick disease diagnostic assays. It is also used for sphingomyelin metabolism disorder screening.</p>

<p>This botanical is a download for the Floral Database.</p>

<p>6,8-Difluoro-4-methylumbelliferyl 2-acetamido-3-O-(b-D-galactopyranosyl)-a-D-galactopyranoside is a chemiluminescent substrate that reacts with oxygen in the presence of luciferase to produce light. This product is an excellent replacement for luminol, especially in applications where aqueous solvents are not suitable. 6,8-Difluoro-4-methylumbelliferyl 2-acetamido-3-O-(b-D-galactopyranosyl)-a-D-galactopyranoside has been extensively tested and shown to be nonhazardous in a variety of different culture media. It is also nonhazardous to the environment, as it can be easily degraded by microorganisms.</p>

<p>4-Methylumbelliferyl 6-O-(N-acetylneuraminyl)-b-D-glucopyranoside is a fluorescent substrate for the enzyme β-D-galactosidase. It is used in the detection of β-D-galactosidase activity in the diagnosis of various diseases, including celiac disease and AIDS. 4-Methylumbelliferyl 6-O-(N-acetylneuraminyl)-b-D-glucopyranoside can also be used as a chromogenic or fluorogenic substrate for chemiluminescent detection of β-D-galactosidase activity. The conjugate with horseradish peroxidase can be used to detect hydrogen peroxide in water samples, such as drinking water, wastewater, and surface waters. 4MUG has been shown to have high purity and is CAS No. 78761–35–4.</p>

<p>4-Methylumbelliferyl-a-D-mannopyranoside-6-phosphoethanolamine is a synthetic fluorogenic substrate that can be used in the detection of phosphatases. This substrate is a chromogenic and fluorogenic substrate that reacts when it is cleaved by enzymes. It has been shown to work with a number of different enzyme types, including esterases, phosphatases, and proteases. 4-Methylumbelliferyl-a-D-mannopyranoside-6-phosphoethanolamine has demonstrated high quality for use in bioluminescence, food testing, and environmental testing.4Methylumbelliferyl - a - D - mannopyranoside - 6 - phosphoethanolamine has been assigned CAS No. 81771-07-2.</p>

<p>4-Trifluoromethylumbelliferyl b-D-xylopyranoside (4TUF) is a fluorescent probe that can be used to monitor the activity of protein kinases. The fluorescence intensity of 4TUF changes in response to the phosphorylation state of the target protein. This probe can be used in computational and experimental investigations of protein kinase signalling pathways, as well as for optimizing drug design.</p>

<p>4-Methylumbelliferyl beta-D-apiofuranoside is a fluorescent substrate that is used in the detection of enzyme activity. It is a yellow crystalline powder with a melting point of 132°C and an optical rotation of +27°. 4-Methylumbelliferyl beta-D-apiofuranoside can be used as a conjugate for fluorescence, chemiluminescence, or bioluminescence detection of enzymes such as β-galactosidase, phosphatases, esterases, and peroxidases. It is also used to stain cells and tissues for microscopic analysis. This product has been tested for its effects on the environment and found to have little potential for harm to the environment. 4-Methylumbelliferyl beta-D-apiofuranoside has been shown to be high purity with no significant impurities detected in testing. The chemical has shown high quality characteristics in</p>

<p>Aldol® 355 fluorescence enhancer is an enhancer of fluorescence signals of the Aldol® fluorogenic substrates. Aldol® 355 fluorescence enhancer allows concentrations of fluorogenic Aldol® substrates in media to reduce up to 6 times. Aldol® 355 fluorescence enhancer can be combined with Aldol® substrates, releasing water-insoluble dyes upon enzymatic cleavage, such as Aldol® 470 range, Aldol® 495 range, Aldol® 518 range, and Aldol®515 range of fluorogenic enzyme substrates.</p>

<p>4-Trifluoromethylumbelliferyl b-D-glucopyranoside is a fluorogenic substrate for beta-glucosidase. Upon hydrolysis, the fluorogenic moiety (blueish) 4-trifluoromethylumbelliferone (also known as TFMU) is released. 4-Trifluoromethylumbelliferyl b-D-glucopyranoside is commonly used in bacterial detection and in Gaucher disease diagnosis.</p>

<p>4-Methylumbelliferyl dodecanoate is a fluorogenic substrate for lipase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl dodecanoateas a substrate for measuring the lipase activity is used for detecting pancreatic insufficiency in diagnosis assays as well as the screening of lipase deficiency.</p>

<p>A substrate used for the diagnosis of Sanfilippo disease C (MPS III C).</p>

<p>4-Methylumbelliferyl alpha-D-glucosaminide is a fluorogenic substrate for alpha-N-acetylglucosaminidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl alpha-D-glucosaminideas a substrate for measuring the alpha-N-acetylglucosaminidase activity is used for Sanfilippo syndrome B and Mucopolysaccharidosis IIIB (MPS IIIB) diagnosis.</p>

<p>L-Alanine-7-amido-4-methylcoumarin hydrochloride is a synthetic peptide substrate used as a fluorogenic substrate for alanine aminopeptidase.</p>

<p>4-Methylumbelliferyl 2-acetamido-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranoside is a fluorogenic substrate for alpha-N-acetylgalactosaminidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-acetamido-3-O-(beta-D-galactopyranosyl)-alpha-D-galactopyranosideas a substrate for measuring the alpha-N-acetylgalactosaminidase activity is used for Fabry disease diagnosis and Schindler disease screening.</p>

<p>(+)-Biotin 4-amidobenzoic acid sodium salt, often referred as (+)-biotin PABA, is a fluorogenic substrate used to measure biotinidase activity, especially in newborn screening programs.</p>

<p>PAIRED PRODUCT AVAILABLE: alpha-L-iduronidase Enzyme, FI179383.<br>4MU-alpha-L-idopyranosiduronic acid is a fluorogenic substrate for alpha-L-iduronidase. Active alpha-L-iduronidase cleaves the MU moiety from the synthetic substrate, which results in release of a fluorescent signal (Ex 360 nm / Em 455 nm). The reaction is stopped by the addition of sodium carbonate. 4MU-alpha-L-idopyranosiduronic acid is used in alpha-L-iduronidase assays in the context of biochemical characterisation of samples from patients affected by lysosomal storage disease mucopolysaccharidosis I (MPS I).</p>

<p>4-Methylumbelliferyl palmitate, commonly names as 4-MUP, is a fluorescent probe that binds to lipases, enzymes responsible to assist on the hydrolysis of fats. In diagnosis, 4-MUP is used for screening of lysosomal storage diseases (LSD) caused by a deficiency of a single enzyme involved in the metabolism of glycoproteins, lipids or mucopolysaccharides.</p>

<p>Resorufin b-D-glucuronide sodium salt is used as a fluorogenic substrate for beta-glucuronidase (GUS) to study drug metabolism.</p>

<p>4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-galactopyranoside-4-sulfate sodium salt is a fluorogenic substrate for N-acetylgalactosamine-4-sulfatase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-acetamido-2-deoxy-beta-D-galactopyranoside-4-sulfate sodium saltas a substrate for measuring the N-acetylgalactosamine-4-sulfatase activity is used for diagnosis of Mucopolysaccharidosis VI (MPS VI) (a rare lysosomal storage disorder) and Maroteaux-Lamy syndrome screening.</p>

<p>Fluorochrome for calibration of fluorimeters.  Used with corresponding fluorogenic substrates for the diagnosis of Krabbe - and Niemann-Pick A/B disease.</p>

<p>Resorufin b-D-xylobioside is a fluorogenic substrate for xylanases useful for xylanase activity assays.</p>

<p>Methylumbelliferyl oleate is a MOSCERDAM® substrate targeting the lipase enzyme</p>

<p>PAIRED PRODUCT AVAILABLE: alpha-L-iduronidase Enzyme, FI1793834MU-alpha-L-iduronide-2-sulphate (M2) is a fluorogenic substrate of iduronate-2-sulphatase (IDS), used in a 2-step assay coupled with alpha-L-iduronidase. The assay is used in biochemical testing for Hunter syndrome (mucopolysaccharidosis II / MPS II). In a first step, IDS from biological samples cleaves the sulphate group from M2. In a second step, exogeneous alpha-L-iduronidase which cleaves the 4-MU moiety is added to the reaction, resulting in release of a fluorescent signal (Ex 360 nm / Em 455 nm). The reaction is stopped by the addition of sodium carbonate.</p>

<p>4-Methylumbelliferyl pyrophosphate diester disodium salt is a fluorogenic substrate for pyrophosphatases or diphosphatases. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl pyrophosphate diester disodium saltas a substrate for measuring the pyrophosphatases or diphosphatases activity is used for monitoring and diagnosis of pyrophosphatase deficiency and bone metabolism disorder.</p>

<p>Fluorogenic substrate for beta-galactosidase</p>

<p>4-Methylumbelliferyl-2-O-(alpha-L-fucopyranosyl)-alpha-L-fucopyranoside is a fluorogenic substrate for alpha-L-fucosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl-2-O-(alpha-L-fucopyranosyl)-alpha-L-fucopyranosideas a substrate for measuring the alpha-L-fucosidase activity is used for fucosidosis diagnosis.</p>

<p>FLsharp™ - Phosphate, disodium salt is a fluorogenic water-soluble substrate for phosphatase enzymes. Its chemical name is 6-Chloro-2-[5-chloro-2-(phosphonooxy)phenyl]-4(1H)-quinazolinone disodium salt and was developed by Molecular Probes in the 1990s. After cleavage of the substrate by phosphatase activity, an insoluble product is formed that is highly fluorescent in the solid state. Upon UV irradiation this very stable fluorophore emits green light in the 530 nm range for an extended period of time. FLsharp™ is a unique substrate - optimized for the detection of phosphatase activity in solid matrices such as agar media, tissue sections or microscopic preparations. FLsharp™ - Phosphate, disodium salt is used in microbiology as a substrate for bacterial phosphatases.</p>

<p>Fluorescein mono-b-D-galactopyranoside or FMG is a fluorogenic substrate for beta-galactosidase. The fluorophore fluorescein is released after enzymatic cleavage, exhibiting a greenish fluorescent. This substrate is particularly valuable in gene expression studies (e.g., lacZ reporter assays) and as a probe for cell-based assays for real-time monitoring.</p>

<p>Acetyl-L-alanine 7-amido-4-methylcoumarin is a fluorogenic substrate for aminopeptidase. The enzyme cleaves the N-terminal amino acids, yielding a blue fluorescent solution.</p>

<p>Fluorogenic substrate for esterase yielding a blue fluorescent solution upon cleavage.</p>

<p>Fluorogenic cellulose analogue</p>

<p>L-Histidine 7-amido-4-methylcoumarin hydrochloride is a synthetic peptide substrate used as a fluorogenic substrate for histidine aminopeptidase.</p>

<p>4-Methylumbelliferyl 6-deoxy-2,3,4,6-tetra-O-acetyl-6-thio-beta-D-glucopyranoside is a fluorogenic substrate for beta-glucosidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 6-deoxy-2,3,4,6-tetra-O-acetyl-6-thio-beta-D-glucopyranosideas a substrate for measuring the beta-glucosidase activity is used for Gaucher disease diagnosis.</p>

<p>A fluorogenic MOSCERDAM® substrate targeting cellulases</p>

<p>4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate potassium salt is a fluorogenic substrate for beta-N-acetylglucosaminidase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate potassium saltas a substrate for measuring the beta-N-acetylglucosaminidase activity is used for diagnosis of Mucopolysaccharidosis IIIB (MPS IIIB) (a rare lysosomal storage disorder) and Sanfilippo B syndrome screening.</p>

<p>N-alpha-CBZ-L-Arginine 7-amido-4-methylcoumarin hydrochloride is a Ffluorogenic substrate used in protease activity assays.</p>

<p>4-Methylumbelliferyl alpha-D-mannopyranoside 6-sulfate sodium salt is a fluorogenic substrate for sulfatase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl alpha-D-mannopyranoside 6-sulfate sodium saltas a substrate for measuring the sulfatase activity is used for Mucopolysaccharidosis (MPS) screening and other lysosomal storage disorder diagnosis.</p>

<p>Glycyl-glycine 7-amido-4-methylcoumarin hydrochloride is a synthetic peptide substrate used as a fluorogenic substrate for aminopeptidase.</p>

<p>4-MU-Gal-6S (M4A) is a fluorogenic substrate of galactose-6-sulfate sulfatase (GALNS), used for the diagnosis of Mucopolysaccharidosis IV type A (MPS IV A). MPS IV, also known as Morquio syndrome, is a rare metabolic disorder in which the human body cannot process glycosaminoglycans (GAGs). MPS IV A is caused by the deficiency of the enzyme N-acetyl-galactosamine-6-sulfatase resulting in accumulation of keratan and chondroitin sulfate in tissues. M4A is used as the GALNS substrate in the anti-lysosomal enzyme antibody assay for the treatments of lysosomal storage disorders (LSDs) with enzyme replacement therapies (ERTs).</p>

<p>4-Methylumbelliferyl lignocerate is a fluorogenic substrate for lipase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl lignocerateas a substrate for measuring the lipase activity is used for X-linked adrenoleukodystrophy diagnosis and peroxisomal disorder screening.</p>

<p>The DDAO molecule [7-hydroxy-9H-(1, 3-dichloro-9, 9 dimethylacridin-2-one] is used as a stain and a dye in molecular biology, due to its ability to bind intracellular and extracellular DNA. DDAO galactoside is a fluorogenic substrate for galactosidase.</p>

<p>Sensitive fluorogenic substrate for fluorometric and UV-spectrophotometric assays of phosphatases. This may also be used for in-gel assays</p>

<p>Monofluoresceinated mannitol is a fluorescently labeled form of mannitol. It is used as a tracer for metabolism studies.</p>

<p>A fluorogenic substrate for detection of enzymatic activity of the SARS-CoV-2 Mpro protease. The fluorophore 7-Amino-4-methylcoumarin (AMC) is linked to the tetrapeptide via a peptide bond at the C-terminal of the glutamine residue. Following the hydrolytic activity of the Mpro protease, the fluorogenic AMC group get released from the substrate, generating a fluorescent signal (Ex 380 nm / Em 455 nm).</p>

<p>L-Lysyl-L-alanine 7-amido-4-methylcoumarin dihydrochloride is a fluorogenic peptide substrate for aminopeptidase. This AMC peptide substrate is normally used to rapidly profile the N-terminal specificity of proteases.</p>

<p>4-Methylumbelliferyl 2-deoxy-2-sulfamino-beta-D-glucopyranoside sodium salt is a fluorogenic substrate for N-sulfoglucosamine sulfohydrolase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-deoxy-2-sulfamino-beta-D-glucopyranoside sodium saltas a substrate for measuring the N-sulfoglucosamine sulfohydrolase activity is used for screening of Mucopolysaccharidosis IIIA (MPS IIIA) and diagnosis of Sanfilippo A syndrome.</p>

<p>4-Methylumbelliferyl 2-acetamido-2-deoxy-alpha-D-glucopyranoside-6-sulfate potassium is a fluorogenic substrate for N-acetylglucosamine-6-sulfatase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl 2-acetamido-2-deoxy-alpha-D-glucopyranoside-6-sulfate potassiumas a substrate for measuring the N-acetylglucosamine-6-sulfatase activity is used for screening of Mucopolysaccharidosis IIID (MPS IIID) (a rare lysosomal storage disorder) and Sanfilippo D syndrome diagnosis.</p>

<p>5-Dodecanoylaminofluorescein di-b-D-galactopyranoside, commonly known as C12FDG, is a fluorogenic substrate for β-galactosidase. Fluorescein is released upon enzymatic cleavage by β-galactosidase. The intensity of its fluorescence is utilised to quantify β-galactosidase activity. C12FDG has been shown to be useful in identifying senescent cells that are associated with aging and cancer. It has also been used as a model system in some studies for studying disease activity, intracellular targets, and biological sample storage. It is also used to identify cancer tissues and radiation damage in the form of senescent cells. The chemical structure of 5DAFG is similar to that of the natural sugar galactose. 5DAFG binds to the plasma membrane of cells due to its lipophilic properties and then enters the cell through transport proteins on the cell surface called ATP binding cassette transporters.</p>

<p>Fluorogenic substrate for beta-galactosidase</p>

<p>4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt is a high-quality fluorogenic substrate specifically designed for the detection and quantification of various enzymes, including beta-glucosidases and sulfatases. Upon hydrolysis by the target enzyme, this substrate releases the highly fluorescent 4-methylumbelliferone, allowing for real-time monitoring and accurate measurement of enzyme activities in biochemical assays, diagnostic tests, and research applications. 4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt has excellent solubility in water, ensuring a consistent and reliable performance in different experimental conditions. This versatile substrate is an essential tool for researchers and industry professionals who require a sensitive and reproducible detection method for enzymes of interest.</p>