Électrophorèse
Dans cette section, vous trouverez tous les réactifs et matériaux nécessaires pour réaliser l'électrophorèse, une technique de laboratoire essentielle utilisée pour séparer les protéines en fonction de leur masse moléculaire et de leur charge. Cette technique est cruciale pour analyser la composition et la pureté des échantillons de protéines, ainsi que pour étudier les acides nucléiques et autres biomolécules. Notre sélection comprend des tampons d'électrophorèse, des gels, des colorants et des marqueurs de haute qualité, ainsi que des appareils et accessoires conçus pour garantir une séparation précise et efficace. Ces produits sont indispensables pour la recherche en biologie moléculaire, biochimie et génétique, fournissant des résultats fiables pour la caractérisation des protéines, l'analyse de l'expression génique et d'autres applications. Chez CymitQuimica, nous fournissons tout ce dont vous avez besoin pour réaliser l'électrophorèse, soutenant vos recherches avec précision et cohérence.
Sous-catégories appartenant à la catégorie "Électrophorèse"
298 produits trouvés pour "Électrophorèse"
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Glycerol [for Electrophoresis]
CAS :Formule :C3H8O3Degré de pureté :>99.0%(GC)Couleur et forme :Colorless clear liquidMasse moléculaire :92.093,3'-Diaminobenzidine [for Biochemical Research]
CAS :Formule :C12H14N4Degré de pureté :>97.0%(T)Couleur et forme :Light yellow to Brown powder to crystalMasse moléculaire :214.27Acrylamide Monomer [for Electrophoresis]
CAS :Formule :C3H5NODegré de pureté :>98.0%(T)Couleur et forme :White powder to crystalMasse moléculaire :71.08Fast Green FCF [for Biochemical Research]
CAS :Formule :C37H34N2Na2O10S3Degré de pureté :>85.0%(E)(HPLC)Couleur et forme :Amber to Dark purple to Black powder toMasse moléculaire :808.843,3'-Diethyloxadicarbocyanine Iodide
CAS :Formule :C23H23IN2O2Degré de pureté :>90.0%(HPLC)Couleur et forme :Blue to Dark blue to Dark purple powder to crystalMasse moléculaire :486.35TCEP Hydrochloride [for Electrophoresis]
CAS :Formule :C9H15O6P·HClDegré de pureté :>98.0%(T)Couleur et forme :White to Light yellow powder to crystalMasse moléculaire :286.64Western Blot Stripping Buffer [for Biochemical Research]
Couleur et forme :Colorless to Almost colorlessclear liquid1-Thioglycerol [for Electrophoresis]
CAS :Formule :C3H8O2SDegré de pureté :>95.0%(GC)(T)Couleur et forme :Colorless to Almost colorless clear liquidMasse moléculaire :108.16Acrylamide/Bis-acrylamide, 37.5:1, 30% solution
Couleur et forme :Clear, colourless liquidMasse moléculaire :-Bovine Serum Albumin, pH 5.2
CAS :Degré de pureté :≥ 96.0%Couleur et forme :Off-white to light-yellow or green powder or flakesMasse moléculaire :-Acrylamide 4X solution
CAS :Formule :C3H5NOCouleur et forme :Clear, colourless liquidMasse moléculaire :71.08Sodium dodecyl sulfate, 20% solution in water
CAS :Formule :C12H25NaO4SCouleur et forme :Clear, colourless liquidMasse moléculaire :288.38Hydrochloric acid 1%
CAS :Produit contrôléFormule :HClCouleur et forme :Clear, colourless liquidMasse moléculaire :36.46Bovine Serum Albumin, fatty acid free
CAS :Degré de pureté :≥ 98%Couleur et forme :Off-white to light-yellow or green powder or flakesMasse moléculaire :-Starch (Smithies); for electrophoresis
CAS :Formule :(C6H10O5)nCouleur et forme :White powderMasse moléculaire :(162.14)nBovine Serum Albumin, GlenCell™, suitable for cell culture
CAS :Degré de pureté :≥ 98.0%Couleur et forme :Off-white to light-yellow or green powder or flakesMasse moléculaire :-Methylene Blue solution, 1% in water
CAS :Formule :C16H18ClN3SCouleur et forme :Blue to very dark blue liquidMasse moléculaire :319.86Hydrochloric acid, 4% (w/v) in water
CAS :Produit contrôléFormule :HClCouleur et forme :Clear, colourless solutionMasse moléculaire :36.46DL-Dithiothreitol, 98%
CAS :Formule :C4H10O2S2Degré de pureté :(Titration) 98.0 - 102.0 %Couleur et forme :White or almost white powderMasse moléculaire :154.25Laemmli Sample Buffer 4X (without 2-Mercaptoethanol)
Couleur et forme :Clear, blue to very dark blue liquidMasse moléculaire :-Sodium hydroxide, 2.0M solution
CAS :Formule :NaOHCouleur et forme :Clear, colourless liquidMasse moléculaire :40.00Sodium hydroxide pellets, EP
CAS :Formule :NaOHDegré de pureté :(Titration) 97.0 - 100.5 %Couleur et forme :White pelletsMasse moléculaire :40.00N,N''-Methylene-bis-acrylamide 2X solution
CAS :Formule :C7H10N2O2Couleur et forme :Clear, colourless liquidMasse moléculaire :154.17Sodium thiosulfate solution
CAS :Formule :Na2O3S2Couleur et forme :Clear, colourless liquidMasse moléculaire :158.10TRIS-Glycine Buffer 10X, GlenBiol™, suitable for molecular biology
Couleur et forme :Clear, colourless liquidMasse moléculaire :-Silver nitrate, 99.9%+ AR grade
CAS :Formule :AgNO3Degré de pureté :≥ 99.9%Couleur et forme :White crystals or crystalline powderMasse moléculaire :169.88Acrylamide/Bis-acrylamide, 37.5:1, 40% solution
Couleur et forme :Clear, colourless liquidMasse moléculaire :-Bovine Serum Albumin, 98% by electrophoresis
CAS :Degré de pureté :≥ 98%Couleur et forme :Off-white to pale yellow or green powder or flakesMasse moléculaire :-TRIS-Glycine-SDS Buffer 10X, GlenBiol™, suitable for molecular biology
Couleur et forme :Clear, colourless liquidMasse moléculaire :-TRIS-Tricine-SDS running buffer, 10X solution
Couleur et forme :Clear, colourless liquidMasse moléculaire :-Acrylamide/Bis-acrylamide, 19:1, 30% solution
Couleur et forme :Clear, colourless liquidMasse moléculaire :-Potassium phosphate monobasic, 1M solution
CAS :Formule :H2KO4PCouleur et forme :Clear, colourless liquidMasse moléculaire :136.09Zinc sulfate heptahydrate
CAS :Formule :ZnSO4·7H2ODegré de pureté :≥ 98% (ZnSO4 · 7H2O)Couleur et forme :White crystalline powder or colourless crystalsMasse moléculaire :287.54Potassium carbonate, anhydrous
CAS :Formule :CK2O3Degré de pureté :≥ 99.5%Couleur et forme :White powderMasse moléculaire :138.21Bromophenol Blue
CAS :<p>Bromophenol Blue, an acid phthalein dye, is utilized both as a tracking dye for electrophoresis and as a pH indicator with a transition range of pH 3 to 4.6.</p>Formule :C19H10Br4O5SDegré de pureté :99.59%Couleur et forme :Light Pink To Purple Crystalline PowderMasse moléculaire :669.96Methylene Blue solution, 0.5% in water
CAS :Formule :C16H18ClN3SDegré de pureté :0.5g/100ml distilled waterCouleur et forme :Blue to dark blue liquidMasse moléculaire :319.86Sodium hydroxide, 1.0M solution
CAS :Formule :NaOHCouleur et forme :Clear, colourless liquidMasse moléculaire :40.00Acrylamide/Bis-acrylamide, 19:1, 40% solution
Couleur et forme :Clear, colourless liquidMasse moléculaire :-Agarose, low EEO, GlenBiol™, suitable for molecular biology
CAS :Couleur et forme :White or off-white powderMasse moléculaire :-Sodium chloride
CAS :Formule :NaClDegré de pureté :≥ 99.9% (dried basis)Couleur et forme :White crystalline powderMasse moléculaire :58.44Methanol, for HPLC, gradient grade, 99.9%
CAS :Formule :CH3OHDegré de pureté :≥ 99.9%Couleur et forme :Clear, colourless liquidMasse moléculaire :32.04Bovine Serum Albumin, 99%
CAS :Degré de pureté :≥ 99% (protein)Couleur et forme :Off-white to light-yellow or green powder or flakesMasse moléculaire :-Sodium dodecyl sulfate, 10% solution in water
CAS :Formule :C12H25NaO4SCouleur et forme :Clear, colourless liquidMasse moléculaire :288.3810X Tris-Glycine pH-8.3 Tank Buffer for molecular biology
<p>The 10X Tris-Glycine Buffer, with a pH of 8.3, is designed specifically for use in native PAGE gel electrophoresis. This buffer offers great convenience, as it can be diluted with distilled water or another suitable solvent according to the specific application prior to electrophoresis. Additionally, it is useful for the transfer of protein samples onto nitrocellulose membranes during western blotting. To prevent the gel from swelling during the transfer process and to enhance the binding of protein samples to nitrocellulose, the inclusion of methanol is essential.</p>Couleur et forme :Clear, Colourless, LiquidTris(Hydroxymethyl) Aminomethane Acetate for molecular biology (Tris Acetate Buffer), 99%
CAS :Formule :C4H11NO3·C2H4O2Degré de pureté :min. 99%Couleur et forme :White, Crystalline powder, Clear, ColourlessMasse moléculaire :181.19N,N-Methylene Bisacrylamide 3x cryst. for molecular biology, 99.5%
CAS :Formule :C7H10N2O2Degré de pureté :min. 99.5%Couleur et forme :White, Crystalline powder, Clear, ColourlessMasse moléculaire :154.1710X Phosphate Buffered Saline Tween-20 (PBST) for molecular biology
Couleur et forme :Clear, Colourless with yellow tinted, Liquid1M Tris Hydrochloride Buffer (1M Tris HCl) pH-7.2 for molecular biology
Couleur et forme :Clear, Colourless, LiquidBrilliant Blue R-250 for molecular biology
CAS :Formule :C45H44N3NaO7S2Couleur et forme :Dark blue with reddish tinge, PowderMasse moléculaire :825.981X Phosphate Buffered Saline (PBS) for molecular biology
The pH of the buffer is typically maintained within a range of 7.4 ± 0.1, closely resembling the physiological pH found in many biological systems.Couleur et forme :Clear, Colourless, Liquid10X MOPS Buffer for molecular biology
MOPS, or morpholino propanesulfonic acid, is a structural analog of MES, the ethanesulfonic acid. With a pKa value of 6.80-7.20, MOPS serves as an excellent buffer for many biological systems at near-neutral pH. It is extensively used as a buffering agent in molecular biology and biochemistry and has been tested and recommended for use in polyacrylamide gel electrophoresis. Additionally, MOPS is applicable in various bioanalytical methods, including isoelectric focusing, protein assays, and X-ray crystallographic studies. Furthermore, it can be used as an electrophoresis buffer for agarose gel electrophoresis of RNA.Couleur et forme :Clear, Colourless, Liquid20X SSC Buffer pH 6.9-7.1 for molecular biology
The Saline-Sodium Citrate Hybridization Buffer [20X] is a key component in various nucleic acid techniques, ensuring optimal stringency during washing steps in procedures such as Southern blotting, in situ hybridization, DNA microarrays, and Northern blotting. When utilized as 20X SSC, it effectively prevents agarose gels from drying out during vacuum transfers, preserving sample integrity. This solution is specifically formulated to support the diverse needs of end users in nucleic acid hybridizations and blot transfer applications, making it an invaluable resource in molecular biology workflows.Couleur et forme :Clear, Colourless, SolutionLithium Lauryl Sulphate extrapure, 99%
CAS :Formule :C12H25SO4LiDegré de pureté :min. 99%Couleur et forme :White, Crystalline powder, Clear, ColourlessMasse moléculaire :272.30Albumin Bovine Solution 10% Diluent Solution in PBS (BSA 10% Diluent/Blocking Solution in PBS)
CAS :Couleur et forme :Clear, Pale yellow, Liquid1M Tris Hydrochloride Buffer (1M Tris HCl) pH-8.0 for molecular biology
Couleur et forme :Clear, Colourless, LiquidN,N'-Methylene Bisacrylamide 2% Aq. Solution for molecular biology
CAS :Formule :C7H10N2O2Couleur et forme :Clear, Colourless, liquidMasse moléculaire :154.1720X SSPE Buffer pH-7.4 suitable for molecular biology
The 20X SSC Buffer, with a pH between 6.9 and 7.1, is essential for in molecular biology, particularly for Southern and Northern hybridization techniques. This solution is sterilized through a 0.22 µm filter, making it suitable for direct use or for dilution based on experimental requirements. In parallel, 20X SSPE is available as a concentrated buffer intended for nucleic acid hybridizations and blot transfer processes. A significant feature of SSPE is its inclusion of EDTA, which chelates divalent metal ions like Mg²+. This property effectively inhibits DNase activity, helping to preserve the concentrations of probe and target DNA during Southern hybridization, thereby enhancing the accuracy of the results.Couleur et forme :Clear, Colourless, Liquid10X TE Buffer pH-8.0 for molecular biology
<p>TE buffer, often called T10E1 buffer is formulated from two primary components: Tris and EDTA. Tris serves to stabilize the pH, while EDTA protects DNA and RNA by chelating metal ions that are necessary for the activity of degrading enzymes, such as nucleases. This combination effectively preserves the integrity of nucleic acids. For optimal RNA protection, the solution should be maintained at a pH of 7.5, while DNA is best stored at a pH of 8.0. Importantly, a pH of 8.0 is also suitable for storing both DNA and RNA T10E1 buffer</p>Couleur et forme :Clear, Colourless, Liquid10X Tris-Tricine-SDS Buffer for molecular biology
<p>The Tris-Tricine-SDS Gel Running Buffer is specifically designed for separating proteins with molecular weights between 1 and 100 kDa, making it particularly effective for resolving proteins smaller than 30 kDa. This approach differs from traditional SDS-PAGE by substituting glycine (pK 9.6) with tricine (pK 8.15), which enhances the stacking and destacking of low molecular weight proteins and improves the resolution of smaller peptides due to the differing pK values. Incorporating urea into the stacking gel allows for the effective separation of two proteins that share the same molecular weight. Additionally, the lower acrylamide concentrations in Tricine gels facilitate the transfer of hydrophobic proteins during Western blotting. Tricine–SDS-PAGE is therefore commonly employed for the separation of lower molecular weight proteins.</p>Couleur et forme :Clear, Colourless, Liquid2.6M Tris Hydrochloride Buffer (2.6M Tris HCl) pH-8 for molecular biology
CAS :Couleur et forme :Clear, Colourless, LiquidAgarose Medium EEO for molecular biology
CAS :Couleur et forme :White to off-white, Free flowing powder, Clear, ColourlessNaphthol Blue Black B
CAS :Formule :C22H14N6Na2O9S2Degré de pureté :~ 80%Couleur et forme :Brown to black, PowderMasse moléculaire :616.5710X Tris Buffered Saline Tween-20 (TBST) for molecular biology
<p>10X Tris Buffered Saline Tween-20 (TBST) is a versatile wash solution widely used in Western blotting and ELISA. Its carefully balanced composition of pH stabilizers, salts, and detergents ensures efficient removal of unbound materials from membranes and microtiter plate wells. The Tris Buffered Saline with Tween 20 optimal formulation maintains a favorable buffering environment, preventing disruption of the critical antigen-antibody binding interactions. By effectively washing away excess components, TBST minimizes nonspecific background noise, thereby enhancing the signal-to-noise ratio and improving the overall sensitivity and accuracy of these assays.</p>Couleur et forme :Clear, Yellow tinted, LiquidAgarose High EEO for molecular biology
CAS :Couleur et forme :White, Free flowing powder, Clear, Colourless10X Tris Buffered Saline (TBS) for molecular biology
<p>Tris, short for tris(hydroxymethyl)aminomethane, is a commonly used primary amine known for its effectiveness as a buffering agent, particularly in the pH range of about 7.4, with a tolerance of ±0.1. This compound is advantageous because it does not precipitate calcium salts and aids in keeping manganese salts soluble. Additionally, Tris has a low UV absorbance, making it suitable for various experimental applications, although it is notably sensitive to changes in temperature. These characteristics contribute to its widespread use in biochemical and molecular biology experiments.</p>Couleur et forme :Clear, Colourless, LiquidN,N-Methylene Bisacrylamide (bis-Acrylamide) 3x cryst. extrapure AR, 99.5%
CAS :Formule :C7H10N2O2Degré de pureté :min. 99.5%Couleur et forme :White, Crystalline powder, Clear, ColourlessMasse moléculaire :154.1710X Tris-Glycine-SDS Buffer for molecular biology
<p>SDS-PAGE is a method for separating proteins according to their movement in an electrical current and their molecular mass, which relates to the length of their polypeptide chains. A widely recognized system for this technique is the Laemmli system, introduced in 1970. It employs Tris-Glycine gels made up of a stacking gel and a resolving gel, utilizing varying acrylamide concentrations to achieve separation based on molecular weight. This traditional system incorporates a discontinuous buffer setup, with different pH levels and ionic strengths for the buffers: the running buffer (10X Tris-Glycine-SDS Gel Running Buffer) is set at pH 8.3, while the stacking gel operates at pH 6.8, and the resolving gel is at pH 8.8.</p>Couleur et forme :Clear, Colourless, Liquid3,3'-Diethyloxadicarbocyanine Iodide
CAS :Formule :C23H23IN2O2Degré de pureté :>98.0%(N)Couleur et forme :Blue to Dark blue powder to crystalMasse moléculaire :486.35Cetyltrimethyl Ammonium Chloride (CTAC) for molecular biology, 99%
CAS :Formule :C19H42NClDegré de pureté :min. 99%Couleur et forme :White, Crystalline powder, Clear, ColourlessMasse moléculaire :320.00Ethidium Bromide extrapure, 95%
CAS :Formule :C21H20N3BrDegré de pureté :min.95%Couleur et forme :Red, Crystalline Powder, Clear, RedMasse moléculaire :394.32N,N,N-Trimethylethylenediamine pure, 97%
CAS :Formule :C5H14N2Degré de pureté :min. 97%Couleur et forme :Clear, Colourless, LiquidMasse moléculaire :102.18N,N,N,N-Tetramethyl Ethylenediamine (TEMED) extrapure AR, ExiPlus, Multi-Compendial, 99%
CAS :Formule :C6H16N2Degré de pureté :min. 99%Couleur et forme :Clear, Colourless, LiquidMasse moléculaire :116.21Ammonium Persulphate (APS) for electrophoresis, 99%
CAS :Formule :N2H8S2O8Degré de pureté :min. 99%Couleur et forme :White, Crystalline powder, Clear, ColourlessMasse moléculaire :228.20N,N,N,N-Tetramethyl Ethylenediamine (TEMED) for molecular biology, 99.5%
CAS :Formule :C6H16N2Degré de pureté :min. 99.5%Couleur et forme :Clear, Colourless, LiquidMasse moléculaire :116.21Ethidium Bromide Solution (10mg/ml)
Formule :C21H20N3BrCouleur et forme :Dark red, Clear, LiquidMasse moléculaire :394.32Sodium Lauryl Sulphate (SDS, SLS) extrapure AR, ACS, 99%
CAS :Formule :C12H25SO4NaDegré de pureté :min.99%Couleur et forme :White, Crystalline powder, Clear, ColourlessMasse moléculaire :288.38Acrylamide 3x cryst. extrapure AR, 99.9%
CAS :Formule :C3H5NODegré de pureté :min. 99.9%Couleur et forme :White, Crystalline powder, Clear, ColourlessMasse moléculaire :71.0830% Acrylamide / Bis-acrylamide Mix Solution (Ratio 29:1)
SDS-PAGE is a method employed for separating proteins via electrophoresis, based on the principle that charged molecules migrate through a matrix in response to an applied electric field. The matrix utilized for this separation is polyacrylamide, which is derived from acrylamide, a compound that can be hazardous. Polyacrylamide is commonly used for the size-based separation of proteins and nucleic acids. The gel matrix forms through the free radical polymerization of acrylamide along with a crosslinking agent known as N, N-Methylenebisacrylamide. In this process, acrylamide monomers polymerize into long chains, initiated by a free radical-generating system, and these chains are linked by the cross-linker, resulting in a gel structure. This gel is crucial for effective electrophoretic separation, facilitating the analysis of biomolecules based on size.Couleur et forme :Clear, Colourless, LiquidCetyltrimethyl Ammonium Bromide (CTAB) for molecular biology, 99%
CAS :Formule :C19H42BrNDegré de pureté :min. 99.5%Couleur et forme :White, Crystalline powder, Clear, Colourless, Clear, ColourlessMasse moléculaire :364.45
![Glycerol [for Electrophoresis]](/_next/image/?url=https%3A%2Fstatic.cymitquimica.com%2Fproducts%2F3B%2Fthumb-webp%2FG0316.webp&w=3840&q=75)
![3,3'-Diaminobenzidine [for Biochemical Research]](/_next/image/?url=https%3A%2Fstatic.cymitquimica.com%2Fproducts%2F3B%2Fthumb-webp%2FD3756.webp&w=3840&q=75)
![Acrylamide Monomer [for Electrophoresis]](/_next/image/?url=https%3A%2Fstatic.cymitquimica.com%2Fproducts%2F3B%2Fthumb-webp%2FA1132.webp&w=3840&q=75)
![Fast Green FCF [for Biochemical Research]](/_next/image/?url=https%3A%2Fstatic.cymitquimica.com%2Fproducts%2F3B%2Fthumb-webp%2FF0718.webp&w=3840&q=75)
![TCEP Hydrochloride [for Electrophoresis]](/_next/image/?url=https%3A%2Fstatic.cymitquimica.com%2Fproducts%2F3B%2Fthumb-webp%2FT4134.webp&w=3840&q=75)
![Western Blot Stripping Buffer [for Biochemical Research]](/_next/image/?url=https%3A%2Fstatic.cymitquimica.com%2Fproducts%2F3B%2Fthumb-webp%2FW0024.webp&w=3840&q=75)
![1-Thioglycerol [for Electrophoresis]](/_next/image/?url=https%3A%2Fstatic.cymitquimica.com%2Fproducts%2F3B%2Fthumb-webp%2FT3843.webp&w=3840&q=75)
