Électrophorèse
Dans cette section, vous trouverez tous les réactifs et matériaux nécessaires pour réaliser l'électrophorèse, une technique de laboratoire essentielle utilisée pour séparer les protéines en fonction de leur masse moléculaire et de leur charge. Cette technique est cruciale pour analyser la composition et la pureté des échantillons de protéines, ainsi que pour étudier les acides nucléiques et autres biomolécules. Notre sélection comprend des tampons d'électrophorèse, des gels, des colorants et des marqueurs de haute qualité, ainsi que des appareils et accessoires conçus pour garantir une séparation précise et efficace. Ces produits sont indispensables pour la recherche en biologie moléculaire, biochimie et génétique, fournissant des résultats fiables pour la caractérisation des protéines, l'analyse de l'expression génique et d'autres applications. Chez CymitQuimica, nous fournissons tout ce dont vous avez besoin pour réaliser l'électrophorèse, soutenant vos recherches avec précision et cohérence.
Sous-catégories appartenant à la catégorie "Électrophorèse"
299 produits trouvés pour "Électrophorèse"
Trier par
Degré de pureté (%)
0
100
|
0
|
50
|
90
|
95
|
100
1M Tris Hydrochloride Buffer (1M Tris HCl) pH-7.2 for molecular biology
Couleur et forme :Clear, Colourless, LiquidBrilliant Blue R-250 for molecular biology
CAS :Formule :C45H44N3NaO7S2Couleur et forme :Dark blue with reddish tinge, PowderMasse moléculaire :825.9810X Tris-Tricine-SDS Buffer for molecular biology
The Tris-Tricine-SDS Gel Running Buffer is specifically designed for separating proteins with molecular weights between 1 and 100 kDa, making it particularly effective for resolving proteins smaller than 30 kDa. This approach differs from traditional SDS-PAGE by substituting glycine (pK 9.6) with tricine (pK 8.15), which enhances the stacking and destacking of low molecular weight proteins and improves the resolution of smaller peptides due to the differing pK values. Incorporating urea into the stacking gel allows for the effective separation of two proteins that share the same molecular weight. Additionally, the lower acrylamide concentrations in Tricine gels facilitate the transfer of hydrophobic proteins during Western blotting. Tricine–SDS-PAGE is therefore commonly employed for the separation of lower molecular weight proteins.Couleur et forme :Clear, Colourless, Liquid10X MOPS Buffer for molecular biology
MOPS, or morpholino propanesulfonic acid, is a structural analog of MES, the ethanesulfonic acid. With a pKa value of 6.80-7.20, MOPS serves as an excellent buffer for many biological systems at near-neutral pH. It is extensively used as a buffering agent in molecular biology and biochemistry and has been tested and recommended for use in polyacrylamide gel electrophoresis. Additionally, MOPS is applicable in various bioanalytical methods, including isoelectric focusing, protein assays, and X-ray crystallographic studies. Furthermore, it can be used as an electrophoresis buffer for agarose gel electrophoresis of RNA.Couleur et forme :Clear, Colourless, Liquid20X SSC Buffer pH 6.9-7.1 for molecular biology
The Saline-Sodium Citrate Hybridization Buffer [20X] is a key component in various nucleic acid techniques, ensuring optimal stringency during washing steps in procedures such as Southern blotting, in situ hybridization, DNA microarrays, and Northern blotting. When utilized as 20X SSC, it effectively prevents agarose gels from drying out during vacuum transfers, preserving sample integrity. This solution is specifically formulated to support the diverse needs of end users in nucleic acid hybridizations and blot transfer applications, making it an invaluable resource in molecular biology workflows.Couleur et forme :Clear, Colourless, Solution10X Tris Buffered Saline Tween-20 (TBST) for molecular biology
10X Tris Buffered Saline Tween-20 (TBST) is a versatile wash solution widely used in Western blotting and ELISA. Its carefully balanced composition of pH stabilizers, salts, and detergents ensures efficient removal of unbound materials from membranes and microtiter plate wells. The Tris Buffered Saline with Tween 20 optimal formulation maintains a favorable buffering environment, preventing disruption of the critical antigen-antibody binding interactions. By effectively washing away excess components, TBST minimizes nonspecific background noise, thereby enhancing the signal-to-noise ratio and improving the overall sensitivity and accuracy of these assays.Couleur et forme :Clear, Yellow tinted, LiquidAlbumin Bovine Solution 10% Diluent Solution in PBS (BSA 10% Diluent/Blocking Solution in PBS)
CAS :Couleur et forme :Clear, Pale yellow, LiquidAgarose High EEO for molecular biology
CAS :Couleur et forme :White, Free flowing powder, Clear, Colourless2.6M Tris Hydrochloride Buffer (2.6M Tris HCl) pH-8 for molecular biology
CAS :Couleur et forme :Clear, Colourless, Liquid10X Tris Buffered Saline (TBS) for molecular biology
Tris, short for tris(hydroxymethyl)aminomethane, is a commonly used primary amine known for its effectiveness as a buffering agent, particularly in the pH range of about 7.4, with a tolerance of ±0.1. This compound is advantageous because it does not precipitate calcium salts and aids in keeping manganese salts soluble. Additionally, Tris has a low UV absorbance, making it suitable for various experimental applications, although it is notably sensitive to changes in temperature. These characteristics contribute to its widespread use in biochemical and molecular biology experiments.Couleur et forme :Clear, Colourless, Liquid10X TE Buffer pH-8.0 for molecular biology
TE buffer, often called T10E1 buffer is formulated from two primary components: Tris and EDTA. Tris serves to stabilize the pH, while EDTA protects DNA and RNA by chelating metal ions that are necessary for the activity of degrading enzymes, such as nucleases. This combination effectively preserves the integrity of nucleic acids. For optimal RNA protection, the solution should be maintained at a pH of 7.5, while DNA is best stored at a pH of 8.0. Importantly, a pH of 8.0 is also suitable for storing both DNA and RNA T10E1 bufferCouleur et forme :Clear, Colourless, Liquid
