Électrophorèse
Dans cette section, vous trouverez tous les réactifs et matériaux nécessaires pour réaliser l'électrophorèse, une technique de laboratoire essentielle utilisée pour séparer les protéines en fonction de leur masse moléculaire et de leur charge. Cette technique est cruciale pour analyser la composition et la pureté des échantillons de protéines, ainsi que pour étudier les acides nucléiques et autres biomolécules. Notre sélection comprend des tampons d'électrophorèse, des gels, des colorants et des marqueurs de haute qualité, ainsi que des appareils et accessoires conçus pour garantir une séparation précise et efficace. Ces produits sont indispensables pour la recherche en biologie moléculaire, biochimie et génétique, fournissant des résultats fiables pour la caractérisation des protéines, l'analyse de l'expression génique et d'autres applications. Chez CymitQuimica, nous fournissons tout ce dont vous avez besoin pour réaliser l'électrophorèse, soutenant vos recherches avec précision et cohérence.
Sous-catégories appartenant à la catégorie "Électrophorèse"
298 produits trouvés pour "Électrophorèse"
Trier par
Degré de pureté (%)
0
100
|
0
|
50
|
90
|
95
|
100
10X Tris-Glycine pH-8.3 Tank Buffer for molecular biology
<p>The 10X Tris-Glycine Buffer, with a pH of 8.3, is designed specifically for use in native PAGE gel electrophoresis. This buffer offers great convenience, as it can be diluted with distilled water or another suitable solvent according to the specific application prior to electrophoresis. Additionally, it is useful for the transfer of protein samples onto nitrocellulose membranes during western blotting. To prevent the gel from swelling during the transfer process and to enhance the binding of protein samples to nitrocellulose, the inclusion of methanol is essential.</p>Couleur et forme :Clear, Colourless, Liquid10X TE Buffer pH-8.0 for molecular biology
<p>TE buffer, often called T10E1 buffer is formulated from two primary components: Tris and EDTA. Tris serves to stabilize the pH, while EDTA protects DNA and RNA by chelating metal ions that are necessary for the activity of degrading enzymes, such as nucleases. This combination effectively preserves the integrity of nucleic acids. For optimal RNA protection, the solution should be maintained at a pH of 7.5, while DNA is best stored at a pH of 8.0. Importantly, a pH of 8.0 is also suitable for storing both DNA and RNA T10E1 buffer</p>Couleur et forme :Clear, Colourless, Liquid10X Tris Buffered Saline (TBS) for molecular biology
<p>Tris, short for tris(hydroxymethyl)aminomethane, is a commonly used primary amine known for its effectiveness as a buffering agent, particularly in the pH range of about 7.4, with a tolerance of ±0.1. This compound is advantageous because it does not precipitate calcium salts and aids in keeping manganese salts soluble. Additionally, Tris has a low UV absorbance, making it suitable for various experimental applications, although it is notably sensitive to changes in temperature. These characteristics contribute to its widespread use in biochemical and molecular biology experiments.</p>Couleur et forme :Clear, Colourless, LiquidAgarose High EEO for molecular biology
CAS :Couleur et forme :White, Free flowing powder, Clear, Colourless10X Tris Buffered Saline Tween-20 (TBST) for molecular biology
<p>10X Tris Buffered Saline Tween-20 (TBST) is a versatile wash solution widely used in Western blotting and ELISA. Its carefully balanced composition of pH stabilizers, salts, and detergents ensures efficient removal of unbound materials from membranes and microtiter plate wells. The Tris Buffered Saline with Tween 20 optimal formulation maintains a favorable buffering environment, preventing disruption of the critical antigen-antibody binding interactions. By effectively washing away excess components, TBST minimizes nonspecific background noise, thereby enhancing the signal-to-noise ratio and improving the overall sensitivity and accuracy of these assays.</p>Couleur et forme :Clear, Yellow tinted, LiquidNaphthol Blue Black B
CAS :Formule :C22H14N6Na2O9S2Degré de pureté :~ 80%Couleur et forme :Brown to black, PowderMasse moléculaire :616.57Agarose Medium EEO for molecular biology
CAS :Couleur et forme :White to off-white, Free flowing powder, Clear, ColourlessN,N'-Methylene Bisacrylamide 2% Aq. Solution for molecular biology
CAS :Formule :C7H10N2O2Couleur et forme :Clear, Colourless, liquidMasse moléculaire :154.171X Phosphate Buffered Saline (PBS) for molecular biology
<p>The pH of the buffer is typically maintained within a range of 7.4 ± 0.1, closely resembling the physiological pH found in many biological systems.</p>Couleur et forme :Clear, Colourless, Liquid10X Phosphate Buffered Saline Tween-20 (PBST) for molecular biology
Couleur et forme :Clear, Colourless with yellow tinted, Liquid1M Tris Hydrochloride Buffer (1M Tris HCl) pH-7.2 for molecular biology
Couleur et forme :Clear, Colourless, LiquidBrilliant Blue R-250 for molecular biology
CAS :Formule :C45H44N3NaO7S2Couleur et forme :Dark blue with reddish tinge, PowderMasse moléculaire :825.9810X MOPS Buffer for molecular biology
<p>MOPS, or morpholino propanesulfonic acid, is a structural analog of MES, the ethanesulfonic acid. With a pKa value of 6.80-7.20, MOPS serves as an excellent buffer for many biological systems at near-neutral pH. It is extensively used as a buffering agent in molecular biology and biochemistry and has been tested and recommended for use in polyacrylamide gel electrophoresis. Additionally, MOPS is applicable in various bioanalytical methods, including isoelectric focusing, protein assays, and X-ray crystallographic studies. Furthermore, it can be used as an electrophoresis buffer for agarose gel electrophoresis of RNA.</p>Couleur et forme :Clear, Colourless, Liquid20X SSC Buffer pH 6.9-7.1 for molecular biology
<p>The Saline-Sodium Citrate Hybridization Buffer [20X] is a key component in various nucleic acid techniques, ensuring optimal stringency during washing steps in procedures such as Southern blotting, in situ hybridization, DNA microarrays, and Northern blotting. When utilized as 20X SSC, it effectively prevents agarose gels from drying out during vacuum transfers, preserving sample integrity. This solution is specifically formulated to support the diverse needs of end users in nucleic acid hybridizations and blot transfer applications, making it an invaluable resource in molecular biology workflows.</p>Couleur et forme :Clear, Colourless, SolutionLithium Lauryl Sulphate extrapure, 99%
CAS :Formule :C12H25SO4LiDegré de pureté :min. 99%Couleur et forme :White, Crystalline powder, Clear, ColourlessMasse moléculaire :272.30Albumin Bovine Solution 10% Diluent Solution in PBS (BSA 10% Diluent/Blocking Solution in PBS)
CAS :Couleur et forme :Clear, Pale yellow, Liquid1M Tris Hydrochloride Buffer (1M Tris HCl) pH-8.0 for molecular biology
Couleur et forme :Clear, Colourless, Liquid20X SSPE Buffer pH-7.4 suitable for molecular biology
The 20X SSC Buffer, with a pH between 6.9 and 7.1, is essential for in molecular biology, particularly for Southern and Northern hybridization techniques. This solution is sterilized through a 0.22 µm filter, making it suitable for direct use or for dilution based on experimental requirements. In parallel, 20X SSPE is available as a concentrated buffer intended for nucleic acid hybridizations and blot transfer processes. A significant feature of SSPE is its inclusion of EDTA, which chelates divalent metal ions like Mg²+. This property effectively inhibits DNase activity, helping to preserve the concentrations of probe and target DNA during Southern hybridization, thereby enhancing the accuracy of the results.Couleur et forme :Clear, Colourless, Liquid10X Tris-Tricine-SDS Buffer for molecular biology
<p>The Tris-Tricine-SDS Gel Running Buffer is specifically designed for separating proteins with molecular weights between 1 and 100 kDa, making it particularly effective for resolving proteins smaller than 30 kDa. This approach differs from traditional SDS-PAGE by substituting glycine (pK 9.6) with tricine (pK 8.15), which enhances the stacking and destacking of low molecular weight proteins and improves the resolution of smaller peptides due to the differing pK values. Incorporating urea into the stacking gel allows for the effective separation of two proteins that share the same molecular weight. Additionally, the lower acrylamide concentrations in Tricine gels facilitate the transfer of hydrophobic proteins during Western blotting. Tricine–SDS-PAGE is therefore commonly employed for the separation of lower molecular weight proteins.</p>Couleur et forme :Clear, Colourless, Liquid2.6M Tris Hydrochloride Buffer (2.6M Tris HCl) pH-8 for molecular biology
CAS :Couleur et forme :Clear, Colourless, LiquidN,N-Methylene Bisacrylamide (bis-Acrylamide) 3x cryst. extrapure AR, 99.5%
CAS :Formule :C7H10N2O2Degré de pureté :min. 99.5%Couleur et forme :White, Crystalline powder, Clear, ColourlessMasse moléculaire :154.1710X Tris-Glycine-SDS Buffer for molecular biology
<p>SDS-PAGE is a method for separating proteins according to their movement in an electrical current and their molecular mass, which relates to the length of their polypeptide chains. A widely recognized system for this technique is the Laemmli system, introduced in 1970. It employs Tris-Glycine gels made up of a stacking gel and a resolving gel, utilizing varying acrylamide concentrations to achieve separation based on molecular weight. This traditional system incorporates a discontinuous buffer setup, with different pH levels and ionic strengths for the buffers: the running buffer (10X Tris-Glycine-SDS Gel Running Buffer) is set at pH 8.3, while the stacking gel operates at pH 6.8, and the resolving gel is at pH 8.8.</p>Couleur et forme :Clear, Colourless, Liquid3,3'-Diethyloxadicarbocyanine Iodide
CAS :Formule :C23H23IN2O2Degré de pureté :>98.0%(N)Couleur et forme :Blue to Dark blue powder to crystalMasse moléculaire :486.35N,N,N,N-Tetramethyl Ethylenediamine (TEMED) extrapure AR, ExiPlus, Multi-Compendial, 99%
CAS :Formule :C6H16N2Degré de pureté :min. 99%Couleur et forme :Clear, Colourless, LiquidMasse moléculaire :116.2130% Acrylamide / Bis-acrylamide Mix Solution (Ratio 29:1)
<p>SDS-PAGE is a method employed for separating proteins via electrophoresis, based on the principle that charged molecules migrate through a matrix in response to an applied electric field. The matrix utilized for this separation is polyacrylamide, which is derived from acrylamide, a compound that can be hazardous. Polyacrylamide is commonly used for the size-based separation of proteins and nucleic acids. The gel matrix forms through the free radical polymerization of acrylamide along with a crosslinking agent known as N, N-Methylenebisacrylamide. In this process, acrylamide monomers polymerize into long chains, initiated by a free radical-generating system, and these chains are linked by the cross-linker, resulting in a gel structure. This gel is crucial for effective electrophoretic separation, facilitating the analysis of biomolecules based on size.</p>Couleur et forme :Clear, Colourless, LiquidCetyltrimethyl Ammonium Bromide (CTAB) for molecular biology, 99%
CAS :Formule :C19H42BrNDegré de pureté :min. 99.5%Couleur et forme :White, Crystalline powder, Clear, Colourless, Clear, ColourlessMasse moléculaire :364.45Ethidium Bromide extrapure, 95%
CAS :Formule :C21H20N3BrDegré de pureté :min.95%Couleur et forme :Red, Crystalline Powder, Clear, RedMasse moléculaire :394.32Cetyltrimethyl Ammonium Chloride (CTAC) for molecular biology, 99%
CAS :Formule :C19H42NClDegré de pureté :min. 99%Couleur et forme :White, Crystalline powder, Clear, ColourlessMasse moléculaire :320.00Acrylamide 3x cryst. extrapure AR, 99.9%
CAS :Formule :C3H5NODegré de pureté :min. 99.9%Couleur et forme :White, Crystalline powder, Clear, ColourlessMasse moléculaire :71.08N,N,N,N-Tetramethyl Ethylenediamine (TEMED) for molecular biology, 99.5%
CAS :Formule :C6H16N2Degré de pureté :min. 99.5%Couleur et forme :Clear, Colourless, LiquidMasse moléculaire :116.21Ethidium Bromide Solution (10mg/ml)
Formule :C21H20N3BrCouleur et forme :Dark red, Clear, LiquidMasse moléculaire :394.32N,N,N-Trimethylethylenediamine pure, 97%
CAS :Formule :C5H14N2Degré de pureté :min. 97%Couleur et forme :Clear, Colourless, LiquidMasse moléculaire :102.18Sodium Lauryl Sulphate (SDS, SLS) extrapure AR, ACS, 99%
CAS :Formule :C12H25SO4NaDegré de pureté :min.99%Couleur et forme :White, Crystalline powder, Clear, ColourlessMasse moléculaire :288.38Ammonium Persulphate (APS) for electrophoresis, 99%
CAS :Formule :N2H8S2O8Degré de pureté :min. 99%Couleur et forme :White, Crystalline powder, Clear, ColourlessMasse moléculaire :228.20
