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Aldol® 467 N-acetyl-beta-D-glucosaminide is a chromogenic and fluorogenic enzyme substrate used for detection of beta-N-acetyl-glucosaminidase activity. The colorless enzyme substrate is cleaved in the presence of beta-N-acetyl-glucosaminidase yielding development of a yellow product. In addition, green fluorescence is generated when a suitable matrix or Aldol® 355 fluorescence enhancer is present. Aldol® 467 N-acetyl-beta-D-glucosaminide amide can be used in enzymatic assays or in live bacterial cultures. Aldol® 467 N-acetyl-beta-D-glucosaminide is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.

4-Nitrophenyl 4,6-O-isopropylidene-a-D-galactopyranoside is a fluorogenic substrate that can be used to measure the activity of beta-galactosidase. This product is high purity, high quality and has a CAS number of 29781-31-5. It is an enzyme substrate with chromogenic and diagnostic properties that are used for conjugation to other molecules, as well as for culture media, chemiluminescence and food testing. 4NP4IPG is also used in bioluminescence assays to detect the presence of beta-galactosidase.

Aldol® 515 N-glutaryl-L-phenylalanine amide is a chromogenic and fluorogenic enzyme substrate used for detection of L-glutamate-phenylalanine (GluPhe)-specific peptidase activity. The colorless enzyme substrate is cleaved the GluPhe-specific peptidase, yielding development of orange-red coloration. In addition, red fluorescence is generated when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 515 N-glutaryl-L-phenylalanine amide can be used in enzyme assays and live bacterial cultures. Aldol® 515 N-glutaryl-L-phenylalanine amide is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.

2-Chloro-4-nitrophenyl β-D-xylopyranoside is a fluorogenic substrate that can be used for the detection of β-glucuronidase activity in food testing, environmental testing, and diagnostics. It has been shown to be an effective substrate for detecting β-glucuronidase activity with a high degree of sensitivity and specificity. 2CNPX can also be used as a chromogenic substrate for the detection of β-glucuronidase activity in food testing and environmental testing. The conjugate form of this substrate is useful for immunochemical assays, such as enzyme linked immunosorbent assay (ELISA) or radioimmunoassay (RIA). This product is also used as a ligand in fluorescence resonance energy transfer (FRET) assays.
2CNPX can be used to detect β-glucuronidase activity by chemiluminescence

4-Nitrophenyl b-D-thiomannopyranoside acts as a substrate for β-mannosidase and thioglycoligase. Upon enzymatic cleavage, these substrates release 4-nitrophenol, a yellow chromophore that can be detected spectrophotometrically at 405 nm, facilitating quantitative measurements of enzymatic activity. Consequently, these substrates are widely used in biochemical research, clinical diagnostics, and drug discovery for evaluating the function of specific enzymes in various biological processes.

4-Nitrophenyl 2-O-(2,3,4,6-tetra-O-acetyl-a-D-mannopyranosyl)-a-D-mannopyranoside is a fluorogenic substrate that is used as a diagnostic tool and can be used to detect the presence of β--glucosidase in the gut. It is also used as a conjugate with enzymes such as peroxidase or alkaline phosphatase for use in enzyme catalyzed reactions. The product has been shown to be active against a variety of bacteria, including Staphylococcus aureus and Escherichia coli. This product has been shown to produce chemiluminescence when incubated with horseradish peroxidase and hydrogen peroxide. This product is light sensitive and should be stored at room temperature away from direct sunlight.

4-Nitrophenyl 6-O-DMT-2,3,4-tri-O-levulinoyl-a-D-mannopyranoside is a fluorogenic substrate that can be used for the detection of DMT (dihydroxyacetone) in food products. It has been shown to be an excellent alternative to the more commonly used 4,5′,6,7,8,-Tetrabromoindoxyl 3′,5′,6′,-tetraacetic acid (TBA). This product is also used as a chromogenic substrate for the determination of phenols in environmental testing and as a ligand conjugate for immunohistochemistry staining. The purity of this product is guaranteed and it has been tested extensively.

4-Nitrophenyl 2-acetamido-2-deoxy-3-O-(a-D-galactopyranosyl)-a-D-galactopyranoside is a fluorescent substrate that can be used in chemiluminescent assays. It is a high purity, high quality chemical that has been tested for culture media, environmental testing and bioluminescence. This product is an excellent choice for use in diagnostics, chromogenic substrates, enzyme substrates and food testing.

Aldol® 470 L-pyroglutamic acid amide is a chromogenic and fluorogenic enzyme substrate used for detection of pyrrolidonyl peptidase (PYRase) activity. The colorless enzyme substrate is cleaved by PYRase-producing bacteria grown in liquid or solid media, yielding orange color. In addition, green fluorescence is generated when a suitable matrix is present, such as, the surface of cellulose acetate filters, polypropylene tubes or microplates, and polyethylene vials. Aldol® 470 L-pyroglutamic acid amide is suitable for use under aerobic and anaerobic conditions.

Chromogenic substrate targeting amylase and maltase

4-Nitrophenyl beta-galactoside (pNP-Gal) is a chromogenic substrate commonly used in enzymatic assays to detect and quantify the presence of beta-galactosidase. It is hydrolyzed by the enzyme to release 4-nitrophenol, which produces a yellow color that can be measured spectrophotometrically. Similarly, 4-Nitrophenyl beta-glucoside (pNP-Glc) can be used to assay beta-glucosidase activity. 4-Nitrophenyl beta-globotrioside (pNP-Glo) is a similar compound that has been designed as a substrate for glycosidases that cleave terminal beta-galactosyl-(1->3)-N-acetylgalactosamine residues. In these assays, the release of 4-nitrophenol can be measured to determine the activity of the enzyme under study. The use of chromogenic substrates like pNP-Glo allows for the rapid and reliable detection of glycosidase activity in a variety of biological samples.

4-Nitrophenyl alpha-D-maltotetraoside is a chromogenic substrate used for the detection of alpha-glucosidase and alpha-amylase activity. The four glucose units in the molecule are hydrolyzed by the enzyme, releasing 4-nitrophenol and allowing for easy colorimetric measurement of enzymatic activity

Modified naphtol AS substrate used in histochemical and cytochemical staining

1-Naphthyl-alpha-D-mannopyranoside is a chromogenic substrate. It is used in diagnostics and as a reagent for enzymatic analysis of alpha-mannosidase activity in culture media, food testing, and environmental testing.

4-Nitrophenyl-myo-inositol-1-yl phosphate is a highly specific chromogenic substrate used for the detection, quantification, and characterization of phosphoinositide phosphatases. When cleaved by the target enzyme, it generates a highly absorbing 4-nitrophenyl moiety, producing a yellow color that can be measured spectrophotometrically. This versatile substrate is valuable in examining phosphoinositide phosphatase activity, enzyme kinetics, substrate specificity, and inhibition studies in various cellular processes, including signal transduction, lipid metabolism, and membrane trafficking.