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2-Nitrophenyl-2,3,4-tri-O-acetyl-b-D-glucuronide methyl ester is a tri-O-acetylated glucuronide substrate commonly used in chromogenic assays to detect the activity of beta-glucuronidase. Upon hydrolysis by the enzyme, the substrate releases 2-nitrophenol, which can be spectrophotometrically. The substrate is widely used in diagnostic tests to assess bacterial and fungal infections, as well as in drug metabolism studies.

5-Bromo-3-indoxyl-beta-D-galactopyranoside (5BIG) is a fluorogenic substrate that has been shown to be an effective enzyme substrate. It is used in the chemiluminescence, bioluminescence, and fluorescence detection of enzymes. 5BIG is also a chromogenic substrate for nucleic acid staining and can be used in food testing as well as diagnostics. 5BIG is highly pure and high quality with a CAS number of 1301706-59-1.

Chromogenic substrate targeting amylase and maltase

4-Nitrophenyl b-D-thiomannopyranoside acts as a substrate for β-mannosidase and thioglycoligase. Upon enzymatic cleavage, these substrates release 4-nitrophenol, a yellow chromophore that can be detected spectrophotometrically at 405 nm, facilitating quantitative measurements of enzymatic activity. Consequently, these substrates are widely used in biochemical research, clinical diagnostics, and drug discovery for evaluating the function of specific enzymes in various biological processes.

4-Nitrophenyl 2-O-(2,3,4,6-tetra-O-acetyl-a-D-mannopyranosyl)-a-D-mannopyranoside is a fluorogenic substrate that is used as a diagnostic tool and can be used to detect the presence of β--glucosidase in the gut. It is also used as a conjugate with enzymes such as peroxidase or alkaline phosphatase for use in enzyme catalyzed reactions. The product has been shown to be active against a variety of bacteria, including Staphylococcus aureus and Escherichia coli. This product has been shown to produce chemiluminescence when incubated with horseradish peroxidase and hydrogen peroxide. This product is light sensitive and should be stored at room temperature away from direct sunlight.

4-Nitrophenyl 4,6-O-benzylidene-b-D-glucopyranoside is a fluorescent compound that is used in diagnostics and research. The compound is an enzyme substrate, a ligand for metal ions, and a chromogenic substrate. It can be used to test cultures of bacteria in culture media, as well as for food testing and environmental testing. 4-Nitrophenyl 4,6-O-benzylidene-b-D-glucopyranoside has high purity and quality due to its high fluorescence intensity. This product has been shown to be highly stable in various conditions and may be used in conjugates with other compounds.

Aldol® 455 is a substrate for the enzyme beta-galactosidase. It is used in food testing, staining, and environmental testing. Aldol® 455 has been shown to be a good substrate for fluorogenic and chromogenic substrates. It can be conjugated with an antibody or other ligand for use in immunoassays. Aldol® 455 is of high purity and high quality, making it suitable for use in diagnostics and as an enzyme substrate. This product can also be used as a culture media additive.

4-Nitrophenyl trimethylacetate is a cytosolic proteinase inhibitor. It binds to the active site of trypsin and other enzymes that hydrolyze peptide bonds, thereby inhibiting the activity of these enzymes. 4-Nitrophenyl trimethylacetate has been shown to inhibit soybean trypsin, which has led to its use as a model system for studying enzyme inhibition. The binding site on 4-Nitrophenyl trimethylacetate is competitive with respect to substrate binding but not with respect to the enzyme's catalytic triad. This inhibitor also has a high buffer capacity and can be used in low pH systems.

2-Nitrophenyl 2-acetamido-2-deoxy-b-D-galactopyranoside is a carboxymethyl derivative of the natural substrate of β-galactosidase, which is used as a substrate for the production of recombinant proteins in the corynebacterium glutamicum. The enzyme β-galactosidase hydrolyzes this substrate to produce an acid molecule and a sugar molecule. This product can be used in biotechnological processes for the production of recombinant proteins or other bioactive molecules. It can also be used in the isolation of nucleic acid from bacteria or fungi, or to study bacterial membrane transport systems. 2NPGAG can be expressed as a recombinant protein with expression vectors such as pET21a and pET24a. It has been shown that this product inhibits transport through membranes by binding to cellulose, which may be useful for studies on cellulose biosynthesis

3-Indolyl 2-acetamido-2-deoxy-b-D-galactopyranoside is a fluorogenic substrate that reacts with the enzyme β-galactosidase to produce a blue product. This product can be used to diagnose the presence of β-galactosidase in culture media, as well as in food and environmental testing. 3IAG is also a chromogenic substrate that can be used for detection of β-galactosidase activity.

4-Nitrophenyl 2-acetamido-3-O-(3,4,6-tri-O-acetyl-2-deoxy-2-phthalimido-b-D-glucopyranosyl)-2-deoxy--4,6--O--4--methoxybenzylidene--a--D--galactopyranoside is a chemiluminescent substrate for the enzyme beta galactosidase. It reacts with this enzyme to form 4 nitrophenol and acetamide. 4 Nitrophenyl 2 acetamido 3 O (3,4,6 tri O acetyl 2 deoxy 2 phthalimido b D glucopyranosyl) 2 deoxy 4 6 O 4 methoxybenzylidene a D galactopyranoside is used as a chromogenic substrate in diagnostics and as an environmental testing agent. It can be used as an imaging

4-Nitrophenyl b-D-cellohexaoside is a substrate for many enzymes and can be used to detect the presence of these enzymes in biological samples. It can also be used to detect the presence of bacteria, fungi, parasites, and viruses. 4-Nitrophenyl b-D-cellohexaoside is an excellent substrate for enzyme reactions that produce a chromogenic or fluorogenic product. This product is often conjugated with other compounds such as antibodies or antigens to form an immunoassay. 4-Nitrophenyl b-D-cellohexaoside is made from a high purity chemical by a process called nitration with sodium nitrate and sulfuric acid.

4-Nitrophenyl 4,6-O-isopropylidene-a-D-galactopyranoside is a fluorogenic substrate that can be used to measure the activity of beta-galactosidase. This product is high purity, high quality and has a CAS number of 29781-31-5. It is an enzyme substrate with chromogenic and diagnostic properties that are used for conjugation to other molecules, as well as for culture media, chemiluminescence and food testing. 4NP4IPG is also used in bioluminescence assays to detect the presence of beta-galactosidase.

5-Bromo-4-chloro-3-indoxyl-a-D-xylopyranoside is a fluorogenic substrate that can be used in food testing and environmental testing. This product is a conjugate of the xylosidase enzyme (EC 3.2.1.37) with 5-bromo-4-chloroindoxyl, which has been shown to have a high affinity for the enzyme's active site and enzymatic activity. The purity of this product is > 95% and the CAS number is 68618-41-5.

4-Nitrophenyl alpha-D-maltotetraoside is a chromogenic substrate used for the detection of alpha-glucosidase and alpha-amylase activity. The four glucose units in the molecule are hydrolyzed by the enzyme, releasing 4-nitrophenol and allowing for easy colorimetric measurement of enzymatic activity

1-Naphthyl-alpha-D-mannopyranoside is a chromogenic substrate. It is used in diagnostics and as a reagent for enzymatic analysis of alpha-mannosidase activity in culture media, food testing, and environmental testing.

4-Nitrophenyl beta-galactoside (pNP-Gal) is a chromogenic substrate commonly used in enzymatic assays to detect and quantify the presence of beta-galactosidase. It is hydrolyzed by the enzyme to release 4-nitrophenol, which produces a yellow color that can be measured spectrophotometrically. Similarly, 4-Nitrophenyl beta-glucoside (pNP-Glc) can be used to assay beta-glucosidase activity. 4-Nitrophenyl beta-globotrioside (pNP-Glo) is a similar compound that has been designed as a substrate for glycosidases that cleave terminal beta-galactosyl-(1->3)-N-acetylgalactosamine residues. In these assays, the release of 4-nitrophenol can be measured to determine the activity of the enzyme under study. The use of chromogenic substrates like pNP-Glo allows for the rapid and reliable detection of glycosidase activity in a variety of biological samples.

Modified naphtol AS substrate used in histochemical and cytochemical staining

4-Nitrophenyl-myo-inositol-1-yl phosphate is a highly specific chromogenic substrate used for the detection, quantification, and characterization of phosphoinositide phosphatases. When cleaved by the target enzyme, it generates a highly absorbing 4-nitrophenyl moiety, producing a yellow color that can be measured spectrophotometrically. This versatile substrate is valuable in examining phosphoinositide phosphatase activity, enzyme kinetics, substrate specificity, and inhibition studies in various cellular processes, including signal transduction, lipid metabolism, and membrane trafficking.