Autres

<p>a-Naphthyl phosphate disodium salt hydrate is a highly water-soluble chromogenic substrate used to determine phosphatase activity in research and analytical applications, particularly for staining and differentiating acid and alkaline phosphatases.</p>

<p>4-Nitrophenyl a-D-thiomannopyranoside is a chemiluminescence substrate that can be used to measure the activity of β-galactosidase, an enzyme involved in the hydrolysis of lactose. This product is available as a white to yellowish powder and has been shown to have high purity and quality. It also displays strong fluorescence when reacted with hydrogen peroxide and has been used for diagnostics tests such as staining, food testing, and enzyme substrate reactions.</p>

<p>2-Chloro-4-nitrophenyl-beta-D-galactopyranoside is a chromogenic substrate used to test enzyme activities. It is commonly used in molecular biology research for the detection and measurement of beta-galactosidase activity.</p>

<p>Chromogenic substrate for α-L-arabinofuranosidase yielding a yellow solution upon cleavage.</p>

<p>Aldol® 470 beta-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-galactosidase activity. The colorless enzyme substrate is cleaved by lactose-utilizing bacteria grown in liquid or solid media, yielding yellow coloration and green fluorescence. Aldol® 470 beta-D-galactopyranoside can also be used in beta-galactosidase gene reporter assays or immunoassay applications (ELISA). Aldol® 470 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>4-Nitrophenyl 2-acetamido-4-O-(2-acetamido-2-deoxy-b-D-glucopyranosyl)-2-deoxy-a-D-galactopyranoside is an enzyme substrate that is used in the detection of phenylalanine, tyrosine, and tryptophan. It is also used for environmental testing, bioluminescence, culture media, chemiluminescence, fluorogenic substrate, food testing, diagnostics and other applications.</p>

<p>4-Nitrophenyl 2-O-(beta-D-glucopyranosyl)-beta-D-glucopyranoside is a chromogenic substrate for beta-glucosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 2-O-(beta-D-glucopyranosyl)-beta-D-glucopyranoside is used in different applications such as the Cellulose degradation studies, biofuel research</p>

<p>Aldol® 455 alpha-D-glucopyranoside is a high quality, environmentally tested, staining, and ligand for bioluminescent assays. It has been shown to be a chromogenic substrate for diagnostics and a fluorogenic substrate for enzymes such as alpha-glucosidase, producing a yellow/green-orange color. Aldol® 455 alpha-D-glucopyranoside is also used in culture media and food testing.</p>

<p>4-Nitrophenyl alpha-D-glucuronide is a chromogenic substrate widely used for detecting and quantifying the activity of alpha glucuronidase enzyme. Upon cleavage by the enzyme, it releases the yellow-colored 4-nitrophenol, whose absorbance can be monitored spectrophotometrically. This substrate is routinely employed in biochemistry and molecular biology applications, such as enzyme assays, enzyme kinetics studies, and characterization of recombinant enzymes. As a non-toxic and easy-to-use reagent, 4-Nitrophenyl alpha-D-glucuronide provides a convenient and sensitive method for studying alpha-glucuronidase activity in various biological samples, including cell extracts, tissue homogenates, and purified enzyme preparations.</p>

<p>4-Nitrophenyl beta-D-glucopyranoside-6-phosphate is a chromogenic substrate for glucose-6-phosphatase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl beta-D-glucopyranoside-6-phosphate is used in different applications such as the Glycogen storage disease diagnosis (e.g., von Gierke disease)</p>

<p>4-Nitrophenyl 2,3,4,6-tetra-O-acetyl-a-D-thiomannopyranoside is a pNP substrate that serves as a chromogenic substrate for enzyme assays. It has been tested for the detection of a-D-mannosidase activity in a variety of biological samples, including cell lysates, body fluids, and tissue homogenates.</p>

<p>4-Nitrophenyl 2-acetamido-3-O-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)-2-deoxy-alpha-D-galactopyranoside is a chromogenic substrate for alpha-galactosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 2-acetamido-3-O-(2-acetamido-2-deoxy-beta-D-glucopyranosyl)-2-deoxy-alpha-D-galactopyranoside is used in different applications such as the Fabry disease diagnosis, glycolipid metabolism studies</p>

<p>6-Chloro-1H-indol-3-yl hexadecanoate is a chromogenic substrate for an enzyme that oxidizes it to produce a blue color. It can be used in the diagnosis of bacterial infections, such as tuberculosis and leprosy. 6CHIH is a ligand for various enzymes, including lactate dehydrogenase, which catalyzes the conversion of pyruvate to lactic acid. This compound reacts with oxygen to produce light in the presence of a catalyst. 6CHIH is also used as a fluorescent tag for protein labeling and detection.<br>6CHIH has been shown to be effective in detecting bacteria in food products and water samples using bioluminescence techniques. The high purity and quality of this compound make it suitable for use in diagnostics, culture media, and environmental testing.</p>

<p>Aldol 458 acetate is a fluoroquinolone antibiotic. It is an enzyme substrate that can be used in the detection of β-galactosidase, alkaline phosphatase, and other enzymes. Aldol 458 acetate can be used for the detection of β-galactosidase by chemiluminescence or bioluminescence. It has been shown to have high purity and high quality as well as being a ligand that can be conjugated with a fluorescent dye to provide a fluorescent substrate. Aldol 458 acetate can also be used in culture media for the growth of bacteria. It has been shown to have strong inhibitory effects on Escherichia coli, Bacillus subtilis, Pseudomonas aeruginosa, and Staphylococcus aureus.</p>

<p>Chromogenic substrate for phosphatase</p>

<p>Chlorophenol red beta-D-galactopyranoside sodium salt is a water soluble, colorless salt that is used as a substrate for the reduction of contaminants in soil and water. It can be used to measure the activity of microorganisms in the environment and has been shown to inhibit growth of certain bacteria, such as methylotrophic bacteria. Chlorophenol red beta-D-galactopyranoside sodium salt is also used in biological treatment systems. This product can be mixed with other substances in order to regulate the microbial population and reduce nutrients.</p>

<p>2-Naphthyl-beta-D-glucuronide sodium salt is an enzyme substrate to detect beta-glucuronidase. The enzyme cleaves the glycosidic bond in 2-naphthyl-beta-D-glucuronide to release 2-naphtol and glucuronic acid. Beta-glucuronidase activity assays are important in many fields such as cancer research, as the enzyme is expressed in some tumors and its activity can be targeted for cancer therapy. The enzyme has also an important role in drug metabolism as it recognizes and binds to drug glucuronide conjugate (as 2-naphthyl-beta-D-glucuronide) facilitating its hydrolysis to release the active part. 2-Naphthyl-beta-D-glucuronide sodium salt has a higher solubility as it is in its salt form.</p>

<p>5-Bromo-4-chloro-1-methyl-3-indolyl-α-D-glucopyranoside is a fluorescent substrate used for the detection of α-glucuronidase.</p>

<p>Chromogenic substrate for neuraminidase yielding a blue precipitate upon cleavage.</p>

<p>5-Bromo-4-chloro-3-indolyl β-L-fucopyranoside (X-Fuc) is a chromogenic substrate specifically designed for the identification and detection of fucosidase enzyme activity. Upon cleavage by fucosidase, it generates a blue-colored 5-Bromo-4-chloro-3-hydroxy-indole precipitate, which can be easily observed under a microscope or spectrophotometer. This feature makes X-Fuc an invaluable tool across various applications, such as biochemical analysis, histochemical staining, cell biology, and enzyme kinetic assays. By providing a fast, sensitive, and reliable readout for fucosidase activity, 5-Bromo-4-chloro-3-indolyl β-L-fucopyranoside facilitates essential research in the understanding of biological processes and the development of therapeutic interventions.</p>

<p>5-Bromo-4-chloro-3-indolyl a-D-mannopyranoside is a chromogenic substrate used to detect the specific enzymatic activity of alpha-mannosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl a-D-mannopyranoside is used in alpha-mannosidosis diagnosis and other lysosomal storage disorder.</p>

<p>5-Bromo-4-chloro-3-indolyl b-D-xylopyranoside is an enzyme substrate. This chromogenic substrate is used for beta-D-xylosidase enzyme. In culture media, 5-Bromo-4-chloro-3-indolyl b-D-xylopyranoside is utilised for identification of bacteria such as Klebsiella and Enterobacter.</p>

<p>(5α)-17-(3-Pyridinyl)androst-16-en-3-one is a natural metabolite of testosterone that is produced in the liver. It has been identified as an impurity in API, which can be found in drugs used for the treatment of high blood pressure and prostate cancer. (5α)-17-(3-Pyridinyl)androst-16-en-3-one is not active as a drug itself, but it can be used as a standard to study the metabolism of testosterone and other steroids.</p>

<p>Chromogenic β-glucosaminidase substrate yielding a yellow solution upon cleavage, used particularly in yeasts and molds.</p>

<p>Chromogenic substrate for α-galactosidase yielding a blue precipitate. Used for species differentiation within the family Enterobacteriaceae and differentiation of Bifido bacteria species from Lactobacillus species.</p>

<p>2-Chloro-4-nitrophenyl 4-O-b-D-galactopyranosylmaltoside, also known as Gal-G2-CNP, is a high quality, water soluble, chromogenic substrate that is used as an enzyme substrate and can be used in bioluminescence and chemiluminescence assays. This product is designed for use in diagnostics, culture media, food testing, environmental testing, and other research applications. 2-Chloro-4-nitrophenyl 4-O-b-D-galactopyranosylmaltoside has been tested in chromogenic assays with alpha-amylases.</p>

<p>Chromogenic substrate for α-glucosidase</p>

<p>5-Bromo-4-chloro-3-indoxyl-alpha-D-N-acetylneuraminic acid, cyclohexylammonium salt is a chemiluminescent substrate that provides a significant increase in light emission. It has a CAS number of 59820-92-0 and is supplied in high purity and high quality. This product is used for the detection of various biological reactions, including diagnostics, enzyme substrates, food testing, and more.</p>

<p>5-Bromo-4-chloro-3-indolyl b-D-fucopyranoside is a chromogenic substrate used is for the enzyme beta-fucosidase.</p>

<p>6-Bromo-2-naphthyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a chromogenic substrate used for the detection of chitinase activity. Upon hydrolysis by chitinase, it releases a yellow-brown color, which is easily detected by spectrophotometry. This substrate has been used to study chitinases in various organisms, including bacteria, fungi, and insects. It can also be used to quantitate chitinase activity in various biological samples.</p>

<p>4-(Hydroxymethyl)phenyl-alpha-D-galactopyranoside tetraacetate is a substrate for enzymes that catalyze the hydroxylation of carbohydrates. It can be used as a substrate for peroxidase and beta-glucosidase, which are important in the production of natural products such as resveratrol, catechins, and lignin. 4-(Hydroxymethyl)phenyl-alpha-D-galactopyranoside tetraacetate is also a chromogenic substrate for chemiluminescence reactions. The product is available as a powder and can be used in diagnostic kits to detect the presence of enzymes or other substances in food or environmental samples.</p>

<p>X-Gal is a chromogenic substrate for β-galactosidase, yielding a blue precipitate. X-Gal is an indicator for coliforms in culture media, as well as for the detection of coliforms (E. coli) in municipal water supplies and food products. X-Gal can be used in conjunction with the inducer IPTG, which binds and inhibits the lac repressor thus inducing β-galactosidase expression. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.<br>Upon hydrolysis, X-Gal yields a localized, insoluble, blue precipitate, making it exceptionally useful in blotting, immunocytochemical, and ELISA assays. 5-Bromo-4-chloro-3-indoxyl-beta-D-galactopyranoside (X-beta-D-galactoside, X-Gal) is a chromogenic indicator for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid media or on agar plates and yields a blue-green colored precipitate.<br>X-Gal can also be used as a detecting agent for beta-galactosidase activity in reporter gene assays or for identification of positive transformants on agar plates when using the &amp;alpha;-complementation approach. Color formation is due to dimerization and oxidation of 5-bromo-4-chloro-3-hydroxyindole which results from enzymatic cleavage of X-Gal. The reaction proceeds only in the presence of oxygen, i.e. in aerobic cultures. Well detectable color bacterial colonies or liquid cultures is obtained within 16-48 h. In the presence of purified beta-galactosidase, color develops within minutes to a few hours.</p>

<p>Aldol® 515 nonanoate hydrochloride is a chromogenic and fluorogenic enzyme substrate for detection of lipase activity. The colorless product is cleaved by C9 esterases produced by bacteria in liquid or solid media, yielding a red color and red fluorescence when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 515 nonanoate can be used under aerobic and anaerobic conditions. Well detectable color/fluorescence of bacterial colonies or liquid cultures is obtained within 24-48 h. In the presence of a purified lipase, color and fluorescence develops within minutes to few hours.</p>

<p>Aldol® 470 beta-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of ?-galactosidase activity. The colorless enzyme substrate is cleaved by lactose-utilizing bacteria grown in liquid or solid media, yielding yellow coloration and green fluorescence. Aldol® 470 beta-D-galactopyranoside can also be used in ?-galactosidase gene reporter assays or immunoassay applications (ELISA). Aldol® 470 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>Aldol® 458 beta-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-galactosidase activity. The colorless enzyme substrate is cleaved by lactose utilizing bacteria grown in liquid or solid media, yielding yellow coloration and green fluorescence. Aldol® 458 beta-D-galactopyranoside can also be used in beta-galactosidase gene reporter assays or immunoassay applications (ELISA). Aldol® 458 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>Chromogenic substrate for beta-galactofuranosidase</p>

<p>Water soluble, chromogenic substrate for visualizing alkaline phosphatase activity. Produces a blueinsoluble end product that is detected visually when used together with nitroblue tetrazolium. The substrate system is versatile and functions in a variety of applications, including Northern, Southern, and Western blotting, in situhybridization, ELISAs and immunohistochemistry.</p>

<p>Chromogenic beta-maltosidase substrate</p>

<p>Aldol® 470 choline phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatidyl-choline specific phospholipase C (PC-PLC, EC 3.1.4.3) activity. The colorless product is cleaved by PC-PLC positive microorganisms in liquid or solid media, yielding a yellow color and green fluorescence. Aldol® 470 choline phosphate therefore, enables the detection and identification of PC-PLC positive pathogenic bacteria, such as, Clostridium perfringens, Pseudomonas aeruginosa, Helicobacter pylori, Legionella pneumophila, Bacillus cereus, Bacillus anthracis, Listeria monocytogenes, and others. Aldol® 470 choline phosphate can be used under aerobic and anaerobic conditions.</p>

<p>4-Nitrophenyl O-(N-acetyl-a-neuraminosyl)-(2,3)-O-b-D-galactopyranosyl-(1,3)-2-acetamido-2-deoxy-a-D-galactopyran oxide is a fluorogenic substrate that is used in the detection of glycosidases. It has been shown to be an excellent ligand for the enzyme beta galactosidase, which cleaves the glycan substrate 4NPOBGPA into 4NP and O-(N acetyl) neuraminic acid. 4NPOBGPA can be used as a chromogenic substrate for detecting beta galactosidase activity and can also be conjugated to other fluorescent molecules. This product is high purity and high quality.</p>

<p>Aldol® 515 acetate is a chromogenic and fluorogenic substrate, used for detection of esterase activity. The colorless product is cleaved by purified esterase, or esterase secreted by microorganisms in liquid or solid media, yielding a red color and red fluorescence signal when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 515 acetate can be used under aerobic and anaerobic conditions. Well detectable color/fluorescence of bacterial colonies or liquid cultures is obtained within 16-48 h. In the presence of a purified esterase, color and fluorescence develops within minutes to few hours.</p>

<p>Chromogenic substrate for esterase with C8 activity yielding a blue precipitate upon cleavage.</p>

<p>Chromogenic substrate targeting beta- galactosidase and whose color is dependent on the metal chelating.Alizarin-2-beta-D-galactopyranoside is a chromogenic substrate for enzymes based on alizarin and has been reported for the detection of bacterial beta-galactosidase by researchers at the University of Northumbria and the Freeman Hospital, Newcastle upon Tyne in the UK (James, 2000).</p>

<p>Aldol® 470 acetate is a Fluorogenic substrate that is used in enzymatic reactions to produce a fluorescent product. Aldol® 470 acetate can be used as a ligand or a chromogenic substrate. This product is also used in diagnostic tests, such as conjugates and chemiluminescence, to detect the presence of certain enzymes or microorganisms. It can be used in culture media to measure the amount of certain enzymes, such as phosphatases or proteases, and has been shown to have high purity and quality. This product has CAS No. 1318785-37-3, 1318785-38-4, 1318785-39-5, 1318785-40-6 and 1318785-41-7.</p>

<p>Aldol® 584 is a fluorogenic substrate that can be used for enzymatic and chemical assays. It is an important component of the Cymit Quimica Aldol Reagent Kit and other diagnostic kits. Aldol® 584 is used in immunoassays, such as ELISA, to measure the activity of serine proteases, including cathepsin D, elastase, and leukocyte elastase. This product has a CAS number of 2484873-11-0.</p>

<p>5-Bromo-4-chloro-3-indoxyl thymidine (BCIT) is a chromogenic substrate that reacts with the enzyme β-galactosidase, which cleaves the BCIT to form a bromochloroindoxyl (BCI) and an indoxyl phosphate. The BCI is then detected by chemiluminescence or fluorescence. This product is used in food testing, diagnostics, environmental testing, and staining. It has been shown to be a good substrate for β-galactosidase from different sources such as calf thymus, bacteria, fungi, and plants.</p>

<p>Aldol® 495 phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatase activity. The colorless enzyme substrate is cleaved by acid and alkaline phosphatases secreted by bacteria in liquid or solid media, yielding orange color and green fluorescence. Aldol® 495 phosphate can also be used in Western blots and other immunoassay applications with phosphatase-coupled secondary antibodies. Aldol® 495 phosphate can be used under aerobic and anaerobic conditions.</p>

<p>Substrate for feruloyl esterases</p>

<p>Also known as Lapis-caprylate; targeting esterase with C8 activity; Blue color</p>

<p>Chromogenic substrate for beta-glucuronidase (GUS) yielding blue precipitate upon cleavage. Used for identification of beta-glucuronidase. X-beta-D-glucuronide has been reported as chromogenic substrate in a number of chromogenic E. coli/ coliform culture media. About 95% of common E. coli possess beta-D-glucuronidase activity. Such chromogenic culture media are used for detection and enumeration of E. coli in food samples such as meat, dairy products and shellfish and have clinical applications in the assessment of urinary infections. It has gained international acceptance as highly accurate test for the presence of E. coli in drinking water samples. It is also used for the detection of the expression of GUS (E. coli beta-glucuronidase) gene fusion marker constructs in plants. The sodium salt has advantages over the cyclohexylammonium salt as being non-electrostatic and possessing a naturally-occurring cation.</p>

<p>4-Nitrophenyl 2-acetamido-3-O-[2-O-a-L-fucopyranosyl-b-D-galactopyranosyl)-2-deoxyb-D-galactopyranoside is a water soluble, fluorescent probe that can be used for detecting the presence of β-(1→4) -linked galactosaminoglycans. The dye is a substrate for enzymes such as α--mannosidase, β--glucuronidase and β--galactosidase. The dye is also an excellent ligand for lectins such as concanavalin A and wheat germ agglutinin. This product is a diagnostic reagent that has been shown to have high purity and quality. It has CAS No. 93496-53-8 and can be used in food testing, bioluminescence, environmental testing, or high purity applications.</p>

<p>For the detection of beta-galactosidase activity</p>

<p>Please enquire for more information about 3-Indoxyl choline phosphate including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>Chromogenic substrate for β-D-glucosidase yielding a magenta precipitate. Differentiation of Salmonella spp. (negative) from other Enterobacteriaceae.</p>

<p>6-Chloro-1H-indol-3-yl octanoate is a fluorogenic substrate that can be used to detect enzymes such as phosphatases, lipases, esterases, and proteases. 6-Chloro-1H-indol-3-yl octanoate is a ligand for metal ions such as Cu(II), Ni(II) and Zn(II). This product has high purity and quality. It is an enzyme substrate for many enzymes such as phosphatases, lipases, esterases, and proteases. This product can be used in diagnostics of various substances including food testing, environmental testing and staining. 6CIO is a chromogenic substrate that can be used to detect bioluminescence reactions.<br>6CIO is CAS No. 159954-35-5.</p>

<p>5-Bromo-3-indoxyl phosphate, disodium salt is an enzyme substrate that is used for environmental testing, bioluminescence, culture media, chemiluminescence, fluorogenic substrate and food testing. It is also used as a ligand for diagnostic purposes. 5-Bromo-3-indoxyl phosphate, disodium salt has CAS number 16036-59-2 and it is soluble in water. This product has high quality and purity with a conjugate of 5% phenol red. 5-Bromo-3-indoxyl phosphate, disodium salt reacts with the enzyme horseradish peroxidase to produce a red color. This product can be used to detect hydrogen peroxide in the presence of an oxidizing agent such as hydrogen peroxide or potassium permanganate. The reaction produces a blue color when the solution is viewed in daylight or under ultraviolet light.</p>

<p>5-Bromo-4-chloro-3-indoxyl myristate is a chemiluminescent substrate that can be used in the detection of peroxide. It reacts with hydrogen peroxide to emit light, which can be detected using a luminometer. 5-Bromo-4-chloro-3-indoxyl myristate is also used for the detection of bacteria, such as Staphylococcus aureus and Escherichia coli, by staining. The compound is also used for diagnostic tests to identify diseases such as tuberculosis. 5-Bromo-4-chloro-3 indoxyl myristate is soluble in organic solvents and water, not toxic, and has a high purity level.</p>

<p>4-Nitrophenyl 6-O-(alpha-D-mannopyranosyl)-alpha-D-mannopyranoside is a chromogenic substrate for alpha-mannosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 6-O-(alpha-D-mannopyranosyl)-alpha-D-mannopyranoside is used in different applications such as the Lysosomal storage disorder diagnosis (e.g., alpha-mannosidosis)</p>

<p>8-Hydroxyquinoline-beta-D-glucuronic acid, sodium salt is a fluorogenic substrate that can be used for enzymatic detection of peroxidase. The substrate is available in a variety of forms, including powder and liquid. This product has been tested for environmental and bioluminescence activity, as well as food safety. It has also been shown to be a useful ligand in immunoassays.</p>

<p>Aldol® 515 palmitate is a chromogenic substrate that is used in food testing, diagnostics, and other applications. It has a high purity and is conjugated to various ligands for specific applications. Aldol® 515 palmitate is an excellent chemiluminescent substrate for the detection of ATP and other nucleotides. It also has high bioluminescence activity for the detection of adenosine-5'-triphosphate (ATP) or luciferin as well as enzyme substrates to measure β-galactosidase or β-glucuronidase activity.</p>

<p>Chromogenic substrate for alpha-L-fucosidase</p>

<p>4-Nitrophenyl 2-acetamido-6-O-(2-acetamido-2-deoxy-b-D-glucopyranosyl)-3-O-(b-D-galactopyranosyl)-2-deoxy-a-D-galactopyranoside is a specific chromogenic substrate designed for the highly sensitive and accurate detection of glycosidase enzyme activity. Glycosidases are responsible for the cleavage of glycosidic bonds between saccharide units in various carbohydrates. The hydrolysis of this substrate by glycosidase enzymes produces 4-nitrophenol, a yellow-colored compound that can be easily monitored through spectrophotometric measurements due to its strong absorbance at 405 nm. This chromogenic substrate is widely used in the analysis of glycosidase activity in various biological samples, including bacterial, fungal, and plant tissues, as well as in environmental samples and biotechnological applications.</p>

<p>5-Bromo-4-chloro-3-indoxyl-alpha-D-N-acetylneuraminic acid, cyclohexylammonium salt is a Fluorogenic substrate used for the detection of an enzyme that converts sialic acid to fluorescein. This product has a high quality and purity with a CAS number. It is used in Culture media as a conjugate, Enzyme substrate, Food testing, High purity, Diagnostics, ligand, Chromogenic substrate, Environmental testing. The product has been tested and found to be safe for use with animals and humans.</p>

<p>6-Bromo-2-naphthyl alpha-D-glucopyranoside is a chromogenic substrate used to detect alpha-glucosidase enzyme activity. When cleaved by the enzyme alpha-glucosidase, it releases 6-bromo-2-naphthol, which can be coupled with diazonium salts (for example 4'-amino-2,3'-dimethylazobenzene, also known as Fast Garnet GBC) to form a colored precipitate. 6-Bromo-2-naphthyl alpha-D-glucopyranoside is used in biochemical assays, histochemistry, and diagnostic tests for conditions like lysosomal storage disorders (e.g. Pompe disease) and carbohydrate metabolism studies.</p>

<p>3,3'-Diaminobenzidine (DAB) is an organic compound derived from benzidine. DAB is water-soluble as the tetrahydrochloride salt and is used in immunohistochemical staining of nucleic acids and proteins. Within research in Alzheimer's disease, Aβ protein amyloid plaques are targeted by a primary antibody, and subsequently by a secondary antibody, which is conjugated with a peroxidase enzyme. This will bind DAB as a substrate and oxidize it, producing an easily observable brown colour. 3,3’-Diaminobenzidine tetrahydrochloride hydrate is also available as the tetrahydrochloride salt and as the free base.</p>

<p>Please enquire for more information about 5-Bromo-6-chloro-3-indoxyl choline phosphate including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>For detection of beta-galactosidase activity. <br>The colors of the chromogen can be adjusted by incorporating different functional groups, e.g. cyano or nitro group, which yields purple or black green colonies with beta-galactosidase positive strain.</p>

<p>Modified naphtol AS substrate used in histochemical and cytochemical staining</p>

<p>5-Bromo-4-chloro-3-indolyl b-D-lactopyranoside is a chromogenic substrate used to detect the specific enzymatic activity of beta-galactosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl b-D-lactopyranoside is used in lactose intolerance screening.</p>

<p>6-Chloro-3-indoxyl-beta-D-galactopyranoside is a chromogenic substrate for beta-galactosidase that yields a salmon or rose-coloured precipitate and hence is also known as Salmon-Gal. It can be used in conjunction with isopropyl-beta-D-thiogalactopyranoside (IPTG) as an alternative to the chromogenic substrate 5-bromo-4-chloro-3-indolyl beta-D-galactopyranoside (X-Gal) for detection of beta-galactosidase activity in bacterial colonies in colorimetric assays. Where beta-galactosidase expressed from a reporter gene in molecular biology experiments, recombinant colonies on growth plates are salmon-coloured while non-recombinants appear as white. Use of nitroblue tetrazolium salt (NBT) with Salmon-Gal may provide faster results, yielding a clear, purple precipitate, than for Salmon-Gal alone. In conjunction with 5-bromo-4-chloro-3-indolyl-beta-D-glucoside (X-Glc), Salmon-Gal is useful for simultaneous detection of GUS and Lac activities in the same medium.</p>

<p>A substrate for alkaline phosphatase yielding a blue insoluble product upon cleavage. Colour can be further enhanced when used together with Nitroblue tetrazolium. The substrate system is versatile and has functions in a variety of applications, including Northern, Southern, and Western blotting, in situ hybridization, ELISAs, microarray and immunohistochemistry. The micronized structure enhances solubility.</p>

<p>Chromogenic β-xylosidase substrate yielding a yellow solution upon cleavage.</p>

<p>Naphthol AS-OL is a widely used chromogenic enzyme substrate that forms a colored product upon reaction with specific enzymes. It is commonly employed in histochemical and cytochemical staining techniques to visualize enzyme activity in cells and tissues. Naphthol AS-OL is particularly useful for detecting the presence of enzymes such as alkaline phosphatase, acid phosphatase, and esterases. The resulting colored product can be easily observed under a microscope, allowing researchers to study the distribution and localization of these enzymes in various biological samples.</p>

<p>6-Chloro-3-indolyl butyrate is a chromogenic substrate for lipases. Use in lipid digestion and metabolism studies, it produces a salmon-pink color when 6-chloro-3-indolyl is released after enzymatic cleavage.</p>

<p>Chromogenic β-galactosidase substrate yielding a yellow solution upon cleavage. Can be used for the detection of coliforms due to their beta-galactosidase activity.</p>

<p>Chromogenic substrate for beta-galactosidase</p>

<p>4-Nitrophenyl 2-acetamido-2-deoxy-a-D-glucopyranoside is a chromogenic pNP substrate specifically designed for the analysis of N-acetylglucosaminidase activity. Upon enzyme action, the substrate releases 4-nitrophenol, a yellow compound that can be detected by spectrophotometric methods, providing a reliable and sensitive means of quantifying enzyme activity. This versatile substrate is widely used in biochemical studies, disease diagnostics, and the enzyme production industry.</p>

<p>4-Nitrophenyl a-D-mannopyranoside is a chromogenic pNP enzyme substrate that enables the detection and quantification of a-D-mannosidase activity in various biological samples through a colorimetric assay. The hydrolysis of the substrate by the enzyme releases 4-nitrophenol, a yellow compound whose absorption can be measured spectrophotometrically. Applications for this substrate span biomedical research, disease diagnostics, and industrial enzyme production.</p>

<p>Aldol® 514 inositol-phosphate is a chromogenic enzyme substrate used for detection of phosphatidylinositol specific phospholipase C (PI-PLC, EC 4.6.1.13). The colorless enzyme substrate is cleaved by PI-PLC positive microorganisms, yielding a red-orange color. The substrate thus enables the detection and identification of various bacteria, such as, pathogenic strains of Listeria, Clostridium, Bacillus, and Staphylococcus. Aldol® 514 inositol-phosphate is suitable for use under aerobic and anaerobic conditions.</p>

<p>5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, sodium salt anhydrous is a potent inhibitor of histidine decarboxylase (HDC). It is used in the histochemical staining of dehydrogenase and effector proteins in urine samples. This compound has been shown to inhibit the growth of macroalgae and can be used as a selective agent for phylogenetic tree construction. 5BDG,Sr with its high specificity for HDC, also inhibits cell proliferation in plant cells and ovules.</p>

<p>4-Nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt is a chromogenic substrate for N-acetylglucosamine-6-sulfatase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt is used in different applications such as the Mucopolysaccharidosis IIID (Sanfilippo D) diagnosis</p>

<p>4-Nitrophenyl phosphate mono(2-amino-2-ethyl-1,3-propanediol) salt monohydrate is a chromogenic substrate that can be used to detect the presence of alkaline phosphatase. It is also used in food testing and diagnostics. 4NPPMD is a substrate for the enzyme alkaline phosphatase, which has been shown to play a role in the inflammatory response. The production of 4NPPMD by bacteria in the environment can be detected with chemiluminescence or bioluminescence. This product has been shown to react with both horseradish peroxidase and alkaline phosphatase, as well as other enzymes that are involved in metabolic pathways. This substrate also has high purity and fluorescence properties.</p>

<p>Chromogenic substrate yielding a soluble yellow chromophor for the colorimetric assay of α-L-fucosidase, especially in human serum.</p>

<p>2-Chloro-4-nitrophenyl alpha-D-mannopyranoside is a substrate used to measure the alpha-mannosidase activity. This enzyme plays a crucial role in the lysosomal degradation of asparagine-linked carbohydrate cores of glycoproteinsis. The use of 2-Chloro-4-nitrophenyl alpha-D-mannopyranoside in diagnostic assays is important as a deficiency in alpha-mannosidase leads to the accumulation of mannose-rich oligosaccharides in lysosomes, causing a range of health problems collectively known as alpha-mannosidosis.</p>

<p>Aldol® 518 myo-inositol phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatidylinositol specific phospholipase C (PI-PLC, EC 4.6.1.13) activity. The colorless enzyme substrate is cleaved by PI-PLC positive microorganisms grown in liquid or solid media, yielding intense red color. In combination with Aldol® 355 fluorescence enhancer, red fluorescence can also be obtained. Aldol® 518 myo-inositol phosphate thus enables the detection and identification of various bacteria, such as, pathogenic strains of Listeria, Clostridium, Bacillus, and Staphylococcus species. Aldol® 518 myo-inositol phosphate is suitable for use under aerobic and anaerobic conditions.</p>