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5-Bromo-3-indolyl a-D-mannopyranoside is a chromogenic substrate that is used as an enzyme substrate for various spectrophotometric, fluorometric, or chemiluminescent assays. The 5-bromo group of the dye reacts with the active site of an enzyme to form a product that can be measured by spectrophotometry. This product can also be used as a fluorescent indicator in the presence of luciferase and ATP. 5-Bromo-3-indolyl a-D-mannopyranoside is also used to estimate bacterial counts during culture media preparation and it has been shown to inhibit the growth of Gram negative bacteria such as E. coli and Pseudomonas aeruginosa. This compound does not contain any heavy metals or toxic substances and it is highly soluble in water. It has been found to have no adverse effects on humans and other animals at concentrations up to 100

4-Nitrophenyl trimethylacetate is a cytosolic proteinase inhibitor. It binds to the active site of trypsin and other enzymes that hydrolyze peptide bonds, thereby inhibiting the activity of these enzymes. 4-Nitrophenyl trimethylacetate has been shown to inhibit soybean trypsin, which has led to its use as a model system for studying enzyme inhibition. The binding site on 4-Nitrophenyl trimethylacetate is competitive with respect to substrate binding but not with respect to the enzyme's catalytic triad. This inhibitor also has a high buffer capacity and can be used in low pH systems.

Jalapeno green-Gal (Jalapeno green-beta-D-galactopyranoside) is a chromogenic indicator for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid media or on agar plates and yields a Jalapeno green colored precipitate. Jalapeno green-Gal can also be used as detecting agent for beta-galactosidase activity in reporter gene assays or for identification of positive transformants on agar plates when using the α-complementation approach.

4-Nitrophenyl 4,6-O-benzylidene-b-D-glucopyranoside is a fluorescent compound that is used in diagnostics and research. The compound is an enzyme substrate, a ligand for metal ions, and a chromogenic substrate. It can be used to test cultures of bacteria in culture media, as well as for food testing and environmental testing. 4-Nitrophenyl 4,6-O-benzylidene-b-D-glucopyranoside has high purity and quality due to its high fluorescence intensity. This product has been shown to be highly stable in various conditions and may be used in conjugates with other compounds.

4-Nitrophenyl b-D-cellohexaoside is a substrate for many enzymes and can be used to detect the presence of these enzymes in biological samples. It can also be used to detect the presence of bacteria, fungi, parasites, and viruses. 4-Nitrophenyl b-D-cellohexaoside is an excellent substrate for enzyme reactions that produce a chromogenic or fluorogenic product. This product is often conjugated with other compounds such as antibodies or antigens to form an immunoassay. 4-Nitrophenyl b-D-cellohexaoside is made from a high purity chemical by a process called nitration with sodium nitrate and sulfuric acid.

4-Nitrophenyl 2-O-(2,3,4,6-tetra-O-acetyl-a-D-mannopyranosyl)-a-D-mannopyranoside is a fluorogenic substrate that is used as a diagnostic tool and can be used to detect the presence of β--glucosidase in the gut. It is also used as a conjugate with enzymes such as peroxidase or alkaline phosphatase for use in enzyme catalyzed reactions. The product has been shown to be active against a variety of bacteria, including Staphylococcus aureus and Escherichia coli. This product has been shown to produce chemiluminescence when incubated with horseradish peroxidase and hydrogen peroxide. This product is light sensitive and should be stored at room temperature away from direct sunlight.

2-Nitrophenyl 2-acetamido-2-deoxy-b-D-galactopyranoside is a carboxymethyl derivative of the natural substrate of β-galactosidase, which is used as a substrate for the production of recombinant proteins in the corynebacterium glutamicum. The enzyme β-galactosidase hydrolyzes this substrate to produce an acid molecule and a sugar molecule. This product can be used in biotechnological processes for the production of recombinant proteins or other bioactive molecules. It can also be used in the isolation of nucleic acid from bacteria or fungi, or to study bacterial membrane transport systems. 2NPGAG can be expressed as a recombinant protein with expression vectors such as pET21a and pET24a. It has been shown that this product inhibits transport through membranes by binding to cellulose, which may be useful for studies on cellulose biosynthesis

4-Nitrophenyl 2-acetamido-3-O-(3,4,6-tri-O-acetyl-2-deoxy-2-phthalimido-b-D-glucopyranosyl)-2-deoxy--4,6--O--4--methoxybenzylidene--a--D--galactopyranoside is a chemiluminescent substrate for the enzyme beta galactosidase. It reacts with this enzyme to form 4 nitrophenol and acetamide. 4 Nitrophenyl 2 acetamido 3 O (3,4,6 tri O acetyl 2 deoxy 2 phthalimido b D glucopyranosyl) 2 deoxy 4 6 O 4 methoxybenzylidene a D galactopyranoside is used as a chromogenic substrate in diagnostics and as an environmental testing agent. It can be used as an imaging

4-Nitrophenyl 6-O-DMT-2,3,4-tri-O-levulinoyl-a-D-mannopyranoside is a fluorogenic substrate that can be used for the detection of DMT (dihydroxyacetone) in food products. It has been shown to be an excellent alternative to the more commonly used 4,5′,6,7,8,-Tetrabromoindoxyl 3′,5′,6′,-tetraacetic acid (TBA). This product is also used as a chromogenic substrate for the determination of phenols in environmental testing and as a ligand conjugate for immunohistochemistry staining. The purity of this product is guaranteed and it has been tested extensively.

5-Bromo-3-indoxyl-beta-D-galactopyranoside (5BIG) is a fluorogenic substrate that has been shown to be an effective enzyme substrate. It is used in the chemiluminescence, bioluminescence, and fluorescence detection of enzymes. 5BIG is also a chromogenic substrate for nucleic acid staining and can be used in food testing as well as diagnostics. 5BIG is highly pure and high quality with a CAS number of 1301706-59-1.

5-Bromo-4-chloro-3-indoxyl-alpha-D-xylopyranoside is an enzyme substrate that can be used in the detection of β-galactosidase activity. This product has been shown to be a good ligand for various xanthine oxidoreductases, including xanthine dehydrogenase and uricase. 5BBIX is also a chromogenic substrate for horseradish peroxidase and can be used to detect hydrogen peroxide in the presence of osmium tetroxide or potassium permanganate. 5BBIX has been extensively tested for use in diagnostics, food, and environmental testing.

Modified naphtol AS substrate used in histochemical and cytochemical staining

1-Naphthyl-alpha-D-mannopyranoside is a chromogenic substrate. It is used in diagnostics and as a reagent for enzymatic analysis of alpha-mannosidase activity in culture media, food testing, and environmental testing.

4-Nitrophenyl alpha-D-maltotetraoside is a chromogenic substrate used for the detection of alpha-glucosidase and alpha-amylase activity. The four glucose units in the molecule are hydrolyzed by the enzyme, releasing 4-nitrophenol and allowing for easy colorimetric measurement of enzymatic activity

4-Nitrophenyl beta-galactoside (pNP-Gal) is a chromogenic substrate commonly used in enzymatic assays to detect and quantify the presence of beta-galactosidase. It is hydrolyzed by the enzyme to release 4-nitrophenol, which produces a yellow color that can be measured spectrophotometrically. Similarly, 4-Nitrophenyl beta-glucoside (pNP-Glc) can be used to assay beta-glucosidase activity. 4-Nitrophenyl beta-globotrioside (pNP-Glo) is a similar compound that has been designed as a substrate for glycosidases that cleave terminal beta-galactosyl-(1->3)-N-acetylgalactosamine residues. In these assays, the release of 4-nitrophenol can be measured to determine the activity of the enzyme under study. The use of chromogenic substrates like pNP-Glo allows for the rapid and reliable detection of glycosidase activity in a variety of biological samples.

4-Nitrophenyl-myo-inositol-1-yl phosphate is a highly specific chromogenic substrate used for the detection, quantification, and characterization of phosphoinositide phosphatases. When cleaved by the target enzyme, it generates a highly absorbing 4-nitrophenyl moiety, producing a yellow color that can be measured spectrophotometrically. This versatile substrate is valuable in examining phosphoinositide phosphatase activity, enzyme kinetics, substrate specificity, and inhibition studies in various cellular processes, including signal transduction, lipid metabolism, and membrane trafficking.