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5-Bromo-3-indoxyl-beta-D-galactopyranoside (5BIG) is a fluorogenic substrate that has been shown to be an effective enzyme substrate. It is used in the chemiluminescence, bioluminescence, and fluorescence detection of enzymes. 5BIG is also a chromogenic substrate for nucleic acid staining and can be used in food testing as well as diagnostics. 5BIG is highly pure and high quality with a CAS number of 1301706-59-1.

2-Nitrophenyl 2,3-di-O-acetyl-4-O-triethylsilyl-b-D-xylopyranoside is a chromogenic substrate for enzmatic assays to measure xylosidase activity. It is a derivative of xylose that can be hydrolyzed by xylosidase to release the yellow-colored 2-nitrophenyl group, which can be detected spectrophotometrically. By modifying the xylose molecule with acetyl and triethylsilyl groups, the enzyme substrate becomes more stable and resistant to degradation. This substrate is commonly used in the study of plant biomass degradation and in the detection of xylosidase activity in microorganisms.

2-Nitrophenyl 2-acetamido-2-deoxy-b-D-galactopyranoside is a carboxymethyl derivative of the natural substrate of β-galactosidase, which is used as a substrate for the production of recombinant proteins in the corynebacterium glutamicum. The enzyme β-galactosidase hydrolyzes this substrate to produce an acid molecule and a sugar molecule. This product can be used in biotechnological processes for the production of recombinant proteins or other bioactive molecules. It can also be used in the isolation of nucleic acid from bacteria or fungi, or to study bacterial membrane transport systems. 2NPGAG can be expressed as a recombinant protein with expression vectors such as pET21a and pET24a. It has been shown that this product inhibits transport through membranes by binding to cellulose, which may be useful for studies on cellulose biosynthesis

4-Nitrophenyl 3,4-O-isopropylidene-a-D-galactopyranoside is a chemiluminescent substrate for detecting beta-galactosidase activity in bacteria. This product is a high purity, high quality compound that can be used as a culture media and environmental testing agent. It has been shown to be effective in diagnostics, chromogenic substrate, and enzyme substrate reactions. 4NPPG has been shown to be a fluorogenic substrate for beta-galactosidase assay and can be used as food testing agent with conjugate.

Aldol® 455 is a substrate for the enzyme beta-galactosidase. It is used in food testing, staining, and environmental testing. Aldol® 455 has been shown to be a good substrate for fluorogenic and chromogenic substrates. It can be conjugated with an antibody or other ligand for use in immunoassays. Aldol® 455 is of high purity and high quality, making it suitable for use in diagnostics and as an enzyme substrate. This product can also be used as a culture media additive.

4-Nitrophenyl b-D-thiomannopyranoside acts as a substrate for β-mannosidase and thioglycoligase. Upon enzymatic cleavage, these substrates release 4-nitrophenol, a yellow chromophore that can be detected spectrophotometrically at 405 nm, facilitating quantitative measurements of enzymatic activity. Consequently, these substrates are widely used in biochemical research, clinical diagnostics, and drug discovery for evaluating the function of specific enzymes in various biological processes.

4-nitrophenyl 6-O-DMT-a-D-mannopyranoside is a fluorescent substrate for the enzyme beta-glucosidase. It reacts with an acyl group at the C2 position of the glycoside moiety, producing an aldehyde group at the C1 position and a hydroxymethylene group at the C4 position. This reaction is catalyzed by beta-glucosidase, which cleaves the glycosidic linkage in sucrose and other disaccharides. The product can be detected using luminescence methods, such as chemiluminescence or bioluminescence.
4-nitrophenyl 6-O-DMT-a-D-mannopyranoside is used to detect β glucosidase activity in bacteria. This substrate has been shown to be sensitive and specific for β glucosidase activity when combined with fluorescence detection technology

4-Nitrophenyl b-D-cellohexaoside is a substrate for many enzymes and can be used to detect the presence of these enzymes in biological samples. It can also be used to detect the presence of bacteria, fungi, parasites, and viruses. 4-Nitrophenyl b-D-cellohexaoside is an excellent substrate for enzyme reactions that produce a chromogenic or fluorogenic product. This product is often conjugated with other compounds such as antibodies or antigens to form an immunoassay. 4-Nitrophenyl b-D-cellohexaoside is made from a high purity chemical by a process called nitration with sodium nitrate and sulfuric acid.

4-Nitrophenyl 4,6-O-benzylidene-3-O-(2,3,4,6-tetra-O-acetyl-b-D-glucopyranosyl)-b-D-glucopyranoside is a high purity fluorescent reagent that can be used as a substrate for many enzymes. It is also used in environmental testing and to detect the presence of nitrophenols in food. This product has CAS number 545414.

4-Nitrophenyl 2-O-(α-D-glucopyranosyl)-α-D-glucopyranoside is a specialized chromogenic pNP substrate featuring dual glucose-linked moieties, designed for accurate detection and quantification of specific glycosidase enzymes. Upon cleavage by target enzymes, this substrate releases 4-Nitrophenol, which can be easily monitored spectrophotometrically as a yellow color change. This advanced substrate offers enhanced specificity and sensitivity in enzyme assays, facilitating cutting-edge research in biochemistry, enzymology, and other related fields.

Modified naphtol AS substrate used in histochemical and cytochemical staining

1-Naphthyl-alpha-D-mannopyranoside is a chromogenic substrate. It is used in diagnostics and as a reagent for enzymatic analysis of alpha-mannosidase activity in culture media, food testing, and environmental testing.

4-Nitrophenyl beta-galactoside (pNP-Gal) is a chromogenic substrate commonly used in enzymatic assays to detect and quantify the presence of beta-galactosidase. It is hydrolyzed by the enzyme to release 4-nitrophenol, which produces a yellow color that can be measured spectrophotometrically. Similarly, 4-Nitrophenyl beta-glucoside (pNP-Glc) can be used to assay beta-glucosidase activity. 4-Nitrophenyl beta-globotrioside (pNP-Glo) is a similar compound that has been designed as a substrate for glycosidases that cleave terminal beta-galactosyl-(1->3)-N-acetylgalactosamine residues. In these assays, the release of 4-nitrophenol can be measured to determine the activity of the enzyme under study. The use of chromogenic substrates like pNP-Glo allows for the rapid and reliable detection of glycosidase activity in a variety of biological samples.

4-Nitrophenyl alpha-D-maltotetraoside is a chromogenic substrate used for the detection of alpha-glucosidase and alpha-amylase activity. The four glucose units in the molecule are hydrolyzed by the enzyme, releasing 4-nitrophenol and allowing for easy colorimetric measurement of enzymatic activity

4-Nitrophenyl-myo-inositol-1-yl phosphate is a highly specific chromogenic substrate used for the detection, quantification, and characterization of phosphoinositide phosphatases. When cleaved by the target enzyme, it generates a highly absorbing 4-nitrophenyl moiety, producing a yellow color that can be measured spectrophotometrically. This versatile substrate is valuable in examining phosphoinositide phosphatase activity, enzyme kinetics, substrate specificity, and inhibition studies in various cellular processes, including signal transduction, lipid metabolism, and membrane trafficking.