Substrats d'enzymes

<p>2-Chloro-4-nitrophenyl b-D-maltoside is a fluorescent substrate used in diagnostic kits. It is used to detect the presence of beta-glucuronidase enzymes, which are found in many bacteria and fungi. 2-Chloro-4-nitrophenyl b-D-maltoside has been shown to be a superior substrate for beta-glucuronidase as it can be used at lower concentrations and is more stable than other substrates. The enzyme hydrolyzes the 2,4 nitrophenyl ester bond and releases the fluorogenic product 4-(2'-hydroxyethyl)benzene diazonium chloride (HED). This product emits light under ultraviolet light, making it ideal for use in Bioluminescence Assays.</p>

<p>Water soluble substrate for visualizing alkaline phosphatase activity. Produces a magenta colored insoluble end product that is detected visually, when used together with nitroblue tetrazolium. The substrate system is versatile and functions in a variety of applications, including Northern, Southern, and Western blotting, in situ hybridization, ELISAs and immunohistochemistry.</p>

<p>5-Bromo-4-Chloro-3-Indoxyl-N-Acetyl-Beta-D-Galactosaminide is a chromogenic substrate for detecting beta-galactosidase and GalNAc-transferase enzymes. Upon hydrolysis by the enzyme, it produces a blue-green colored compound that can be detected visually or measured spectrophotometrically. This substrate is useful in characterizing the activity of Beta-Galactosidase and GalNAc-transferase enzymes involved in glycobiology research and diagnostic applications.</p>

<p>Chromogenic substrate for beta-glucuronidase (GUS) yielding blue precipitate upon cleavage. Used for identification of beta-glucuronidase. X-beta-D-glucuronide has been reported as chromogenic substrate in a number of chromogenic E. coli/ coliform culture media. About 95% of common E. coli possess beta-D-glucuronidase activity. Such chromogenic culture media are used for detection and enumeration of E. coli in food samples such as meat, dairy products and shellfish and have clinical applications in the assessment of urinary infections. It has gained international acceptance as highly accurate test for the presence of E. coli in drinking water samples. It is also used for the detection of the expression of GUS (E. coli beta-glucuronidase) gene fusion marker constructs in plants. The sodium salt has advantages over the cyclohexylammonium salt as being non-electrostatic and possessing a naturally-occurring cation.</p>

<p>Please enquire for more information about L-Phenylalanine 7-amido-4-methylcoumarin, trifluoroacetate salt including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>4-Nitrophenyl-beta-L-fucopyranoside is a chromogenic substrate used in the determination of alpha-glucosidase activity. The formation of phenol from 4-nitrophenyl leads to the production of a yellow color that can be quantified by measuring its absorbance at 490 nm. This assay is based on the principle that alpha-glucosidase hydrolyzes sucrose into glucose and fructose, which are then oxidized to form 4-nitrophenol and 4-nitroanomeric acid, respectively. The reaction product is detected by measuring the absorption at 490 nm.</p>

<p>4-Methylumbelliferyl 3-deoxy-D-glycero-a-D-galacto-2-nonulosonic acid sodium salt is a fluorogenic substrate that can be used to detect enzyme activity. It reacts with the enzyme β-galactosidase, which hydrolyzes lactose in the presence of oxygen and hydrogen peroxide. The product is 4methylumbelliferone, which emits light in the presence of luciferin and an oxidizing agent. This product is used for diagnostics, food testing, and environmental testing. CAS No. 123269-95-4</p>

<p>Chromogenic substrates targeting beta-xylosidase</p>

<p>4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt is a high-quality fluorogenic substrate specifically designed for the detection and quantification of various enzymes, including beta-glucosidases and sulfatases. Upon hydrolysis by the target enzyme, this substrate releases the highly fluorescent 4-methylumbelliferone, allowing for real-time monitoring and accurate measurement of enzyme activities in biochemical assays, diagnostic tests, and research applications. 4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt has excellent solubility in water, ensuring a consistent and reliable performance in different experimental conditions. This versatile substrate is an essential tool for researchers and industry professionals who require a sensitive and reproducible detection method for enzymes of interest.</p>

<p>N-CBZ-L-Lysyl-L-lysyl-L-arginine 7-amido-4-methylcoumarin triacetate is a synthetic peptide substrate used as a fluorogenic substrate for aminopeptidase.</p>

<p>Fluorogenic substrate for beta-glucosidase yielding a blue fluorescent solution. Used for the detection of beta-glucosidase as an indicator of Enterococci.</p>

<p>4-Methylumbelliferyl-beta-L-fucopyranoside is a fluorogenic substrate that is used in food testing. It also has uses as a chromogenic substrate and a ligand for biological studies. 4-Methylumbelliferyl-beta-L-fucopyranoside is a conjugate of 4-methylumbelliferone and beta-D-fucopyranosyl ester, which is an enzyme substrate for the detection of phosphatases. This product can be used to detect the activity of phosphatase enzymes in various media, including culture media, environmental testing, and staining. 4MUFBPY also has chemiluminescent properties that can be used for bioluminescence assays.</p>

<p>Chromogenic β-glucosidase substrate yielding a yellow solution upon cleavage.</p>

<p>Chromogenic substrate to visualize beta-glucuronidase activity; produces a red color</p>

<p>4-Nitrophenyl 2-acetamido-2-deoxy-a-D-glucopyranoside is a chromogenic pNP substrate specifically designed for the analysis of N-acetylglucosaminidase activity. Upon enzyme action, the substrate releases 4-nitrophenol, a yellow compound that can be detected by spectrophotometric methods, providing a reliable and sensitive means of quantifying enzyme activity. This versatile substrate is widely used in biochemical studies, disease diagnostics, and the enzyme production industry.</p>

<p>Substrate for determining hydrolytic activity of chitinase</p>

<p>L-Proline 7-amido-4-methylcoumarin hydrobromide is a fluorogenic substrate that is used to measure esterase and peptidase activity in a variety of animal and microbial sources. This compound has been shown to have a lipase activity. L-Proline 7-amido-4-methylcoumarin hydrobromide is also used as an enzyme preparation and as an enzyme source.</p>

<p>4-Nitrophenyl a-D-thiomannopyranoside is a chemiluminescence substrate that can be used to measure the activity of β-galactosidase, an enzyme involved in the hydrolysis of lactose. This product is available as a white to yellowish powder and has been shown to have high purity and quality. It also displays strong fluorescence when reacted with hydrogen peroxide and has been used for diagnostics tests such as staining, food testing, and enzyme substrate reactions.</p>

<p>Luciferin-alpha-D-glucoside, potassium salt is a bioluminescence substrate for the detection of various enzymes. It is used to detect the presence of alkaline phosphatase, acid phosphatase, and diaphorase. Luciferin-alpha-D-glucoside, potassium salt is also used as a chromogenic substrate for the determination of glucose in blood and urine, as well as a fluorogenic substrate for the determination of uric acid in urine. This product has high purity and quality and is available in conjugates with antibodies or other ligands that are useful for immunoassays.</p>

<p>4-Chloro-3-indoxyl-1,3-diacetate is a fluorogenic or chromogenic substrate used in food testing. It is also an enzyme substrate that can be used to detect the presence of bioluminescence. 4-Chloro-3-indoxyl-1,3-diacetate has been shown to have high purity and high quality for use in diagnostic kits. 4-Chloro-3-indoxyl-1,3-diacetate is a ligand that is conjugated with other molecules to form complexes for use in fluorescence and chemiluminescence experiments.</p>