Substrats d'enzymes

<p>AquaSpark® β-D-glucoside is a dioxetane-based water-soluble substrate for β-glucosidase activity. In microbiology, β-glucosidase probes can be used for the detection of Listeria spp., Enterococcus spp.,Klebsiella pneumoniae and other species.</p>

<p>5-Bromo-6-chloro-3-indolyl a-D-galactopyranoside is a chromogenic substrate that yields a magenta-colored product after cleavage. The chromophore 5-bromo-6-chloro-indoxyl is released, generating the observable color. It is used in glycolipid metabolism studies.</p>

<p>Resorufin b-D-xylobioside is a fluorogenic substrate for xylanases useful for xylanase activity assays.</p>

<p>Modified naphtol AS substrate used in histochemical and cytochemical staining</p>

<p>L-Histidine 7-amido-4-methylcoumarin hydrochloride is a synthetic peptide substrate used as a fluorogenic substrate for histidine aminopeptidase.</p>

<p>4-Nitrophenyl phosphate mono(2-amino-2-ethyl-1,3-propanediol) salt monohydrate is a chromogenic substrate that can be used to detect the presence of alkaline phosphatase. It is also used in food testing and diagnostics. 4NPPMD is a substrate for the enzyme alkaline phosphatase, which has been shown to play a role in the inflammatory response. The production of 4NPPMD by bacteria in the environment can be detected with chemiluminescence or bioluminescence. This product has been shown to react with both horseradish peroxidase and alkaline phosphatase, as well as other enzymes that are involved in metabolic pathways. This substrate also has high purity and fluorescence properties.</p>

<p>Coelenterazine and its derivatives spontaneously complex with aequorin to develop a Ca2+-dependent fluorescent signal.</p>

<p>A fluorogenic substrate for detection of enzymatic activity of the SARS-CoV-2 Mpro protease. The fluorophore 7-Amino-4-methylcoumarin (AMC) is linked to the tetrapeptide via a peptide bond at the C-terminal of the glutamine residue. Following the hydrolytic activity of the Mpro protease, the fluorogenic AMC group get released from the substrate, generating a fluorescent signal (Ex 380 nm / Em 455 nm).</p>

<p>5-Bromo-4-chloro-3-indoxyl-beta-D-glucuronic acid, sodium salt anhydrous is a potent inhibitor of histidine decarboxylase (HDC). It is used in the histochemical staining of dehydrogenase and effector proteins in urine samples. This compound has been shown to inhibit the growth of macroalgae and can be used as a selective agent for phylogenetic tree construction. 5BDG,Sr with its high specificity for HDC, also inhibits cell proliferation in plant cells and ovules.</p>

<p>Aldol® 458 phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatase activity. The colorless enzyme substrate is cleaved by phosphatases secreted by bacteria grown in liquid media, yielding yellow color and green fluorescence. Aldol® 458 phosphate can also be used in assays involving phosphatase-coupled antibodies in immunoassay applications (ELISA). Aldol® 458 phosphate can be used under aerobic and anaerobic conditions.</p>

<p>Aldol® 455 alpha-D-glucopyranoside is a synthetic fluorescent sugar that has been used in the diagnosis of bacterial infections. It has been used as a substrate for the enzyme beta-galactosidase and as a conjugate with anti-bacterial antibodies, which can be used to detect bacteria in urine and blood samples. Aldol® 455 alpha-D-glucopyranoside is also commonly used in the detection of bacteria in food and environmental samples. The product has a high purity level and quality.</p>

<p>Chromogenic substrate for β-D-fucosidase yielding a blue precipitate.</p>

<p>STAADIUM(TM) PeptiZide L-Ala, Patent pending is a chemiluminescent substrate that reacts with hydrogen peroxide to produce light. It can be used in diagnostic applications such as the detection of blood or fecal stains and in tests for the presence of enzymes or antibodies. STAADIUM(TM) PeptiZide L-Ala, Patent pending is an excellent choice for use with bacterial culture media and food testing. This product has been shown to have high purity and quality.</p>

<p>1-Naphthyl beta-D-glucuronide (NPG) is a chromogenic enzyme substrate used to detect the presence of β-glucuronidase activity. Upon hydrolysis by the enzyme, it produces a yellow product that can be detected by spectrophotometry or visually. It is commonly used in clinical assays to detect the presence of the enzyme in urine samples, as elevated levels can indicate liver or urinary tract diseases. It is also utilized in environmental testing, as some bacteria strains can produce the enzyme as a by-product, making NPG a useful tool for detecting these strains in contaminated water or food sources.</p>

<p>Also known as Lapis-caprylate; targeting esterase with C8 activity; Blue color</p>

<p>Chromogenic substrate for beta-glucanase</p>

<p>4-Methylumbelliferyl-alpha-L-rhamnopyranoside is a fluorogenic substrate used in enzyme assays. It is a substrate of the enzyme beta-galactosidase, which catalyzes the hydrolysis of terminal galactose residues from polysaccharides and glycoproteins. The fluorescent product 4-methylumbelliferone (4MU) is formed by cleavage of the terminal galactose residue and can be measured spectrophotometrically at 360 nm. 4-Methylumbelliferone has been used for chemiluminescent detection in immunoassays and as a substrate for bioluminescence reactions.</p>

<p>Ac-DEVD-6-amino-D-luciferin is a substrate for luciferase that can be used in diagnostic, bioluminescence, and chemiluminescence assays. Ac-DEVD-6-amino-D-luciferin is a high quality compound that has been conjugated with various ligands. The CAS number for Ac-DEVD-6-amino-D-luciferin is 612061–87–8.</p>

<p>4-Methylumbelliferyl beta-D-cellohexaoside is a fluorogenic substrate for cellulase. After enzymatic cleaveage, free 4-methylumbelliferone (also known as hymecromone) is released, exhibiting blue fluorescence upon excitation with UV light. The strongest fluorescence of 4-methylumbelliferone requires deprotonation of the hydroxyl group (thus requires alkaline pH), with a maximal fluorescence intensity obtained with excitation at 350 to 370 nm and emission at 440 to 470 nm. The use of 4-methylumbelliferyl beta-D-cellohexaosideas a substrate for measuring the cellulase activity is used for detecting bacterial infection and gut microbiome analysis.</p>

<p>2'-(4-Methylumbelliferyl)-a-D-N-glycolylneuraminic acid is a fluorogenic substrate that is used for enzymatic labeling of glycolipids. It can be used as a diagnostic or food testing agent, and also has applications in environmental testing. The CAS number for this product is 1434265-32-3.</p>