Substrats d'enzymes

<p>5-Bromo-4-Chloro-3-Indoxyl-N-Acetyl-Beta-D-Galactosaminide is a chromogenic substrate for detecting beta-galactosidase and GalNAc-transferase enzymes. Upon hydrolysis by the enzyme, it produces a blue-green colored compound that can be detected visually or measured spectrophotometrically. This substrate is useful in characterizing the activity of Beta-Galactosidase and GalNAc-transferase enzymes involved in glycobiology research and diagnostic applications.</p>

<p>Water soluble substrate for visualizing alkaline phosphatase activity. Produces a magenta colored insoluble end product that is detected visually, when used together with nitroblue tetrazolium. The substrate system is versatile and functions in a variety of applications, including Northern, Southern, and Western blotting, in situ hybridization, ELISAs and immunohistochemistry.</p>

<p>4-Methylumbelliferyl 3-deoxy-D-glycero-a-D-galacto-2-nonulosonic acid sodium salt is a fluorogenic substrate that can be used to detect enzyme activity. It reacts with the enzyme β-galactosidase, which hydrolyzes lactose in the presence of oxygen and hydrogen peroxide. The product is 4methylumbelliferone, which emits light in the presence of luciferin and an oxidizing agent. This product is used for diagnostics, food testing, and environmental testing. CAS No. 123269-95-4</p>

<p>Chromogenic substrate for beta-glucuronidase (GUS) yielding blue precipitate upon cleavage. Used for identification of beta-glucuronidase. X-beta-D-glucuronide has been reported as chromogenic substrate in a number of chromogenic E. coli/ coliform culture media. About 95% of common E. coli possess beta-D-glucuronidase activity. Such chromogenic culture media are used for detection and enumeration of E. coli in food samples such as meat, dairy products and shellfish and have clinical applications in the assessment of urinary infections. It has gained international acceptance as highly accurate test for the presence of E. coli in drinking water samples. It is also used for the detection of the expression of GUS (E. coli beta-glucuronidase) gene fusion marker constructs in plants. The sodium salt has advantages over the cyclohexylammonium salt as being non-electrostatic and possessing a naturally-occurring cation.</p>

<p>Please enquire for more information about L-Phenylalanine 7-amido-4-methylcoumarin, trifluoroacetate salt including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>4-Nitrophenyl-beta-L-fucopyranoside is a chromogenic substrate used in the determination of alpha-glucosidase activity. The formation of phenol from 4-nitrophenyl leads to the production of a yellow color that can be quantified by measuring its absorbance at 490 nm. This assay is based on the principle that alpha-glucosidase hydrolyzes sucrose into glucose and fructose, which are then oxidized to form 4-nitrophenol and 4-nitroanomeric acid, respectively. The reaction product is detected by measuring the absorption at 490 nm.</p>

<p>4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt is a high-quality fluorogenic substrate specifically designed for the detection and quantification of various enzymes, including beta-glucosidases and sulfatases. Upon hydrolysis by the target enzyme, this substrate releases the highly fluorescent 4-methylumbelliferone, allowing for real-time monitoring and accurate measurement of enzyme activities in biochemical assays, diagnostic tests, and research applications. 4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt has excellent solubility in water, ensuring a consistent and reliable performance in different experimental conditions. This versatile substrate is an essential tool for researchers and industry professionals who require a sensitive and reproducible detection method for enzymes of interest.</p>

<p>Fluorogenic substrate for beta-glucosidase yielding a blue fluorescent solution. Used for the detection of beta-glucosidase as an indicator of Enterococci.</p>

<p>4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt is a high-quality fluorogenic substrate specifically designed for the detection and quantification of various enzymes, including beta-glucosidases and sulfatases. Upon hydrolysis by the target enzyme, this substrate releases the highly fluorescent 4-methylumbelliferone, allowing for real-time monitoring and accurate measurement of enzyme activities in biochemical assays, diagnostic tests, and research applications. 4-Methylumbelliferyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate sodium salt has excellent solubility in water, ensuring a consistent and reliable performance in different experimental conditions. This versatile substrate is an essential tool for researchers and industry professionals who require a sensitive and reproducible detection method for enzymes of interest.</p>

<p>N-CBZ-L-Lysyl-L-lysyl-L-arginine 7-amido-4-methylcoumarin triacetate is a synthetic peptide substrate used as a fluorogenic substrate for aminopeptidase.</p>

<p>Naphthol AS-BI sulphate potassium salt is a modified naphtol AS substrate used for alkaline and acid phosphatase.</p>

<p>3-Indoxyl sulfate, potassium salt (3ISK) is a chemical compound that is soluble in water. It has been used as a marker for bowel disease, and it has been shown to react with analytical methods such as titration calorimetry and lc-ms/ms. 3ISK can be used to determine the optimum concentration for pharmacological treatment of bowel disease. The metabolic fate of 3ISK has been studied extensively and it is found that 3ISK is metabolized by microbial enzymes in the gut, which may lead to an increased incidence of bowel disease in patients with low levels of microbial metabolism. 3ISK also plays an important physiological function by maintaining mitochondrial membrane potential, which could have implications for pharmacological treatment of bowel disease.</p>

<p>Chromogenic β-glucosidase substrate yielding a yellow solution upon cleavage.</p>

<p>Chromogenic substrate to visualize beta-glucuronidase activity; produces a red color</p>

<p>4-Methylumbelliferyl-beta-L-fucopyranoside is a fluorogenic substrate that is used in food testing. It also has uses as a chromogenic substrate and a ligand for biological studies. 4-Methylumbelliferyl-beta-L-fucopyranoside is a conjugate of 4-methylumbelliferone and beta-D-fucopyranosyl ester, which is an enzyme substrate for the detection of phosphatases. This product can be used to detect the activity of phosphatase enzymes in various media, including culture media, environmental testing, and staining. 4MUFBPY also has chemiluminescent properties that can be used for bioluminescence assays.</p>

<p>4-Chloro-3-indoxyl-1,3-diacetate is a fluorogenic or chromogenic substrate used in food testing. It is also an enzyme substrate that can be used to detect the presence of bioluminescence. 4-Chloro-3-indoxyl-1,3-diacetate has been shown to have high purity and high quality for use in diagnostic kits. 4-Chloro-3-indoxyl-1,3-diacetate is a ligand that is conjugated with other molecules to form complexes for use in fluorescence and chemiluminescence experiments.</p>

<p>L-Proline 7-amido-4-methylcoumarin hydrobromide is a fluorogenic substrate that is used to measure esterase and peptidase activity in a variety of animal and microbial sources. This compound has been shown to have a lipase activity. L-Proline 7-amido-4-methylcoumarin hydrobromide is also used as an enzyme preparation and as an enzyme source.</p>

<p>4-Nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate potassium is a chromogenic substrate for N-acetylglucosamine-6-sulfatase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 2-acetamido-2-deoxy-beta-D-glucopyranoside-6-sulfate potassium is used in different applications such as the Mucopolysaccharidosis IIID (Sanfilippo D) screening</p>

<p>A sensitive fluorogenic substrate for fluorometric and UV-spectrophotometric assays of phosphatases. It is used for the detection of C. perfringens because acid phosphatase is a highly specific indicator for C. perfringens.</p>

<p>2-Nitrophenyl a-D-glucopyranoside is a chromogenic substrate commonly used to assay the activity of glucosidases. Upon cleavage of the glycosidic bond by glucosidases, the released 2-nitrophenyl group yields a yellow color detectable by spectrophotometry. This substrate has high sensitivity and is widely used in a variety of applications, including drug discovery and enzyme kinetics studies.</p>