Substrats chromogènes

Chromogenic β-glucuronidase substrate yielding a yellow solution upon cleavage. Used to detect E. coli contamination in food and drinking water due to their β-glucuronidase activity.

Chromogenic sulfatase substrate

5-Bromo-4-Chloro-3-Indoxyl-N-Acetyl-Beta-D-Galactosaminide is a chromogenic substrate for detecting beta-galactosidase and GalNAc-transferase enzymes. Upon hydrolysis by the enzyme, it produces a blue-green colored compound that can be detected visually or measured spectrophotometrically. This substrate is useful in characterizing the activity of Beta-Galactosidase and GalNAc-transferase enzymes involved in glycobiology research and diagnostic applications.

For detection of beta-galactosidase activity.  The colors of the chromogen can be adjusted by incorporating different functional groups, e.g. cyano or nitro group, which yields purple or black green colonies with beta-galactosidase positive strain.

Aldol® 484 beta-D-galactopyranoside is a chromogenic enzyme substrate for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid or solid media, yielding intense red color. Aldol® 484 beta-D-galacto-pyranoside can also be used in beta-gal reporter gene assays or for identification of beta-gal positive transformants on agar. Aldol® 484 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.

6-Bromo-2-naphthyl β-D-galactopyranoside is a chromogenic substrate commonly used for the detection of the enzymatic activity of β-galactosidase. It can produce a yellow precipitate upon hydrolysis by β-galactosidase, indicating the presence of the enzyme. It is often used in molecular biology applications to detect gene expression or to monitor cloning efficiency.

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Chlorophenol red-b-D-galactopyranoside sodium salt is the salt from of CPRG (chlorophenol red-b-D-galactopyranoside), that has a higher solubility. It is a chromogenic substrate for beta-galactosidases that, after enzymatic cleavage, releases chlorophenol red, a dark red compound quantified by absorbance at 570 nm.

5-Bromo-4-chloro-3-indolyl a-D-maltopyranoside is a chromogenic substrate used to detect the specific enzymatic activity of alpha-glucosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl a-D-maltopyranoside is used in Pompe disease screening and glycogen storage disorder diagnosis.

4-Nitrophenyl 3-O-(a-D-glucopyranosyl)-a-D-glucopyranoside (pNP-Glc-Glc) is a highly sensitive and potent chromogenic substrate for the enzymatic detection and measurement of alpha-glucosidase activity. The rapid color change triggered by the hydrolysis of pNP-Glc-Glc provides a clear and efficient method for monitoring enzyme kinetics, screening inhibitors, and functional characterization of α-glucosidases. This substrate is soluble in aqueous and polar organic solvents and yields a detectable 4-nitrophenol product with a distinct yellow color under both visible and ultraviolet light, enabling quantitative spectrophotometric measurements. With a superior signal-to-noise ratio and maximal sensitivity, pNP-Glc-Glc is a reliable and robust option for biochemical and biomedical research involving glucosidase enzymes.

Chromogenic substrate for α-L-Fucosidase. 2-naphthol is released upon hydrolyzation. By simultaneous coupling with a suitable staining reagent, such as hexazonium p-rosaniline, the corresponding azo-dye is formed. Naphthols can also be detected by fluorescence analysis.

5-Bromo-4-chloro-3-indolyl a-L-arabinofuranoside is a chromogenic substrate used to detect the specific enzymatic activity of alpha-L-arabinofuranosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl a-L-arabinofuranoside is used in bacterial arabinan degradation assays (e.g. Bacillus spp.).

Chromogenic substrate yielding a soluble yellow chromophor for the colorimetric assay of phosphatases.

2-Naphthyl caprylate is a substrate that is employed in an assays for carboxylesterase and lipase and when hydrolyzed, produces 2-naphthol, a compound that can also be identified via fluorescence analysis. By combining it with a diazonium salt, the formation of the corresponding azo dye occurs. In particular, this substrate is used to detect the extracellular lipase of Pseudomonas fluorescens B52.

2-Fluoro-4-nitrophenyl b-D-galactopyranoside is a chromogenic substrate in enzymatic assays with β-galactosidase. Commonly abbreviated as PFONPG, it is a hydrophilic compound that can be dissolved in saline and blood samples, which makes it a suitable candidate for diagnostic applications as a gene reporter probe by simple analytic methodologies. 2-Fluoro-4-nitrophenyl b-D-galactopyranoside have been used to detect and monitor the growth of prostate tumor cells.

6-Chloro-3-indoxyl-beta-D-glucuronic acid methyl ester is a chromogenic substrate that can be used for the detection of indoxyl glucuronide in urine and other biological fluids. 6-Chloro-3-indoxyl-beta-D-glucuronic acid methyl ester is a white or light yellow crystalline solid that is soluble in water. It has a melting point of about 80°C and is stable at pH levels ranging from 2 to 12. The CAS number for this compound is 13982-87-6.