Substrats chromogènes

<p>2-Nitrophenyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-a-D-glucopyranoside is a chromogenic substrate commonly used in enzyme assays for the detection and quantification of enzyme activity. It is a colorless substrate that is enzymatically converted into a yellowish product, the intensity of which is directly proportional to the enzyme activity present in the sample.</p>

<p>4-Nitrophenyl 2-acetamido-6-O-(2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-glucopyranosyl)-3-O-(2,3,4,6-tetra -O acetyl -b D galactopyranosyl)-2 deoxy a D galactopyranoside is a high purity fluorogenic substrate for the enzyme β galactosidase. It can be used for the detection of β galactosidase in biological samples. 4NPAG can also be used as a ligand for various types of chromogenic substrates. This product is also commonly used in diagnostics and conjugates to detect β galactosidase activity or as a culture media supplement.</p>

<p>S-(4-Nitrophenyl)mercapturic acid (S-NPMA) is the major metabolite of 4-nitrophenol, which is a precursor to dyes and pharmaceuticals. S-NPMA has been detected in urine samples from humans, rats, and mice. The main metabolic pathway for S-NPMA is an oxidative conversion by cytochrome P450 enzymes. S-NPMA has been found to be a useful biomarker for the presence of polycyclic aromatic hydrocarbons in wastewater and human urine. The levels of this compound have also been shown to increase with age in humans.</p>

<p>N-Acetyl-L-serine p-nitroanilide is a fluorescent probe used for the detection of glucose in urine. It has a high purity and is suitable for use as a substrate for many enzymes, including alkaline phosphatase, β-galactosidase, and peroxidase. N-Acetyl-L-serine p-nitroanilide has been shown to be an excellent indicator of bacterial growth in culture media.</p>

<p>4-Nitrophenyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-a-D-glucopyranoside is a synthetic compound with the ability to inhibit the enzyme glutamicum. It is a radionuclide that can be used as a focusing agent for gamma cameras and has been shown to be effective against corynebacterium glutamicum and other organisms. 4NP2AG was synthesized by reacting nitrophenol with acetamide in the presence of an acid catalyst and hydrogenating the product. The synthesis employs precession chemistry, which involves rotating a molecule around its axis at high speed to increase its concentration in one area. This treatment leads to a higher yield of desired product. 4NP2AG contains two acetamido groups on the glucopyranoside moiety, which are esterified with nitrophenol and acetic acid respectively. These moieties are</p>

<p>2-Nitrophenyl 6-O-trityl-b-D-galactopyranoside is a chromogenic enzyme substrate used in assays to detect galactosidase activity. It is a derivative of galactose that can be hydrolyzed by galactosidase to release the yellow-colored 2-nitrophenyl group, which can be detected spectrophotometrically. The trityl group helps to stabilize the molecule and prevent undesired hydrolysis during storage. This substrate is commonly used in the study of carbohydrate metabolism and in the detection of galactosidase activity in microorganisms.</p>

<p>Aldol® 515 palmitate is a high quality, non-toxic and water soluble chromogenic substrate for enzymes. It is used to detect the presence of lipase activity due to yielding a red color.</p>

<p>4-Nitrophenyl 2-O-(α-L-fucopyranosyl)-β-D-galactopyranoside is a chromogenic enzymatic substrate for the detection and quantification of glycosidases, such as fucosidases and galactosidases. Upon enzymatic cleavage, the substrate releases the p-nitrophenol chromophore, which can be quantified via colorimetric analysis. This pNP-conjugated compound enables the rapid, sensitive, and high-throughput detection of enzyme activity in various applications, including enzymatic assays, inhibitor screening, and mechanistic studies. Its precise specificity for α-L-fucopyranosyl and β-D-galactopyranoside moieties makes it an excellent tool for advancing research in glycobiology and related fields.</p>

<p>4-Nitrophenyl O-(N-acetyl-a-neuraminosyl)-(2-3)-b-D-galactopyranosyl-(1-4)-b-D-glucopyranoside is a fluorescent substrate that can be used for the detection of β-galactosidase. It fluoresces when cleaved by β-galactosidase, which is an enzyme that catalyzes the hydrolysis of terminal galactose residues in the glycosidic linkage of β-galactosides. This product is an excellent substrate for use in chemiluminescence and bioluminescence assays. 4NPBG has been used to detect β-galactosidase activity in diverse environments, such as food testing, staining, fluorogenic substrate, environmental testing, chromogenic substrate, and conjugate. It is also used in diagnostics, ligand binding assays,</p>

<p>N-Acetyl-2-nitrophenyl 6-O-DMT-b-D-galactopyranoside is a fluorogenic substrate that is used in enzyme and chromogenic assays, including the determination of aminoglycoside activity. This product has been shown to be suitable for detection of bacterial contamination in food products and environmental samples. N-Acetyl-2-nitrophenyl 6-O-DMT-b-D-galactopyranoside reacts with aminoglycosides at the O4 position, forming a fluorescent product that can be measured using a fluorometer.</p>

<p>N-a-Boc-N-epsilon-cbz-L-lysine 4-nitroanilide is a compound that has been shown to regulate the production of angiotensin. It also acts as an inhibitor for the production of fibronectin, which is key in the regulation of inflammation and wound healing. N-a-Boc-N-epsilon-cbz-L-lysine 4 nitroanilide has been shown to be a trifunctional molecule and can yield three different products: oxychlorides, hydrated and solvates. This molecule also regulates phosphorus levels. There are many functions that this molecule performs, including binding with amino acids and regulating their function, which is why it is so important to study this molecule more closely.</p>

<p>2-O-(2-Nitrophenyl)-a-D-N-acetylneuraminic acid is a chromogenic substrate specifically designed for the detection and quantification of sialidase (neuraminidase) enzyme activity. Neuraminidases are enzymes that cleave sialic acid residues from glycoconjugates, and are produced by many microorganisms, including influenza viruses. Upon cleavage by sialidase, this substrate releases a yellow-colored 2-nitrophenol product, which can be easily monitored spectrophotometrically. This substrate is widely used in various applications, including enzyme kinetics studies, inhibitor screening, and the detection of bacterial and viral neuraminidases in clinical and environmental samples.</p>

<p>4-Nitrophenyl O-(N-acetyl-a-neuraminosyl)-(2-3)-b-D-galactopyranosyl-(1-4)-2-acetamido-2-deoxybDglucopyranoside is a fluorescent compound that has been used as a substrate in enzyme assays. It has been shown to be an effective ligand for the enzyme beta galactosidase, which hydrolyzes the substrate to produce 4nitrophenol and o-(Nacetyl)a-neuraminic acid. This product is CAS No. 501427-92-5 and can be used as a high purity, high quality reagent for conjugation.</p>

<p>4-Nitrophenyl 2-acetamido-2-deoxy-3-O-(a-D-galactopyranosyl)-a-D-galactopyranoside is a fluorescent substrate that can be used in chemiluminescent assays. It is a high purity, high quality chemical that has been tested for culture media, environmental testing and bioluminescence. This product is an excellent choice for use in diagnostics, chromogenic substrates, enzyme substrates and food testing.</p>

<p>4-Nitrophenyl 4,6-cyclohexylidene-b-D-mannopyranoside is a fluorogenic substrate that can be used for the detection of β-glucosidase activity. It is a high purity product with a ligand that binds specifically to β-glucosidase. 4NPPM is an enzyme substrate that can be used in diagnostics for the detection of β-glucosidase activity and conjugate for chemiluminescence and bioluminescence. This product has been shown to react with glucose in the presence of hydrogen peroxide and luminol, emitting light when excited by ultraviolet light at 365 nm. 4NPPM also reacts with glucose oxidase to produce hydrogen peroxide, which then reacts with luminol, producing light. The CAS number for this compound is 102717-16-8.</p>

<p>1-Naphthyl 2-acetamido-3,4,6-tri-O-acetyl-2-deoxy-b-D-glucopyranoside is a fluorogenic substrate for bacterial beta D glucosidase. It is used for measuring the enzyme activity in different types of culture media and in the detection of bacterial contamination in food and environmental samples. This product can be used to detect the presence of beta D glucosidase by measuring luminescence with a luminometer. 1NAAG can also be used as a chromogenic substrate or to conjugate with antibodies for use in immunoassays.br&gt;</p>

<p>4-Nitrophenyl 2-acetamido-3-O-acetyl-2-deoxy-b-D-glucopyranoside is a fluorogenic substrate for the enzyme acetylcholinesterase. It can be used in food testing, environmental testing and diagnostics. 4NP2A2DGBG is a high purity, high quality product with a CAS number of 23262-57-9.</p>

<p>5-Bromo-4-chloro-3-indolyl 2-acetamido-2-deoxy-a-D-glucopyranoside is a fluorogenic substrate that reacts with horseradish peroxidase to produce light. It is used in the detection of β-galactosidase activity in food testing, staining, and environmental testing. This product is also used as a chromogenic substrate for the determination of β-galactosidase activity in immunological assays. 5BICAPG is a conjugate that can be used as a diagnostic ligand for the detection of β -galactosidase enzyme activity. This product has been shown to react with horseradish peroxidase and produce chemiluminescence or bioluminescence when exposed to oxygen. 5BICAPG has also been found to be an excellent enzyme substrate for various enzymes including alkaline phosphatase</p>

<p>5-Bromo-6-chloro-3-indoxyl nonanoate is a chemical compound that is used in the detection of bioluminescence. It is a substrate for enzymes, such as luciferase, and can be used to measure the amount of light emitted during the reaction. The CAS number for this molecule is 682802-80-8.</p>

<p>Neu5Ac(a2-6)Gal(b1-3)GlcNAc-b-pNP is a fluorogenic substrate that can be used for testing the presence of glycoproteins in food samples. It also has been used to detect the presence of enzymes in culture media. Neu5Ac(a2-6)Gal(b1-3)GlcNAc-b-pNP is a high purity product that has a CAS number and is available as a conjugate. This product can be purchased in bulk quantities, with a purity of &gt;90%.</p>

<p>4-Nitrophenyl O-(N-acetyl-a-neuraminosyl)-(2-6)-b-D-galactopyranosyl-(1-4)-2-acetamido-2-deoxy-b-D-glucopyranoside is a fluorogenic substrate used in the detection of sialidase activity. It is also used as a chromogenic substrate in the determination of β -galactosidase activity.</p>

<p>4-Nitrophenyl (N-acetyl-a-neuraminosyl)-(2,3)-O-b-D-galactopyranosyl-(1,3)-2-acetamido-2-deoxy-b-D-galactopyranoside is a fluorogenic substrate that is used in the detection of enzyme activity. It reacts with a specific enzyme to produce light. This product has been shown to be useful for detecting β -galactosidase, β -glucuronidase, α -mannosidase, and lactose permeases. The product is also used in the diagnosis of urinary tract infections and other diseases. This product is not intended for use in humans or animals.</p>

<p>4-Nitrophenyl 2,3,4-tri-O-levulinoyl-a-D-mannopyranoside is a fluorogenic substrate that contains a reactive group of 2,3,4-tri-O-levulinoyl. This compound can be used to measure the activity of enzymes such as beta-glucosidase and alpha-galactosidase. 4NP2OMP is an excellent ligand for metal ions and has been shown to bind to copper in the presence of ATP. 4NP2OMP can also be used as a diagnostic agent for measuring the activity of bacterial beta-glucosidase and alpha galactosidase.</p>

<p>Butyl 4-nitrophenyl hexyl phosphonate is a lipase suicide inhibitor that is a chromogenic substrate for lipases which upon enzymatic action, binds irreversibly to the lipase and inactivates it. The substrate can be used for the determination of active sites in lipases, especially in lipases preferring long chain leaving groups.</p>

<p>2-Chloro-4-nitrophenyl b-D-cellobioside (CNPG) is a chromogenic enzyme substrate that is commonly used to detect and measure the activity of β-glucosidases. CNPG is cleaved by the enzyme into the chromogenic product, which can be detected spectrophotometrically. β-glucosidases are important enzymes in the breakdown of cellulose, and CNPG is a widely used substrate for the detection and quantification of these enzymes in various research studies.</p>

<p>4-Nitrophenyl 3,6-di-O-(a-D-mannopyranosyl)-b-D-mannopyranoside is a chemiluminescent substrate that is used in the detection of enzymes. It is also used to measure the activity of beta-glucosidase, alpha-galactosidase, and alkaline phosphatase. The product has been tested for environmental sensitivity and has not shown any effects on bacterial growth. 4-Nitrophenyl 3,6-di-O-(a-D-mannopyranosyl)-b-D-mannopyranoside can be used as a high quality stain for histological sections and immunohistochemical studies. This product has been tested for staining properties with different dyes and has shown no interference with the staining process.</p>

<p>4-Nitrophenyl 2-acetamido-3,6-di-O-acetyl-2-deoxy-b-D-glucopyranoside is a versatile chromogenic pNP enzyme substrate designed to offer superior results for detecting enzyme activity in research and diagnostic applications. This substrate enables faster, more accurate, and reliable detection of hydrolase enzymes, making it ideal for various research and testing needs, such as studying enzyme kinetics, screening inhibitors, and profiling enzyme specificity.</p>

<p>Aldol® 470 L-pyroglutamic acid amide is a chromogenic and fluorogenic enzyme substrate used for detection of pyrrolidonyl peptidase (PYRase) activity. The colorless enzyme substrate is cleaved by PYRase-producing bacteria grown in liquid or solid media, yielding orange color. In addition, green fluorescence is generated when a suitable matrix is present, such as, the surface of cellulose acetate filters, polypropylene tubes or microplates, and polyethylene vials. Aldol® 470 L-pyroglutamic acid amide is suitable for use under aerobic and anaerobic conditions.</p>

<p>4-Nitrophenyl 6-O-benzoyl-3,4-O-isopropylidene-a-D-galactopyranoside (4NBP) is a fluorogenic substrate for β-galactosidase. It has been used as an enzyme substrate in the detection of β-galactosidase activity. 4NBP is also used to detect the presence of galactose in culture media and as a chromogenic substrate in diagnostics.</p>

<p>Tetrabutylphosphonium p-toluenesulfonate is a fluorogenic substrate that is used in the diagnosis of cytochrome P450 enzymes. It is a ligand that binds to metal ions, such as copper and zinc. This compound is also used for the preparation of bioluminescent substrates for use in diagnostics and research.</p>

<p>L-2-Chloro-4-nitrophenyl 2-acetamido-2-deoxy-a-D-glucopyranoside is a chemiluminescent substrate for the detection of aminoglycoside antibiotics. It can be used to determine the concentration of aminoglycosides in blood, urine, and serum. The chemiluminescence reaction is dependent on the presence of oxygen. This product has been shown to have a high quality and purity with environmental testing and staining properties. L-2-Chloro-4-nitrophenyl 2-acetamido-2-deoxy--a--D--glucopyranoside has also been shown to bind ligands such as porphyrins, bilirubin, or hemin with bioluminescence or chromogenic substrates in diagnostics or culture media for food testing. L--2--Chloro--4--</p>

<p>2-Nitrophenyl laurate is a fatty acid that is not able to be degraded and has a high affinity for magnetic particles. It can be used in the immobilization of bacteria, such as Stenotrophomonas maltophilia, which are resistant to many antibiotics. 2-Nitrophenyl laurate also inhibits the enzyme lipolytic enzymes, which are responsible for the degradation of phospholipids in the cell membrane. This inhibition may lead to an increase in bacterial susceptibility to antibiotics. 2-Nitrophenyl laurate is a synthetic substrate that can be used to study the regulation of lipolytic enzymes by regulatory proteins.</p>

<p>Chromogenic substrate targeting amylase and maltase</p>

<p>Introducing our 4-Nitrophenyl 2,3,4-tri-O-acetyl-6-O-(2,3,4,6-tetra-O-[4-methoxybenzyl]-a-D-glucopyranosyl)-a-D-glucopyranoside, an advanced chromogenic pNP enzyme substrate designed for high performance and superior results. This cutting-edge substrate ensures excellent outcomes in various enzymatic assays, including glycosidase and glycosyltransferase, due to its unique composition and significant sensitivity. Offering rapid color development and clear visualization, this substrate is perfect for research or industrial applications.</p>

<p>4-Nitrophenyl 4,6-O-isopropylidene-a-D-galactopyranoside is a fluorogenic substrate that can be used to measure the activity of beta-galactosidase. This product is high purity, high quality and has a CAS number of 29781-31-5. It is an enzyme substrate with chromogenic and diagnostic properties that are used for conjugation to other molecules, as well as for culture media, chemiluminescence and food testing. 4NP4IPG is also used in bioluminescence assays to detect the presence of beta-galactosidase.</p>

<p>2-Nitrophenyl 2-acetamido-3,6-di-O-acetyl-4-O-[2-O-(2,3,4-tri-O-benzoyl-a-L-fucopyranosyl)]--2-deoxy--bDglucopyranoside is an acylated glucopyranoside that is used as a substrate for the enzyme β -galactosidase. When hydrolyzed by β -galactosidase, it yields a blue product that has been widely used in chemiluminescence and bioluminescence assays. It also can be used for staining and ligand binding studies.</p>

<p>4-Nitrophenyl 6-O-DMT-2,3,4-tri-O-levulinoyl-a-D-mannopyranoside is a fluorogenic substrate that can be used for the detection of DMT (dihydroxyacetone) in food products. It has been shown to be an excellent alternative to the more commonly used 4,5′,6,7,8,-Tetrabromoindoxyl 3′,5′,6′,-tetraacetic acid (TBA). This product is also used as a chromogenic substrate for the determination of phenols in environmental testing and as a ligand conjugate for immunohistochemistry staining. The purity of this product is guaranteed and it has been tested extensively.</p>

<p>4-Nitrophenyl 2,3-di-O-(2,3,4,6-tetra-O-acetyl-b-D-glucopyranosyl)-4,6-benzylidene-b-D-glucopyranoside is a high purity, high quality 4nitrophenol derivative that is used as a food testing substrate. It is also used for the detection of fluorescence in environmental testing. This product can be conjugated with ligands for use in diagnostic assays.</p>

<p>5-Bromo-6-chloro-3-indolyl b-D-glucuronide (5BCIG) is an antibiotic that inhibits bacterial growth by binding to the DNA gyrase and topoisomerase IV, which are enzymes that maintain the integrity of bacterial DNA. 5BCIG is detectable in tissues, and quantified in blood. It encodes a protein to the cell cytoplasm. The sensitivity of 5BCIG against clostridium perfringens was found to be 10 times higher than that of penicillin G or erythromycin. 5BCIG has optimal activity against clostridium perfringens when expressed functionally as a polypeptide.</p>

<p>2-Methyl-4-nitrophenyl b-D-galactopyranoside is a chemiluminescent substrate that is used in various applications. It is a ligand that binds to the enzyme beta-galactosidase and is used in diagnostics for detecting the presence of the enzyme. 2-Methyl-4-nitrophenyl b-D-galactopyranoside has been shown to be an appropriate substrate for chemiluminescence reactions, such as those used in diagnostic or research applications. The product also has been tested for potential toxicity and found not to be toxic at concentrations up to 100mg/mL. This product can be used in a variety of applications, including food testing, bacterial culture media, and environmental testing.</p>

<p>4-Nitrophenyl 2,6-di-O-benzoyl-a-D-galactopyranoside is a specialized chromogenic substrate for the accurate evaluation of α-galactosidase activity. Upon enzymatic hydrolysis, it generates the chromophore 4-nitrophenol, which can be conveniently quantified by absorbance spectroscopy. The substrate features a unique benzoylated galactopyranoside moiety for enhanced selectivity and compound stability. This high-quality substrate is perfect for various research, analytical, and diagnostic applications involving α-galactosidase detection or quantification.</p>

<p>4-Nitrophenyl 2-acetamido-3-O-(2,3,4,6-tetra-O-acetyl-b-D-galactopyranosyl)-2-deoxy-a-D-galactopyranoside is a chromogenic substrate designed for the detection and quantification of enzyme activity. This tetra-acetylated compound is specifically designed to be cleaved by enzymes, releasing a yellow 4-nitrophenol product that can be easily measured spectrophotometrically, allowing for accurate and sensitive analysis of enzyme kinetics and inhibition studies.</p>

<p>Espresso-Gal (Espresso-beta-D-galactopyranoside) is a chromogenic indicator for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid media or on agar plates and yields an espresso brown colored precipitate. Espresso-Gal can also be used as detecting agent for beta-galactosidase activity in reporter gene assays or for identification of positive transformants on agar plates when using the α-complementation approach. Read more in the Application Notes.</p>

<p>4-Nitrophenyl 2,3,4,6-tetra-O-acetyl-β-D-glucopyranoside is a versatile chromogenic substrate designed for the detection and quantification of various glycosidase enzymes. This substrate is widely used in enzyme assays, producing a yellow color upon cleavage by target enzymes. The 4-Nitrophenol released in this reaction can be easily monitored spectrophotometrically, allowing for rapid and accurate enzyme analysis. This high-quality substrate provides a convenient and reliable tool for researchers in biochemistry, enzymology, and related fields.</p>

<p>4-Nitrophenyl 2-acetamido-3-azido-2,3-dideoxy-b-D-galactopyranoside is a fluorogenic substrate for beta-galactosidase. It has been used in the detection of beta-galactosidase activity in culture media, and for the detection of beta-galactosidase activity in environmental samples. 4NPAG can be conjugated to a variety of molecules, including antibodies, proteins, nucleotides, and oligonucleotides. 4NPAG can be used as an enzyme substrate for both chromogenic and fluoroquinolone substrates.</p>

<p>4-Nitrophenyl 6-O-trityl-a-D-glucopyranoside is a high-quality trityl pNP substrate that performs excellently in various enzymatic assays. Containing a trityl-protected sugar moiety, this substrate is perfect for investigating alpha-D-glucopyranoside-specific enzymes and providing precise data for your experiments.</p>

<p>Aldol® 455 is a substrate for the enzyme beta-galactosidase. It is used in food testing, staining, and environmental testing. Aldol® 455 has been shown to be a good substrate for fluorogenic and chromogenic substrates. It can be conjugated with an antibody or other ligand for use in immunoassays. Aldol® 455 is of high purity and high quality, making it suitable for use in diagnostics and as an enzyme substrate. This product can also be used as a culture media additive.</p>

<p>4-Nitrophenyl 2-acetamido-3,6-di-O-acetyl-4-O-(2,3,4,6-tetra-O-acetyl-b-D-galactopyranosyl)-2-deoxy-b-D-glucopyranoside is modified acetylated chromogenic substrate designed for the detection and quantification of enzyme activity. This highly specific substrate is cleaved by enzymes, releasing a yellow 4-nitrophenol product that can be easily measured spectrophotometrically. This allows for accurate and sensitive analysis of enzyme kinetics, inhibition studies, and substrate specificity determination.</p>

<p>4-Nitrophenyl 3,4-O-isopropylidene-a-D-galactopyranoside is a chemiluminescent substrate for detecting beta-galactosidase activity in bacteria. This product is a high purity, high quality compound that can be used as a culture media and environmental testing agent. It has been shown to be effective in diagnostics, chromogenic substrate, and enzyme substrate reactions. 4NPPG has been shown to be a fluorogenic substrate for beta-galactosidase assay and can be used as food testing agent with conjugate.</p>

<p>4-Nitrophenyl 4,6-O-benzylidene-3-O-(2,3,4,6-tetra-O-acetyl-b-D-glucopyranosyl)-b-D-glucopyranoside is a high purity fluorescent reagent that can be used as a substrate for many enzymes. It is also used in environmental testing and to detect the presence of nitrophenols in food. This product has CAS number 545414.</p>