Anti-Histone H3.3 (G34V) antibody - 0.5mg/mL

Ref. 3D-CRB2005186

Informations sur le produit

• Nom: Anti-Histone H3.3 (G34V) antibody - 0.5mg/mL

Description

Histone H3.3 is a replication-independent histone variant, which replaces canonical histone H3.1/2 outside of S phase. H3.3 is expressed throughout the cell cycle, as well as in quiescent cells and is referred to as the "replacement histone". H3.3 is largely deposited in a DNA synthesis-independent fashion by a distinct set of chaperones proteins. H3.3 accumulates in post mitotic cells, such as cerebral cortical neurons and is incorporated at sites of UV damage where it protects against sensitivity to UV light. H3.3 deposition occurs on DNA sequences that are transiently nucleosome-free, such as during transcription and DNA repair, therefore serving as a marker for regions of high transcriptional activity. H3.3 is also enriched in heterochromatic subtelomeric and pericentromeric regions. H3.3 plays important roles in many developmental contexts such as in stem cells, during fertilization and reproduction and during reprogramming of genomes following fertilization or somatic cell nuclear transfer. Mutations in histone H3.3 are common events in certain cancers. H3.3 G34V (glycine to valine) substitution mutation is found in paediatric high-grade glioblastoma (HGG).
1mg/ml. For use in WB (1:1000), ELISA (1:1000), IF and IHC. Reacts with Human, Rabbit, Mouse.
Image: Anti-Histone H3 (G34V) antibody (crb2005186) Western blot analysis of cell purified recombinant GST-histone H3 proteins.
Lane 1: GST-histone H3.3 wildtype
Lane 2: GST-histone H3.3 K27M
Lane 3: GST-histone H3.3 G34R
Lane 4: GST-histone H3.3 G34V

• Marque: Biosynth