Rat ASMA(Anti-Smooth Muscle Antibody) ELISA Kit

Ref. EK-ELK0639

Informations sur le produit

• Nom: Rat ASMA(Anti-Smooth Muscle Antibody) ELISA Kit
• Synonymes: ASM Ab; Anti Smooth Muscle Antibody

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat ASMA. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat ASMA. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat ASMA, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat ASMA in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marque: ELK Biotechnology