Cattle E2(Estradiol) ELISA Kit

Ref. EK-ELK0869

Informations sur le produit

• Nom: Cattle E2(Estradiol) ELISA Kit
• Synonymes: 17B-Estradiol; Oestradiol; Beta-Estradiol

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Cattle E2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle E2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle E2 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marque: ELK Biotechnology