Rat CA15-3(Carbonhydrate Antigen 15-3) ELISA Kit

Ref. EK-ELK11235

Informations sur le produit

• Nom: Rat CA15-3(Carbonhydrate Antigen 15-3) ELISA Kit
• Synonymes: CA15-3
• Marque: ELK Biotechnology
• Description: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CA15-3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CA15-3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CA15-3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CA15-3 in the samples is then determined by comparing the OD of the samples to the standard curve.

Propriétés chimiques

• Couleur/Forme: Colourless Transparentliquid