Human BMG/b2-MG(β-2-Microglobulin) ELISA Kit

Ref. EK-ELK1200

Informations sur le produit

• Nom: Human BMG/b2-MG(β-2-Microglobulin) ELISA Kit
• Synonymes: Beta 2M; BMG; B2MG; b2M; Beta-2-Microglobulin

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BMG/b2-MG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BMG/b2-MG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BMG/b2-MG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BMG/b2-MG in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marque: ELK Biotechnology