Rat IP10(Interferon γ Induced Protein 10kDa) ELISA Kit

Ref. EK-ELK2653

Informations sur le produit

• Nom: Rat IP10(Interferon γ Induced Protein 10kDa) ELISA Kit
• Synonymes: CXCL10; C7; IFI10; INP10; SCYB10; Crg2; GIP10; Mob1; Chemokine C-X-C Motif Ligand 10; Small Inducible Cytokine Subfamily B(Cys-X-Cys)Member 10

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat IP10. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat IP10. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat IP10, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat IP10 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marque: ELK Biotechnology