Human MDM2(Mdm2 p53 Binding Protein Homolog) ELISA Kit

Ref. EK-ELK4557

Informations sur le produit

• Nom: Human MDM2(Mdm2 p53 Binding Protein Homolog) ELISA Kit
• Synonymes: HDMX; hdm2; Mouse Double Minute 2,Human Homolog Of p53-Binding Protein; Oncoprotein Mdm2

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human MDM2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human MDM2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human MDM2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human MDM2 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marque: ELK Biotechnology