Human HSD17b1(17-β-Hydroxysteroid Dehydrogenase Type 1) ELISA Kit

Ref. EK-ELK4895

Informations sur le produit

• Nom: Human HSD17b1(17-β-Hydroxysteroid Dehydrogenase Type 1) ELISA Kit
• Synonymes: EDH17B2; EDHB17; HSD17; SDR28C1; Estradiol 17-Beta-Dehydrogenase 1; Short Chain Dehydrogenase/Reductase Family 28CE,1; 20 alpha-hydroxysteroid dehydrogenase

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human HSD17b1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human HSD17b1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human HSD17b1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human HSD17b1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marque: ELK Biotechnology