Human GDF6(Growth Differentiation Factor 6) ELISA Kit

Ref. EK-ELK4974

Informations sur le produit

• Nom: Human GDF6(Growth Differentiation Factor 6) ELISA Kit
• Synonymes: BMP13; CDMP2; GDF16; Bone morphogenetic protein 13; Growth/differentiation factor 16

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GDF6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GDF6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GDF6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GDF6 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marque: ELK Biotechnology