Dog TIMP1(Tissue Inhibitors Of Metalloproteinase 1) ELISA Kit

Ref. EK-ELK6155

Informations sur le produit

• Nom: Dog TIMP1(Tissue Inhibitors Of Metalloproteinase 1) ELISA Kit
• Synonymes: EPO; CLGI; EPA; HCI; Erythroid Potentiating Activity; Collagenase Inhibitor; Metalloproteinase inhibitor 1; Fibroblast collagenase inhibitor

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dog TIMP1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Dog TIMP1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dog TIMP1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Dog TIMP1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marque: ELK Biotechnology