Rat INSL3(Insulin Like Protein 3) ELISA Kit

Ref. EK-ELK6400

Informations sur le produit

• Nom: Rat INSL3(Insulin Like Protein 3) ELISA Kit
• Synonymes: RLNL; RLF; Relaxin-Like Factor,Leydig Cell; Leydig insulin-like peptide

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat INSL3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat INSL3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat INSL3 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marque: ELK Biotechnology