Cattle NGF(Nerve Growth Factor) ELISA Kit

Ref. EK-ELK7323

Informations sur le produit

• Nom: Cattle NGF(Nerve Growth Factor) ELISA Kit
• Synonymes: NGFB; Beta-NGF; HSAN5; NGF-B; Beta-Nerve Growth Factor

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cattle NGF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Cattle NGF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cattle NGF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cattle NGF in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marque: ELK Biotechnology