8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit

Ref. EK-ELK7861

Informations sur le produit

• Nom: 8-OHdG(8-Hydroxydeoxyguanosine) ELISA Kit
• Synonymes: 8OHdG; 7,8-Dihydro-8-Oxo-2'-Deoxyguanosine; 7,8-Dihydro-8-Oxodeoxyguanosine; 8-Hydroxy-2'-Deoxyguanosine; 8-Oxo-DG; 7,8-dihydrodeoxyguanosine

Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with 8-OHdG protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to 8-OHdG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of 8-OHdG in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marque: ELK Biotechnology