Pig PAG4(Pregnancy associated glycoprotein 4) ELISA Kit

Ref. EK-ELK8657

Informations sur le produit

• Nom: Pig PAG4(Pregnancy associated glycoprotein 4) ELISA Kit
• Synonymes: PAG4; Pregnancy associated glycoprotein 4

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pig PAG4. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Pig PAG4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pig PAG4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pig PAG4 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marque: ELK Biotechnology