Mouse E-Cad(E-Cadherin) ELISA Kit
Ref. EK-ELK9604
| 48T | Arrêté | ||
| 96T | Arrêté |
Informations sur le produit
- CDH1; Arc-1; CD324; CDHE; LCAM; UVO; CAM 120/80; Epithelial Cadherin; Uvomorulin
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse E-Cad. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse E-Cad. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse E-Cad, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse E-Cad in the samples is then determined by comparing the OD of the samples to the standard curve.
