Rat Rock2(Rho Associated Coiled Coil Containing Protein Kinase 2) ELISA Kit

Ref. EK-ELK9932

Informations sur le produit

• Nom: Rat Rock2(Rho Associated Coiled Coil Containing Protein Kinase 2) ELISA Kit
• Synonymes: ROCK-II

Description

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat Rock2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Rock2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat Rock2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Rock2 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Marque: ELK Biotechnology