10X Tris-Glycine-SDS Buffer for molecular biology
Ref. SR-57806
| 200ml | 20,00 € | ||
| 1000ml | 32,00 € |
Informations sur le produit
SDS-PAGE is a method for separating proteins according to their movement in an electrical current and their molecular mass, which relates to the length of their polypeptide chains. A widely recognized system for this technique is the Laemmli system, introduced in 1970. It employs Tris-Glycine gels made up of a stacking gel and a resolving gel, utilizing varying acrylamide concentrations to achieve separation based on molecular weight. This traditional system incorporates a discontinuous buffer setup, with different pH levels and ionic strengths for the buffers: the running buffer (10X Tris-Glycine-SDS Gel Running Buffer) is set at pH 8.3, while the stacking gel operates at pH 6.8, and the resolving gel is at pH 8.8.
Propriétés chimiques
Question d’ordre technique sur : SR-57806 10X Tris-Glycine-SDS Buffer for molecular biology
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