Phosphatase Inhibitor Cocktail III (2 Tubes, 100x)

Ref. TM-C0004

Informations sur le produit

• Nom: Phosphatase Inhibitor Cocktail III (2 Tubes, 100x)

Description

It is easy for the proteins to be degraded or dephosphorylated during in vitro extraction procedure, which could cause an inaccurate result of protein expression detection. Therefore, adding protease inhibitors or phosphatase inhibitors into the extracts would be an effective method to prevent degradation and dephosphorylation of protein. The phosphatase inhibitor mixture can effectively inhibit the dephosphorylation of common phosphatases on proteins and maintain the original phosphorylation state of proteins.

COMPOSITION:

Tube No.: I (ddH2O) 1mL.
Ingredient: Sodium Fluoride.
CAS: 7681-49-4.
MW: 41.99.
Concentration (100x): 100 mM.
Target: Acid Phosphatase.
Type: Reversible.

Tube No.: Sodium Orthovanadate.
Ingredient: 13721-39-6.
CAS: 183.91.
MW: 100 mM.
Concentration (100x): Alkaline phosphatase, PTPs, ATPases.
Target: Reversible.

Tube No.: Sodium Molybdate.
Ingredient: 7631-95-0.
CAS: 205.92.
MW: 115 mM.
Concentration (100x): Acid and phosphoprotein Phosphatase.
Target: Irreversible.

Tube No.: Sodium Tartrate.
Ingredient: 868-18-8.
CAS: 194.05.
MW: 400mM.
Concentration (100x): Acid Phosphatase.
Target: Reversible.

Tube No.: Imidazole.
Ingredient: 288-32-4.
CAS: 68.08.
MW: 200 mM.
Concentration (100x): Alkaline phosphatase.
Target: Reversible.

Tube No.: II (DMSO) 1mL.
Ingredient: (-)-p-Bromotetramisole oxalate.
CAS: 62284-79-1.
MW: 373.22.
Concentration (100x): 2.5 mM.
Target: Alkaline phosphatases.
Type: Irreversible.

Tube No.: Cantharidin.
Ingredient: 56-25-7.
CAS: 196.2.
MW: 500 μM.
Concentration (100x): Ser/Thr phosphatases.
Target: Reversible.

Tube No.: Microcystin LR.
Ingredient: 101043-37-2.
CAS: 995.17.
MW: 500 nM.
Concentration (100x): Acid and PP1 and PP2A.
Target: Reversible.

FEATURES:
Accurate Ingredients -- Clearance of components and concentrations to avoid experimental uncertainty
Comprehensive Protection-- Effectively inhibit various proteases to protect proteins from being degraded
Cost-Effective -- Lower price with higher quality.

HANDLING INSTRUCTION:
Compatible with Western Blot analysis, IP, Co-IP, pull-down, IF, IHC, kinase assay and etc.
Add concentrated cocktail at 1:100 (v/v) dilution to samples solution (such as cell lysates or tissue extracts) before assaying.
Briefly vortex cocktails to help facilitate the dissolution.
Note to add tube I first, mix well, then add tube II, mix again.

NOTICE:
Following initial thaw, aliquot and freeze (-20°C) to avoid thawing and refreezing repeatedly.
If Cocktail I and Cocktail II are used together, do not mix tube I with tube II beforehand, as there may be precipitation. To avoid this, please add them step by step during experiment.

• Marque: Targetmol

Propriétés chimiques

• Couleur/Forme: Liquid