CAS 1158-17-4

o-Nitrofenil β-D-tiogalattoside

Descrizione:

o-Nitrofenil β-D-tiogalattoside (comunemente abbreviato come ONPG) è un composto sintetico ampiamente utilizzato come substrato in saggi biochimici, in particolare nello studio dell'attività enzimatica, come la β-galattosidasi. È un solido cristallino incolore solubile in acqua e solventi organici. L'ONPG è caratterizzato dalla sua capacità di rilasciare o-nitrofenolo dopo idrolisi, che può essere misurato quantitativamente grazie al suo colore giallo, rendendolo un indicatore utile nei saggi enzimatici. Il composto presenta un legame tiogalattosidico, suscettibile a scissione enzimatica, che consente ai ricercatori di studiare la cinetica e i meccanismi delle enzimi glicosidasi. La sua struttura molecolare include un gruppo nitrofenile, che contribuisce alle sue proprietà spettroscopiche. L'ONPG è spesso utilizzato in microbiologia per la rilevazione della fermentazione del lattosio e in biologia molecolare per la valutazione dell'espressione genica. Devono essere osservate precauzioni di sicurezza quando si maneggia l'ONPG, come con molte sostanze chimiche, a causa della potenziale tossicità e dell'impatto ambientale.

Formula: C₁₂H₁₅NO₇S

InChI: InChI=1/C12H15NO7S/c14-5-7-9(15)10(16)11(17)12(20-7)21-8-4-2-1-3-6(8)13(18)19/h1-4,7,9-12,14-17H,5H2

InChI key: InChIKey=SZAOZNVCHHBUDZ-RUXWNWLUSA-N

SMILES: S([C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O)C2=C(N(=O)=O)C=CC=C2

Sinonimi:

• 2-Nitrophenyl 1-Thiohexopyranoside
• 2-Nitrophenyl 1-thio-β-<span class="text-smallcaps">D</span>-galactopyranoside
• 2-Nitrophenyl-β-<span class="text-smallcaps">D</span>-thiogalactopyranoside
• 2-nitrophenyl 1-thio-beta-D-galactopyranoside
• Galactopyranoside, o-nitrophenyl 1-thio-
• Galactopyranoside, o-nitrophenyl 1-thio-, β-<span class="text-smallcaps">D</span>-
• Thio-o-nitrophenyl-β-<span class="text-smallcaps">D</span>-galactopyranoside
• beta-D-Galactopyranoside, 2-nitrophenyl 1-thio-
• o-Nitrophenol beta-thiogalactoside
• o-Nitrophenyl 1-thio-beta-D-galactopyranoside
• o-Nitrophenyl 1-thio-β-<span class="text-smallcaps">D</span>-galactoside
• o-Nitrophenyl β-<span class="text-smallcaps">D</span>-thiogalactoside
• β-<span class="text-smallcaps">D</span>-Galactopyranoside, 2-nitrophenyl 1-thio-
• Thio-o-nitrophenyl-β-D-galactopyranoside
• Galactopyranoside, o-nitrophenyl 1-thio-, β-D-
• 2-Nitrophenyl 1-thio-β-D-galactopyranoside
• β-D-Galactopyranoside, 2-nitrophenyl 1-thio-
• ONP-1-THIO-BETA-D-GAL
• O-NITROPHENYLSULFENYL-1-THIO-BETA-D-GALACTOSIDE
• 2-Nitrophenyl-beta-D-thiogalactopyranoside,98%
• o-Nitrophenylsulfenyl-1-thio-β-D-galactoside
• O-NITROPHENYL-1-THIO-BETA-D-GALACTOSIDE
• 2-Nitrophenylb-D-thiogalactopyranoside
• 1-S-(2-Nitrophenyl)-1-thio-β-D-galactopyranose
• RARECHEM AH BS 0066
• 2-(hydroxymethyl)-6-[(2-nitrophenyl)thio]oxane-3,4,5-triol
• O-NITROPHENYL-1-THIO-BETA-D-GALACTOPYRANOSIDE USP/EP/BP
• O-NITROPHENYL-1-THIO-BETA-D-GALACTOPYRANOSID
• 1-(2-Nitrophenylthio)-1-deoxy-β-D-galactopyranose
• 2-NITROPHENYL-BETA-D-THIOGALACTOPYRANOSIDE
• O-NITROPHENYL-BETA-D-THIOGALACTOPYRANOSIDE
• O-NITROPHENYL 1-THIO-B-D-*GALACTOPYRANOS IDE
• 2-Nitrophenyl β-D-thiogalactopyranoside
• o-Nitrophenyl-1-thio-β-D-galactopyranoside≥ 99% (TLC)

β-D-Galactopyranoside, 2-nitrophenyl 1-thio-

CAS: 1158-17-4

Formula: C₁₂H₁₅NO₇S

Purezza: 98%

Colore e forma: Solid

Peso molecolare: 317.3150

Ref: IN-DA000GWC

2-Nitrophenyl b-D-thiogalactopyranoside

CAS: 1158-17-4

Ref: 7W-GC2622

2-Nitrophenyl 1-thio-β-D-galactopyranoside

CAS: 1158-17-4

2-Nitrophenyl 1-thio-β-D-galactopyranoside

Peso molecolare: 317.32g/mol

Ref: 54-BIN1245

2-Nitrophenyl-β-D-thiogalactopyranoside

CAS: 1158-17-4

2-Nitrophenyl-beta-D-thiogalactopyranoside is a chromogenic substrate used to detect the presence of galactosidase enzymes in various biological and biochemical assays. It consists of a nitrophenyl group attached to a galactose sugar molecule through a thioglycosidic bond. When acted upon by enzymes such as galactosidases, it releases the colored nitrophenyl group, which can be detected using spectrophotometry. This substrate is commonly used in assays for the detection of LacZ activity in bacterial cultures, as lacZ encodes for β-galactosidase.

Formula: C₁₂H₁₅NO₇S

Purezza: Min. 98 Area-%

Colore e forma: Yellow Powder

Peso molecolare: 317.32 g/mol

Ref: 3D-EN03315

2-Nitrophenyl-1-thio-β-D-galactopyranoside

CAS: 1158-17-4

2-Nitrophenyl-1-thio-beta-D-galactopyranoside is a nitroaryl compound that can be synthesized from beta-D-galactopyranosides and nitric acid. It has a molecular conformation that is stabilized by hydrogen bonds, which allows it to form supramolecular assemblies with other molecules. The nitro group in this molecule is responsible for the formation of the nitro group in 1,2,4,5,-tetranitrobenzene.

Formula: C₁₂H₁₅NO₇S

Peso molecolare: 317.32 g/mol

Ref: 3D-N-5100