CAS 142988-22-5

DABCYL-.gamma.-Abu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS

Descrizione:

DABCYL-.gamma.-Abu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS è un composto chimico a base di peptidi che funge da sonda fluorescente, comunemente utilizzata nella ricerca biochimica e biofisica. La struttura presenta un gruppo DABCYL, che funge da quenchers, e un gruppo EDANS, un donatore fluorescente, che consente applicazioni di trasferimento di energia per risonanza di Förster (FRET). Questo composto è caratterizzato dalla sua sequenza specifica di amminoacidi, che include vari residui idrofobici e polari, contribuendo alla sua stabilità e proprietà di interazione nei sistemi biologici. La presenza di DABCYL ed EDANS consente lo studio delle interazioni peptidiche, dei cambiamenti conformazionali e dell'attività enzimatica attraverso misurazioni di fluorescenza. Le sue proprietà uniche lo rendono prezioso in saggi per monitorare l'attività proteolitica e altre interazioni molecolari. Inoltre, il design del composto consente la personalizzazione nelle applicazioni di ricerca, rendendolo uno strumento versatile nei campi della biologia molecolare e della biochimica.

Formula: C₉₀H₁₂₀N₂₂O₁₆S

InChI: InChI=1/C90H120N22O16S/c1-12-53(7)76(87(122)103-71(43-61-46-94-49-98-61)84(119)107-77(55(9)113)79(115)66-35-34-64-65(80(66)129(126,127)128)22-17-23-67(64)96-38-37-92)106-86(121)75(52(5)6)105-83(118)68(40-51(3)4)100-82(117)70(42-60-45-93-48-97-60)101-81(116)69(41-56-20-15-14-16-21-56)102-85(120)73-24-19-39-111(73)90(125)72(44-62-47-95-50-99-62)104-88(123)78(54(8)13-2)112(74(114)25-18-36-91)89(124)57-26-28-58(29-27-57)108-109-59-30-32-63(33-31-59)110(10)11/h14-17,20-23,26-35,45-55,68-73,75-78,96,113H,12-13,18-19,24-25,36-44,91-92H2,1-11H3,(H,93,97)(H,94,98)(H,95,99)(H,100,117)(H,101,116)(H,102,120)(H,103,122)(H,104,123)(H,105,118)(H,106,121)(H,107,119)(H,126,127,128)

SMILES: CCC(C)C(C(=NC(Cc1cnc[nH]1)C(=NC(C(C)O)C(=O)c1ccc2c(cccc2NCCN)c1S(=O)(=O)O)O)O)N=C(C(C(C)C)N=C(C(CC(C)C)N=C(C(Cc1cnc[nH]1)N=C(C(Cc1ccccc1)N=C(C1CCCN1C(=O)C(Cc1cnc[nH]1)N=C(C(C(C)CC)N(C(=O)CCCN)C(=O)c1ccc(cc1)N=Nc1ccc(cc1)N(C)C)O)O)O)O)O)O

Sinonimi:

• 4-(4-Dimethylaminophenylazo)benzoyl-4-aminobutanoyl-isoleucyl-histidyl-prolyl-phenylalanyl-histidyl-leucyl-valyl-isoleucyl-histidyl-threonyl-5-((2-aminoethyl)amino)naphthalene-1-sulfonic acid
• Dabcyl-GABA-ile-his-pro-phe-his-leu-val-ile-his-thr-edans
• Dabgab-ipfit-edans
• N-(4-((4-((4-(Dimethylamino)phenyl)azo)benzoyl)amino)-1-oxobutyl)-L-isoleucyl-L-histidyl-L-prolyl-L-phenylalanyl-L-histidyl-L-leucyl-L-valyl-L-isoleucyl-L-histidyl-N-(2-((5-sulfo-1-naphthalenyl)amino)ethyl)-L-threoninamide
• L-Threoninamide, N-(4-((4-((4-(dimethylamino)phenyl)azo)benzoyl)amino)-1-oxobutyl)-L-isoleucyl-L-histidyl-L-prolyl-L-phenylalanyl-L-histidyl-L-leucyl-L-valyl-L-isoleucyl-L-histidyl-N-(2-((5-sulfo-1-naphthalenyl)amino)ethyl)-

Renin FRET Substrate I

CAS: 142988-22-5

Renin FRET Substrate I, designed for human renin, contains angiotensinogen's N-terminal cleavage site.

Formula: C₉₀H₁₂₀N₂₂O₁₆S

Peso molecolare: 1798.15

Ref: TM-T38900

Dabcyl-γ-Abu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Edans

Prodotto controllato

CAS: 142988-22-5

Dabcyl-γ-Abu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-Edans is an angiotensinogen peptide. It has been used as a substrate for Renin and assayed by fluorescence to study the binding affinity of protease inhibitors. Dabcyl is a fluorescent label that can be used in peptide and biochemicals assays, including fluorescence assay. Dabcyl is soluble in water and has little fluorescence quenching with other compounds, making it ideal for use in these applications.

Formula: C₉₀H₁₂₀N₂₂O₁₆S

Purezza: Min. 95%

Peso molecolare: 1,798.16 g/mol

Ref: 3D-SFQ-3731-PI

DABCYL-gamma-Abu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr-EDANS trifluoroacetate salt

Prodotto controllato

CAS: 142988-22-5

DABCYL-gamma-Abu-Ile-His-Pro-Phe-His-Leu-Val-Ile-His-Thr (DABCYL) is a fluorescent substrate that has been used to study the kinetics of peptide hydrolysis by proteases. It is an amino acid sequence that is present in angiotensinogen, which is a blood protein involved in regulating blood pressure. The DABCYL group on the terminal amino acid of the peptide provides a highly fluorescent molecule that can be excited at wavelengths longer than 400 nm. This fluorophore can also be used as a donor for fluorescence resonance energy transfer (FRET) with other fluorophores, such as EDANS, which has been shown to have high affinity for DABCYL. DABCYL can be used to measure enzyme activity or inhibition and has been found to be sensitive enough to detect changes due to dilutions at concentrations as low as 10 nM.

Formula: C₉₀H₁₂₀N₂₂O₁₆S

Purezza: Min. 95%

Peso molecolare: 1,798.12 g/mol

Ref: 3D-FD110909