CAS 35013-72-0

Estere di N-idrossisuccinimmide di biotina

Descrizione:

Estere di N-idrossisuccinimmide di biotina, con il numero CAS 35013-72-0, è un reagente di bioconjugazione comunemente utilizzato in applicazioni biochimiche, in particolare nella marcatura di proteine e altre biomolecole. Questo composto presenta una parte di biotina, che è una vitamina che gioca un ruolo cruciale nel metabolismo cellulare ed è nota per la sua forte affinità per avidina e streptavidina, rendendola inestimabile in vari saggi e tecniche di purificazione. Il componente N-idrossisuccinimide (NHS) migliora la reattività dell'estere, permettendo di formare legami ammidici stabili con ammine primarie presenti in proteine o peptidi. Questa caratteristica è particolarmente utile per creare proteine biotinilate che possono essere facilmente isolate o rilevate. Estere di N-idrossisuccinimmide di biotina è tipicamente solubile in solventi organici e mostra stabilità in condizioni di stoccaggio appropriate. Le sue applicazioni si estendono a settori come l'immunologia, la biologia molecolare e i sistemi di somministrazione di farmaci, dove la marcatura e il tracciamento precisi delle biomolecole sono essenziali per il successo sperimentale.

Formula: C₁₄H₁₉N₃O₅S

InChI: InChI=1/C14H19N3O5S/c18-10-5-6-11(19)17(10)22-12(20)4-2-1-3-9-13-8(7-23-9)15-14(21)16-13/h8-9,13H,1-7H2,(H2,15,16,21)/t8-,9-,13-/m0/s1

InChI key: InChIKey=YMXHPSHLTSZXKH-RVBZMBCESA-N

SMILES: C(CCCC(ON1C(=O)CCC1=O)=O)[C@H]2[C@@]3([C@@](NC(=O)N3)(CS2)[H])[H]

Sinonimi:

• (+)-Biotinyl-N-hydroxysuccinimide
• (N-Hydroxysuccinimidyl)biotin
• 1-({5-[(3aS,4S,6aR)-2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl]pentanoyl}oxy)pyrrolidine-2,5-dione
• 1-{[5-(2-oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanoyl]oxy}pyrrolidine-2,5-dione
• 1H-Thieno[3,4-d]imidazole, 2,5-pyrrolidinedione deriv.
• 1H-Thieno[3,4-d]imidazole-4-pentanoic acid, hexahydro-2-oxo-, 2,5-dioxo-1-pyrrolidinyl ester, (3aS,4S,6aR)-
• 2,5-Pyrrolidinedione, 1-[[5-(hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl)-1-oxopentyl]oxy]-, [3aS-(3aα,4β,6aα)]-
• 2,5-Pyrrolidinedione, 1-[[5-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]oxy]-
• <span class="text-smallcaps">D</span>-Biotin N-hydroxysuccinimide ester
• Biotin N-hydroxysuccinimide
• Biotin N-hydroxysuccinimidyl ester
• Biotin NHS ester
• Biotin succinimidyl ester
• Biotin-Nhs
• Biotin-OSu
• EZ-Link NHS-Biotin
• Iotin-Nhs
• N-Hydroxysuccinimidobiotin
• N-Succinimidyl <span class="text-smallcaps">D</span>-biotinate
• NHS Biotin
• NSC 345668
• Biotin N-hydroxysuccinimide ester
• D-BIOTIN, SE
• (+)-BIOTINYL-N-SUCCINIMIDYL ESTER
• BIOTIN (LONG ARM) NHS
• NHS-D-BIOTIN
• D-BIOTINYL-OSU
• BIOTIN SE
• BNHS
• D-BIOTIN-N-HYDROXYSUCCINIMIDE ESTER
• D-(+)BIOTIN N-HYDROXYSUCCINIMIDE ESTER
• 5-(2-OXO-HEXAHYDRO-THIENO[3,4-D]IMIDAZOL-6-YL)-PENTANOIC ACID 2,5-DIOXO-PYRROLIDIN-1-YL ESTER
• (+)-BIOTIN N-SUCCINIMIDYL ESTER
• BIOTINYL-N-HYDROXY-SUCCINIMIDE

N-Succinimidyl D-Biotinate

CAS: 35013-72-0

Formula: C₁₄H₁₉N₃O₅S

Purezza: >97.0%(HPLC)(N)

Colore e forma: White to Almost white powder to crystal

Peso molecolare: 341.38

Ref: 3B-S0491

(+)-Biotin N-succinimidyl ester, 98%

CAS: 35013-72-0

In coupling reactions to amino acids, peptides and proteins through primary amines

Formula: C₁₄H₁₉N₃O₅S

Purezza: 98%

Colore e forma: Powder or crystals or crystalline powder, White to cream

Peso molecolare: 341.38

Ref: 02-044771

D-Biotinyl-OSu

CAS: 35013-72-0

The activated biotin derivative Biotin-OSu is used for labeling the amino groups of amino acids, peptides, and proteins. When conducting the biotinylation at pH 6.5, N-terminal amino groups can be selectively tagged in the presence of lysine.

Formula: C₁₄H₁₉N₃O₅S

Purezza: ≥ 97%

Colore e forma: White Powder

Peso molecolare: 341.39

Ref: 01-4014225

2,5-Pyrrolidinedione,1-[[5-[(3aS,4S,6aR)-hexahydro-2-oxo-1H-thieno[3,4-d]imidazol-4-yl]-1-oxopentyl]oxy]-

CAS: 35013-72-0

Formula: C₁₄H₁₉N₃O₅S

Purezza: 98%

Colore e forma: Solid

Peso molecolare: 341.3828

Ref: IN-DA00I6XN

Biotin NHS

CAS: 35013-72-0

Biotin NHS (NHS-Biotin) is an amino reactive biotin reagent used to prepare biotinylated surfaces or polypeptides.

Formula: C₁₄H₁₉N₃O₅S

Purezza: 98.27% - 98.63%

Colore e forma: Powder

Peso molecolare: 341.38

Ref: TM-T14579

Biotin NHS

CAS: 35013-72-0

Biotin NHS

Purezza: ≥98%

Peso molecolare: 341.38g/mol

Ref: 54-BUP15883

(+)-Biotin N-hydroxysuccinimide ester

CAS: 35013-72-0

Formula: C₁₄H₁₉N₃O₅S

Peso molecolare: 341.40

Ref: 7W-GV8230

N-Hydroxysuccinimido biotin

CAS: 35013-72-0

N-Hydroxysuccinimido biotin

Purezza: 98%

Colore e forma: White To Off White Powder

Peso molecolare: 341.38g/mol

Ref: 54-BIB101

Biotin NHS

Prodotto controllato

CAS: 35013-72-0

Stability Moisture Sensitive
Applications Amino reactive biotin reagent for preparing biotinylated surfaces or polypeptides.
References Ngo, T.T., et al.: Applied Biochem. Biotech, 7, 443 (1982), Wojchowski, D.M., et al.: J. Immunol. Methods, 90, 173 (1986);

Formula: C₁₄H₁₉N₃O₅S

Colore e forma: Neat

Peso molecolare: 341.38

Ref: TR-S689500

Biotin succinimidyl ester

CAS: 35013-72-0

Biotinylation reagent for labeling nucleic acids or proteins.

Formula: C₁₄H₁₉N₃O₅S

Purezza: Min. 98 Area-%

Colore e forma: White Off-White Powder

Peso molecolare: 341.38 g/mol

Ref: 3D-FB10303

Biotinyl-OSu

CAS: 35013-72-0

Formula: C₁₄H₁₉N₃O₅S

Purezza: 95%

Colore e forma: Solid, Off-white powder

Peso molecolare: 341.38

Ref: 10-F330592

NHS-Biotin

CAS: 35013-72-0

NHS-Biotin is a biotin analog that can be used as an affinity label for the purification of specific proteins. NHS-Biotin is synthesized by reacting monoclonal antibody with lysine residues and a hydroxyl group, which form a covalent bond known as an amide linkage. The NHS-Biotin can then be covalently attached to the protein of interest. This attachment allows the protein to be purified by passing it over a column of immobilized streptavidin. NHS-Biotin has been shown to bind to actin filaments in humans and uptake peptide hormones in the bloodstream. It was also found that human serum contains chemical reactions with NHS-Biotin, forming a disulfide bond between two cysteine residues on the protein.

Formula: C₂₈H₃₉F₃N₆O₉

Purezza: Min. 95%

Peso molecolare: 660.64 g/mol

Ref: 3D-DPG-5707

Biotin-OSu

CAS: 35013-72-0

Biotin-OSu is a biotin derivative that is used as a fluorescence probe for the detection of DNA binding activity. It has been shown to bind to DNA with high affinity in an experimental model. Biotin-OSu binds to the major groove of DNA and can be used to detect dna binding activities, such as protein-DNA interactions, by forming a ternary complex with the target protein and a fluorophore. This compound has also been shown to react with mouse monoclonal antibodies and human serum proteins, which are transfer reactions. Biotin-OSu has also been used as a coupling agent in peptide hormones and streptavidin polymerase chain reactions (PCR). The presence of disulfide bonds in this compound give it stability but limits its solubility.

Formula: C₁₄H₁₉N₃O₅S

Purezza: Min. 95%

Peso molecolare: 341.39 g/mol

Ref: 3D-BOS-6003-PI