CAS 367-93-1

Isopropil β-D-tiogalattopiranoside

Descrizione:

Isopropil β-D-tiogalattopiranoside (IPTG) è un composto sintetico comunemente usato in biologia molecolare come induttore dell'espressione genica. È un galattoside, specificamente un tiogalattoside, il che significa che contiene un atomo di zolfo al posto dell'ossigeno nella parte di galattosio. L'IPTG è strutturalmente simile al lattosio ed è riconosciuto dal sistema dell'operone lac nei batteri, in particolare Escherichia coli, dove si lega al repressore lac, portando all'attivazione dei geni sotto il controllo del promotore lac. Questa proprietà rende l'IPTG uno strumento prezioso per i ricercatori negli studi di espressione proteica. Il composto è tipicamente fornito come una polvere cristallina bianca ed è solubile in acqua, rendendo facile la preparazione di soluzioni per uso sperimentale. L'IPTG è stabile in una gamma di condizioni, anche se dovrebbe essere conservato in un luogo fresco e asciutto per mantenere la sua integrità. Il suo uso è ampiamente diffuso nella clonazione e nella tecnologia del DNA ricombinante, facilitando la produzione di proteine per applicazioni di ricerca e terapeutiche.

Formula: C₉H₁₈O₅S

InChI: InChI=1S/C9H18O5S/c1-4(2)15-9-8(13)7(12)6(11)5(3-10)14-9/h4-13H,3H2,1-2H3/t5-,6+,7+,8-,9+/m1/s1

InChI key: InChIKey=BPHPUYQFMNQIOC-NXRLNHOXSA-N

SMILES: S(C(C)C)[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O

Sinonimi:

• 1-Isopropylthio-β-D-galactopyranoside
• 1-Methylethyl 1-thio-β-<span class="text-smallcaps">D</span>-galactopyranoside
• Galactopyranoside, isopropyl 1-thio-
• Galactopyranoside, isopropyl 1-thio-, β-<span class="text-smallcaps">D</span>-
• Galactopyranoside, isopropyl 1-thio-, β-D-
• Iptg
• Isopropyl 1-thio-β-<span class="text-smallcaps">D</span>-galactopyranoside
• Isopropyl 1-thio-β-<span class="text-smallcaps">D</span>-galactoside
• Isopropyl 1-thio-β-D-galactopyranoside
• Isopropyl 1-thio-β-D-galactoside
• Isopropyl thio-β-<span class="text-smallcaps">D</span>-galactopyranoside
• Isopropyl thio-β-<span class="text-smallcaps">D</span>-galactoside
• Isopropyl thio-β-D-galactopyranoside
• Isopropyl thio-β-D-galactoside
• Isopropyl β-<span class="text-smallcaps">D</span>-thiogalactopyranoside
• Isopropyl β-D-thiogalactopyranoside
• Isopropyl β-thiogalactopyranoside
• Isopropyl β-thiogalactoside
• Isopropyl-β-<span class="text-smallcaps">D</span>-1-thiogalactopyranoside
• Isopropyl-β-D-thiogalaktopyranosid
• Thioisopropyl β-<span class="text-smallcaps">D</span>-galactopyranoside
• Thioisopropyl β-D-galactopyranoside
• isopropil-β-D-tiogalactopiranosida
• β-<span class="text-smallcaps">D</span>-Galactopyranoside, 1-methylethyl 1-thio-
• Isopropylthio-beta-Thiogalactosidase
• Isopropyl-beta-D-thiogalactoside
• β-D-Galactopyranoside, 1-methylethyl 1-thio-
• Isopropyl β-D-1 Thiogalactopyranoside
• Isopropyl β-D-thiogalactoside
• Isopropyl-1-thio-beta-D-galactopyranoside
• HEMIDIOXANE ADDUCT
• Isopropylthio-beta-galactoside
• Isopropyl thiogalactoside
• 1-Methylethyl 1-thio-β-D-galactopyranoside
• Isopropylthio-B-thiogalactopyranoside
• Isopropyl-beta-D-thiogalactopyranoside, Dioxane Free
• 1-methylethyl 1-thio-D-galactopyranoside

Isopropyl-β-D-thiogalactopyranoside, <5ppm dioxane

CAS: 367-93-1

IPTG is a gratuitous beta-D-galactosidase inducer. In Escherichia coli IPTG functions by binding to the lac repressor and altering its conformation. This inactivation prevents the repressor binding to the operator of the lac operon and thus the repression of the beta-galactosidase coding gene lacZ. IPTG is a commonly used reagent in cloning procedures that require induction of beta-galactosidase activity and is used in conjunction with X-Gal or Lapis-β-D-Gal in blue-white color selection of recombinant bacterial colonies.

Formula: C₉H₁₈O₅S

Purezza: Min. 99.0 Area-%

Peso molecolare: 238.30 g/mol

Ref: 3D-I-8000

Isopropyl-β-D-thiogalactopyranoside, approx. 15.0% dioxane

CAS: 367-93-1

A non-metabolizable allolactose analogue, widely used in molecular biology for overexpression of recombinant proteins from inducible systems under the control of lac promoter. IPTG binds to the LacI repressor and causes its release from the lac operator, allowing gene expression to take place. Present in vectors of pGEX, pGEM-T, pET, pRSET, pMAL class and others.

Formula: C₉H₁₈O₅S

Peso molecolare: 238.30 g/mol

Ref: 3D-I-8050

Isopropyl-β-D-thiogalactopyranoside, <5ppm dioxane, plant origin (ex peach gum)

CAS: 367-93-1

A non-metabolizable allolactose analogue, widely used in molecular biology for overexpression of recombinant proteins from inducible systems under the control of lac promoter. IPTG binds to the LacI repressor and causes its release from the lac operator, allowing gene expression to take place. Present in vectors of pGEX, pGEM-T, pET, pRSET, pMAL class and others.

Formula: C₉H₁₈O₅S

Purezza: Min. 99.0 Area-%

Peso molecolare: 238.30 g/mol

Ref: 3D-I-8070

Isopropyl-B-D-Thiogalactopyranoside (IPTG) (dioxan-free) for MB, 99%

CAS: 367-93-1

Formula: C₉H₁₈O₅S

Purezza: min. 99%

Colore e forma: White, Crystalline powder

Peso molecolare: 238.31

Ref: SR-67208

Isopropyl-B-D-Thiogalactopyranoside (IPTG) (dioxan free), 99%

CAS: 367-93-1

Formula: C₉H₁₈O₅S

Purezza: min. 99%

Colore e forma: White, Crystalline powder

Peso molecolare: 238.31

Ref: SR-54110

IPTG, 1M aqueous solution

CAS: 367-93-1

A non-metabolizable allolactose analogue, widely used in molecular biology for overexpression of recombinant proteins from inducible systems under the control of lac promoter. IPTG binds to the LacI repressor and causes its release from the lac operator, allowing gene expression to take place. Present in vectors of pGEX, pGEM-T, pET, pRSET, pMAL class and others.

Formula: C₉H₁₈O₅S

Purezza: Min. 95%

Colore e forma: Clear Liquid

Peso molecolare: 238.3 g/mol

Ref: 3D-EI184219