CAS 65147-22-0

Z-Phe-Arg-AMC

Descrizione:

Z-Phe-Arg-AMC, noto anche come Z-Fenilalanina-Arginina-7-amido-4-metilcumarina, è un substrato peptidico sintetico comunemente utilizzato nella ricerca biochimica, in particolare nello studio delle proteasi. Questo composto presenta un gruppo protettivo Z (benzyloxycarbonile) sul residuo di fenilalanina, che ne migliora stabilità e solubilità. La presenza del residuo di arginina contribuisce alla sua specificità per alcune enzimi, rendendolo uno strumento prezioso per saggi di attività enzimatica. Il moiety AMC (7-amido-4-metilcumarina) funge da reporter fluorogenico, consentendo la rilevazione della scissione enzimatica tramite fluorescenza. A seguito dell'idrolisi da parte delle proteasi, il gruppo AMC viene rilasciato, portando a un aumento della fluorescenza che può essere misurato quantitativamente. Questa proprietà rende Z-Phe-Arg-AMC particolarmente utile in applicazioni di screening ad alto rendimento e studi cinetici di enzimi proteolitici. In generale, Z-Phe-Arg-AMC è caratterizzato dalla sua origine sintetica, specificità per le proteasi e utilità in saggi basati sulla fluorescenza.

Formula: C₃₃H₃₆N₆O₆

InChI: InChI=1S/C33H36N6O6/c1-21-17-29(40)45-28-19-24(14-15-25(21)28)37-30(41)26(13-8-16-36-32(34)35)38-31(42)27(18-22-9-4-2-5-10-22)39-33(43)44-20-23-11-6-3-7-12-23/h2-7,9-12,14-15,17,19,26-27H,8,13,16,18,20H2,1H3,(H,37,41)(H,38,42)(H,39,43)(H4,34,35,36)/t26-,27-/m0/s1

InChI key: InChIKey=ZZGDDBWFXDMARY-SVBPBHIXSA-N

SMILES: CC=1C=2C(=CC(NC([C@@H](NC([C@H](CC3=CC=CC=C3)NC(OCC4=CC=CC=C4)=O)=O)CCCNC(=N)N)=O)=CC2)OC(=O)C1

Sinonimi:

• 3095V
• 5: PN: WO2004106541 PAGE: 15 claimed protein
• <span class="text-smallcaps">L</smallcap>-Argininamide, N-[(phenylmethoxy)carbonyl]-<smallcap>L</span>-phenylalanyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-
• Benzyloxycarbonylphenylalanylarginine 4-methyl-7-coumarylamide
• Carbobenzoxy-<span class="text-smallcaps">L</smallcap>-phenylalanyl-<smallcap>L</span>-arginine-4-methylcoumaryl-7-amide
• N-[(Phenylmethoxy)carbonyl]-<span class="text-smallcaps">L</smallcap>-phenylalanyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-<smallcap>L</span>-argininamide
• Z-Phe-Arg-AMC
• Z-Phe-Arg-AMC . HCl
• benzyl [(2S)-1-({(2S)-5-[(diaminomethylidene)amino]-2-[(4-methyl-2-oxo-2H-chromen-7-yl)amino]pentanoyl}amino)-1-oxo-3-phenylpropan-2-yl]carbamate
• Carbobenzoxy-L-phenylalanyl-L-arginine-4-methylcoumaryl-7-amide
• N-[(Phenylmethoxy)carbonyl]-L-phenylalanyl-N-(4-methyl-2-oxo-2H-1-benzopyran-7-yl)-L-argininamide

Z-Phe-arg-amc hcl

CAS: 65147-22-0

Formula: C₃₃H₃₆N₆O₆

Purezza: 98%

Peso molecolare: 612.6755

Ref: IN-DA00IBGN

N-CBZ-Phe-Arg-AMC

CAS: 65147-22-0

Z-FR-AMC substrate for serine proteases; cathepsins, kallikrein, plasmin. Substrate: 330/390 nm; Product: 342/441 nm.

Formula: C₃₃H₃₆N₆O₆

Purezza: 98%

Colore e forma: White To Off-White Powder

Peso molecolare: 612.68

Ref: TM-TP1013

Z-Phe-Arg-AMC·HCl

CAS: 65147-22-0

Z-Phe-Arg-AMC·HCl is a fluorescent substrate for proteolytic enzymes. It has been shown to be a potent inhibitor of human immunoglobulin (Ig) secretion and protein synthesis in proximal tubules of the kidney, but this inhibition is not seen in the presence of other proteases. Z-Phe-Arg-AMC·HCl also inhibits the activity of soybean trypsin and hemolytic activity. This compound also has an optimum pH of 7.5 and is characterized by intermolecular hydrogen bonding as well as hydroxyl group interactions with enzyme active sites.

Formula: C₃₃H₃₆N₆O₆·HCl

Purezza: Min. 95%

Peso molecolare: 649.14 g/mol

Ref: 3D-FP47305

Z-Phenylalanylarginine 7-Amido-4-Methylcoumarin Hydrochloride Salt extrapure, 99%

CAS: 65147-22-0

Formula: C₃₃H₃₆N₆O₆·HCl

Peso molecolare: 649.15

Ref: SR-89311

Z-Phe-Arg-AMC

CAS: 65147-22-0

sold as the HCl salt

Formula: C₃₃H₃₆N₆O₆

Purezza: Min. 95%

Peso molecolare: 612.68 g/mol

Ref: 3D-MFR-3095-V