CAS 72088-94-9

Carbossifluoresceina

Descrizione:

Carbossifluoresceina è un colorante fluorescente comunemente utilizzato in applicazioni biologiche e chimiche, in particolare nella microscopia a fluorescenza e nella citometria a flusso. È caratterizzato dalla sua fluorescenza verde brillante, che è dovuta alla sua struttura coniugata che consente un'efficiente assorbimento ed emissione della luce. La sostanza è solubile in acqua ed espone fluorescenza dipendente dal pH, rendendola utile per studiare processi cellulari e dinamiche di membrana. Carbossifluoresceina può essere utilizzato come tracciante in vari saggi, inclusi quelli che misurano la vitalità cellulare, la proliferazione e l'integrità della membrana. La sua capacità di formare coniugati stabili con proteine e altre biomolecole ne aumenta l'utilità negli esperimenti di etichettatura e tracciamento. Inoltre, è spesso impiegato nella sintesi di sonde fluorescenti e nello sviluppo di biosensori. Le considerazioni di sicurezza includono maneggiarlo con cura, come con qualsiasi colorante chimico, per evitare potenziali irritazioni della pelle o degli occhi. In generale, Carbossifluoresceina è uno strumento versatile sia nelle applicazioni di ricerca che diagnostiche grazie alle sue distinte proprietà ottiche e compatibilità con i sistemi biologici.

Formula: C₂₁H₁₂O₇

InChI: InChI=1/2C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21;22-11-2-5-14-17(8-11)27-18-9-12(23)3-6-15(18)21(14)16-7-10(19(24)25)1-4-13(16)20(26)28-21/h2*1-9,22-23H,(H,24,25)

SMILES: c1cc2c(cc1C(=O)O)C(=O)OC12c2ccc(cc2Oc2cc(ccc12)O)O.c1cc2c(cc1C(=O)O)C1(c3ccc(cc3Oc3cc(ccc13)O)O)OC2=O

Sinonimi:

• 3',6'-Dihydroxy-1-Oxo-Spiro[Isobenzofuran-3,9'-Xanthene]-5-Carboxylic Acid
• 3',6'-Dihydroxy-3-Oxo-Spiro[Isobenzofuran-1,9'-Xanthene]-5-Carboxylic Acid
• 3',6'-dihydroxy-3H-spiro[2-benzofuran-1,9'-xanthen]-3-one-acetic acid (1:1)
• 3′,6′-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthene]-ar-carboxylic acid
• 4(5)-Carboxyfluorescein
• 5(6)-Carboxyfluorescein
• 5(6)-Carboxyfluorescein, For Fluorescenc E
• 5(6)-Fam
• 5(6)Carboxy fluorescein
• 5-(And-6)-Carboxyfluorescein
• 9'-[9H]Xanthene]-Ar-Carboxylicacid,3',6'-Dihydroxy-Spiro[Isobenzofuran-1(3H
• C 194
• Carboxyfluorescein
• Fluoresceincarboxylic acid
• Spiro(isobenzofuran-1(3H),9'-(9H)xanthene)-ar-carboxylic acid, 3',6'-dihydroxy-3-oxo-

5(6)-Carboxyfluorescein, mixture of isomers, 97%

CAS: 72088-94-9

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Sci

Formula: C₂₁H₁₂O₇

Purezza: 97%

Colore e forma: Orange to brown or orange-red or orange-brown, Powder or crystalline powder and/or chunks

Peso molecolare: 376.32

Ref: 02-L13439

5(6)-Carboxyfluorescein, mixture of isomers

CAS: 72088-94-9

Formula: C₄₂H₂₄O₁₄

Purezza: 95%

Colore e forma: Solid

Peso molecolare: 752.6316

Ref: IN-DA00IKJ4

5(6)-Carboxyfluorescein

CAS: 72088-94-9

5(6)-Carboxyfluorescein

Formula: ·C₂₁H₁₂O₇·C₂₁H₁₂O₇

Purezza: 98% (nmr) (Typical Value in Batch COA)

Colore e forma: pale red solid

Peso molecolare: 752.63g/mol

Ref: 54-BIC1073

5(6)-Carboxyfluorescein

CAS: 72088-94-9

5(6)-Carboxyfluorescein

Purezza: ≥95%

Peso molecolare: 376.32g/mol

Ref: 54-BUP18955

5(6)-Carboxyfluorescein

CAS: 72088-94-9

5(6)-Carboxyfluorescein (5-(and-6)-Carboxyfluorescein mixed isomers) contains a carboxylic acid that can be used to react with primary amines via carbodiimide

Formula: C₂₁H₁₂O₇

Purezza: 97.57% - 98.85%

Colore e forma: Off-White Solid

Peso molecolare: 376.32

Ref: TM-T18893

5(6)-Carboxyfluorescein

CAS: 72088-94-9

Formula: C₂₁H₁₂O₇

Purezza: ≥ 95%

Colore e forma: Yellow to dark orange powder

Peso molecolare: 376.32

Ref: 7W-GE4671

5(6)-Carboxyfluorescein

Prodotto controllato

CAS: 72088-94-9

Applications 5(6)-FAM, A useful reagent for the preparation of hydrolytically stable fluorescent conjugates and is a useful starting material for the synthesis of other fluorescein-derives reagent.
References Theisen, P., et al.: Tetrahedron Lett., 33, 5033 (1992), Mattingly, P.: Bioconjgate Chem., 3, 430 (1992, Bertrand, R., et al.: Biochemistry, 34, 9500 (1995)

Formula: C₂₁H₁₂O₇

Colore e forma: Dark Brown

Peso molecolare: 752.63

Ref: TR-C178315

5(6)-Carboxyfluorescein - mixture

CAS: 72088-94-9

6-Carboxyfluorescein is a fluorescent dye widely used in PCR methodologies, it is used to label the 5' end of oligonucleotides as a reporter in the presence of a quencher at the 3' end (dual labelled probe). During the amplification process, the dye is cleaved, and the fluorescence increases proportionally with the amount of the specific sequence amplified during the PCR process. The development of the fluorescence signal is therefore specifically related to the amplification of the target sequence. It is used to tag DNA and RNA for in vivo and in vitro applications, as well as for structural studies and RNA-protein interactions.

Formula: C₂₁H₁₂O₇

Purezza: Min. 95%

Colore e forma: Powder

Peso molecolare: 376.32 g/mol

Ref: 3D-FC19772

5(6)-Carboxyfluorescein

CAS: 72088-94-9

5(6)-Carboxyfluorescein is a fluorescent dye that can be used as a building block for the synthesis of more complex compounds. It is also used as a reagent for research and detection in various chemical reactions. 5(6)-Carboxyfluorescein has been shown to react with certain amines, alcohols, and thiols to form useful scaffolds for drug discovery. This compound is also used as an intermediate in the production of pharmaceutical drugs and agrochemicals.

Formula: C₂₁H₁₂O₇

Peso molecolare: 376.33 g/mol

Ref: 3D-C-1825

5(6)-Carboxyfluorescein

CAS: 72088-94-9

M03602 - 5(6)-Carboxyfluorescein

Formula: C₄₂H₂₄O₁₄

Purezza: 95%

Colore e forma: Solid, Orange solid

Peso molecolare: 752.64

Ref: 10-M03602