CAS 76823-03-5

5-carbossifluoresceina

Descrizione:

5-carbossifluoresceina è un colorante fluorescente comunemente usato in varie applicazioni biochimiche e analitiche, in particolare nella microscopia a fluorescenza e nella citometria a flusso. È caratterizzato dalla sua brillante fluorescenza verde, che è attribuita alla sua struttura di xantene. Il composto presenta un gruppo acido carbossilico che ne migliora la solubilità in soluzioni acquose, rendendolo adatto per studi biologici. La sua struttura molecolare consente un legame efficace a proteine e acidi nucleici, facilitando il suo utilizzo come tracciante o marcatore nell'imaging cellulare. Inoltre, 5-carbossifluoresceina è spesso impiegato nella sintesi di sonde fluorescenti e nello sviluppo di saggi per rilevare l'attività enzimatica. Il colorante mostra stabilità in una gamma di condizioni di pH, sebbene la sua fluorescenza possa essere influenzata da fattori ambientali come il pH e la forza ionica. In generale, 5-carbossifluoresceina è uno strumento versatile nel campo della biochimica e della biologia molecolare, consentendo ai ricercatori di visualizzare e quantificare processi biologici con alta sensibilità.

Formula: C₂₁H₁₂O₇

InChI: InChI=1/C21H12O7/c22-11-2-5-15-17(8-11)27-18-9-12(23)3-6-16(18)21(15)14-4-1-10(19(24)25)7-13(14)20(26)28-21/h1-9,22-23H,(H,24,25)

InChI key: InChIKey=NJYVEMPWNAYQQN-UHFFFAOYSA-N

SMILES: O=C1OC2(C=3C1=CC(C(O)=O)=CC3)C=4C(OC=5C2=CC=C(O)C5)=CC(O)=CC4

Sinonimi:

• 3',6'-dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid
• 3′,6′-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylic acid
• 3′,6′-Dihydroxy-3-oxospiro[2-benzofuran-1,9′-xanthene]-5-carboxylic acid
• 3′,6′-Dihydroxy-3-oxospiro[isobenzofuran-1(3H),9′-[9H]xanthene]-5-carboxylic acid
• 5-Fam
• Carboxyfluorescein,5-
• FAM (dye)
• Fam 494/520
• Flamma 456 Carboxylic acid
• Hydroxyfluorescein
• Spiro[isobenzofuran-1(3H),9′-[9H]xanthene]-5-carboxylic acid, 3′,6′-dihydroxy-3-oxo-
• 5-Carboxyfluorescein
• FAM
• 3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5-carboxylic acid
• 5-CARBOXYLUORESCEIN
• 5(6)-FAM
• 5-Carboxyfluorescein (5-FAM)
• 4-(6-HYDROXY-3-OXO-3H-XANTHEN-9-YL)-ISOPHTHALIC ACID
• 3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-5(6)-carboxylic acid
• 4-(6-Hydroxy-3-oxo-3H-xanthen-9-yl)-isophth
• 5-(AND-6)-CARBOXY SNAFL(R)-1
• 4(5)-CARBOXYFLUORESCEIN
• 5-(AND-6)-CARBOXYFLUORESCEIN
• 5-(AND-6)-CARBOXY SNARF(R)-1
• 4-carboxyfluorescein
• 5-(AND-6)-CARBOXY SNAFL(R)-2
• 5-CF

5-Carboxyfluorescein

CAS: 76823-03-5

Formula: C₂₁H₁₂O₇

Purezza: >97.0%(T)(HPLC)

Colore e forma: Light yellow to Brown powder to crystal

Peso molecolare: 376.32

Ref: 3B-C2477

5-Carboxyfluorescein, single isomer, 97%

CAS: 76823-03-5

This Thermo Scientific Chemicals brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Sci

Formula: C₂₁H₁₂O₇

Purezza: 97%

Colore e forma: Powder or crystals or crystalline powder, Yellow to orange

Peso molecolare: 376.32

Ref: 02-H66949

5-Carboxy-fluorescein

CAS: 76823-03-5

Widely used green-fluorescent labeling dye.

Formula: C₂₁H₁₂O₇

Purezza: > 98%

Colore e forma: Yellow Powder

Peso molecolare: 376.32

Ref: 01-4045141

5-FAM

CAS: 76823-03-5

5-FAM, a carboxylic acid, reacts with amines for biomolecule labeling; excites at 488 nm, high fluorescence, solubility, absorbance.

Formula: C₂₁H₁₂O₇

Purezza: 98.28% - 99.32%

Colore e forma: Orange Solid

Peso molecolare: 376.32

Ref: TM-T14050

3',6'-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-5-carboxylic acid

CAS: 76823-03-5

Formula: C₂₁H₁₂O₇

Purezza: 97%

Colore e forma: Solid

Peso molecolare: 376.3158

Ref: IN-DA008P3R

5-Carboxyfluorescein

CAS: 76823-03-5

Formula: C₂₁H₁₂O₇

Purezza: ≥ 99.0%

Colore e forma: Yellow to orange powder

Peso molecolare: 376.3

Ref: 7W-GT3783

5-FAM

CAS: 76823-03-5

5-FAM

Purezza: ≥98%

Peso molecolare: 376.32g/mol

Ref: 54-BUP14627

5-Carboxyfluorescein

CAS: 76823-03-5

5-Carboxyfluorescein

Purezza: 98%

Peso molecolare: 376.32g/mol

Ref: 54-BIC1050

5-Carboxyfluorescein (Technical Grade)

Prodotto controllato

CAS: 76823-03-5

Applications 5-Carboxyfluorescein is a useful reagent for the preparation of hydrolytically stable fluorescent conjugates and is a useful starting material for the synthesis of other fluorescein-derives reagent. Dyes and metabolites. References Theisen, P., et al.: Tetrahedron Lett., 33, 5033 (1992); Mattingly, P.: Bioconjgate Chem., 3, 430 (1992); Bertrand, R., et al.: Biochemistry, 34, 9500 (1995);

Formula: C₂₁H₁₂O₇

Colore e forma: Yellow To Dark Yellow

Peso molecolare: 376.32

Ref: TR-C178320

5-Carboxyfluorescein

CAS: 76823-03-5

5-Carboxyfluorescein (5-FAM) is a self-quenching dye and one of the most common labelling agents for biomolecules, cell staining and localisation studies. The fluorescence is pH dependent, therefore it has been used to measure the internal pH of cells. As it is only membrane permeable to dead cells, it can be used as a staining agent to distinguish viable and non-viable cells. It is also used in PCR to label the 5’ end of oligonucleotides in the presence of a quencher at the 3’ end (dual labelled probe). During the amplification process, the dye is cleaved and the fluorescence increases proportionally with the amount of the specific sequence amplified during the PCR process. The development of the fluorescence signal is therefore specifically related to the amplification of the target sequence. The carboxylic acid can be activated to react with a primary amine.

Formula: C₂₁H₁₂O₇

Purezza: Min. 98 Area-%

Colore e forma: Yellow Powder

Peso molecolare: 376.32 g/mol

Ref: 3D-FC19773

3′,6′-Dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9′-xanthene]-5-carboxylic acid

CAS: 76823-03-5

Formula: C₂₁H₁₂O₇

Purezza: 95%

Colore e forma: Solid

Peso molecolare: 376.32

Ref: 10-F448180

5-Carboxyfluorescein

CAS: 76823-03-5

Formula: C₂₁H₁₂O₇

Peso molecolare: 376.32

Ref: ST-EA-CP-F116029