Enzima

Bupicomide is a dopamine β-hydroxylase inhibitor with antihypertensive and vasodilatory activity and may be used in the study of hypertension.

<p>Superoxide dismutase is an enzyme that catalyzes the conversion of harmful superoxide into hydrogen peroxide and oxygen.</p>

Proteinase K is used for the general digestion of proteins and removal of protein contamination in nucleic acids. Addition of Protease K also stabilizes nucleic acids by degrading any nucleases present. Proteinase K is active in wide range of pH range, in the presence of SDS, urea and Guanidinium chloride at low to moderate concentrations. Proteinase K is also known under names of protease K and endopeptidase K.

Myokinase (Adenylate kinase, EC 2.7.4.3) catalyzes interconversion between ATP, ADP and AMP by catalyzing the following reaction:ATP + AMP ⇌ 2 ADPOne unit of Myokinase will convert 1.0 µmol ATP and 1.0 µmol AMP to 2.0 µmol ADP per min at 25°C and pH 7.5.

<p>E. coli recombinant α-2,6 sialyltransferase from Neisseria meningitidis. One unit is defined as the amount of enzyme that catalyses the formation of 1 μmol CMP-Neu5Ac from CTP and Neu5Ac per minute at 37 ºC.Activity: 100U/mg</p>

Tyrosinase-IN-2, a potent tyrosinase inhibitor, may help in skin lightening and food preservation research.

<p>Glucose Dehydrogenase is an enzyme, which is typically derived from microbial sources such as bacteria and fungi. It functions by catalyzing the oxidation of glucose to gluconolactone, concurrently reducing a cofactor such as NAD⁺ or PQQ. This biochemical reaction is critical in various analytical applications due to its specificity and efficiency in glucose detection.Glucose Dehydrogenase is widely employed in the development of biosensors and diagnostic assays. Its primary application is in blood glucose monitoring devices, where its ability to accurately quantify glucose levels is crucial for managing diabetes. Additionally, it is utilized in research and development settings for biochemical assays that require precise glucose measurements. The enzyme's rapid and specific action on glucose molecules makes it an indispensable tool in both clinical and laboratory environments, contributing to advancements in biosensing technologies and metabolic studies.</p>

Carboxypeptidase Y (EC 3.4.16.1) is an exopeptidase enzyme. It hydrolyzes peptide bonds of C-terminal residues and it remains active in the presence of urea at low to moderate concentrations. One unit of the Carboxypeptidase Y will hydrolyze 1.0 μmole of a chromogenic peptide substrate, releasing C-terminal alanine and generating a light-absorbing product.

α-Glucosidase is a glycoside hydrolase enzyme that hydrolyzes α-1,4-linked D-glucose residues to produce α-D-glucose. This enzyme has been isolated from Bacillus stearothermophilus and is used as an industrial catalyst in the production of glucose syrups.  One Unit of α-Glucosidase will release 1.0 µmole of p-nitrophenol from the chromogenic substrate mimic 4-nitrophenyl α-D-glucopyranoside per minute under optimum conditions.

Lyticase is a lysing enzyme that is designed to lyse cells in a biological sample. It contains an optimized wild-type guanine nucleotide-binding protein and has been shown to have high enzyme activities. Lyticase has also been shown to be active against opportunistic fungal strains, such as Candida glabrata, by disrupting their cell membranes. Lyticase is classified as a signal peptide with nuclear DNA, which allows it to be used in wastewater treatment applications. The enzyme can also be used for the analysis of the Toll-like receptor (TLR) response of microbes due to its electrochemical impedance spectroscopy properties.

Lysozyme is a bacteriolytic enzyme, which is primarily derived from hen egg whites. It functions by hydrolyzing the β-1,4-glycosidic linkages in the peptidoglycan layer of bacterial cell walls, particularly in Gram-positive bacteria. This enzymatic activity results in the lysis and subsequent death of the bacterial cells, providing a potent antimicrobial effect.

<p>hCAI/II-IN-6 is a selective and orally active inhibitor of human carbonic anhydrase (CA).</p>

<p>hCAII-IN-9 inhibits hCA II/IX/XII with IC50s of 1.18, 0.17, 2.99 μM; not BBB permeable.</p>

<p>Glucosidases are enzymes belonging to the family of oxidoreductases. They catalyse the hydrolysis of starches to simple sugars. Glucosidase is widely used in the food, carbohydrate and biofuels industries. In recent years, its applicability has expanded to biotechnology for its potential application in bioenzymatic fuel cells.</p>

selectively deamidates gluten peptides, which results in strongly enhanced T cell-stimulatory activity.  It has also been used in a study to improve quantifiable assays to fully characterize the role of transglutaminase in diseases such as Huntington′s disease and Alzheimer′s disease.

<p>Endoproteinase Glu-C (Glutamyl endopeptidase, V8 protease, GluV8, EC 3.4.21.19) is a protease that hydrolyzes peptide bonds at the carboxylic side of either exclusively Glu, or Glu and Asp residues, depending on the buffer conditions. One unit of endoproteinase Glu-C will generate 1.0 μmole of p-nitroaniline from Z-Phe-Leu-Glu-pNA peptide mimic substrate per minute at pH 7.8 and 25 °C. Z-Phe-Leu-Glu-pNA substrate is available here.molecular weight ~ 27000.</p>

Endo-β-1,4-Mannanase (other names Mannan endo-1,4-β-mannosidase, endo-β-1,4-mannase, β-mannanase B, β-1, 4-mannan 4-mannanohydrolase, endo-β-mannanase, β-D-mannanase, 1,4-β-D-mannan mannanohydrolase; EC 3.2.1.78) is an enzyme, catalyzing the hydrolysis of -1, 4-mannosidic linkages in mannans, glucomannans and galactomannans. One unit of Endo-β-1,4-Mannanase will release 1.0 µmole of mannose reducing-sugar per minute from a 3mg/ml mannan solution at pH 5.5 and 37degC.  Expressed in U/g.

<p>Acetylcholinesterase is an enzyme that breaks down acetylcholine</p>

<p>From pigeon breast muscle - Carnitine acetyltransferase (EC 2.3.1.7, also Carnitine O-acetyltransferase) is an enzyme that catalyzes the following chemical reaction: acetyl-CoA + carnitine ⇌ CoA + acetylcarnitine</p>

N-Desethyl Brinzolamide oxalate functions as a dual inhibitor targeting Carbonic anhydrase II and Carbonic anhydrase IV, exhibiting inhibitory concentrations (IC50) of 1.28 nM and 128 nM, respectively [1].

CAXII-IN-1, antitumor, selectively inhibits CA XII with Ki of 3.8 nM for hCA XII and 56 nM for hCA IX.

LX-1031 is an effective inhibitor of tryptophan 5-hydroxylase. LX-1031 decreases serotonin (5-HT) synthesis peripherally.

CA Inhibitor 12 strongly blocks CA II (K_i 1.72 nM), also inhibits CA I (271 nM), shows anticancer effects.

<p>Compound 1e, also known as ALP/Carbonic anhydrase-IN-1, is a dual inhibitor targeting both carbonic anhydrase (CA) isozymes II, IX, and XII, as well as alkaline</p>

Sezolamide hydrochloride is a potent topical carbonic anhydrase inhibitor.

Sezolamide is a carbonic anhydrase inhibitor.

Diethyl-pythiDC is an collagen prolyl 4-hydroxylase inhibitor.

hCAIX-IN-15 is a potent inhibitor of human carbonic anhydrase IX (hCA IX) with an inhibition constant (Ki) of 38.8 nM, exhibiting broad-spectrum anticancer

hCAIX-IN-16 (Compound 12d), an inhibitor of hCA IX, exhibits inhibition constants (K i) of 190.0 nM for hCA IX and 187.9 nM for hCA XII.

hCAIX/XII-IN-8 (compound 3g) is a potent inhibitor of the human carbonic anhydrases (CAs) IX and XII, with inhibition constants (K i) of 8.5 nM for CA IX and 6.

COX-2-IN-30, a benzenesulfonamide derivative, is an orally active, dual inhibitor of cyclooxygenase-2 (COX-2; IC50 = 49 nM) and cyclooxygenase-1 (COX-1; IC50 =

<p>Cholesterol oxidase (EC 1.1.3.6) is an enzyme that catalyzes the following reaction: cholesterol + O2 ⇌ cholest-4-en-3-one + H2O2One unit of cholesterol oxidase will convert 1.0 μmole of cholesterol into cholest-4-en-3-one per min at pH 7.5 and 25 °C. You can remove the build-up of hydrogen peroxide using catalase.</p>

Thioredoxin reductase (TR, TrxR) (EC 1.8.1.9) is an enzyme that reduce thioredoxin using NADPH as a co-factor, and also contains FAD. One unit of thioredoxin reductase will raise increase light absorbance by 1.0 per minute at 412nm in the presence of thioredoxin and Ellman's reagent at pH 7.0 and 25 °C.

Glycerol-3-phosphate oxidase (EC 1.1.3.21) is an enzyme that catalyzes the following reaction:  glycerol-3-phosphate + O2 ⇌ dihydroxyacetone phosphate + H2O2  One unit of Glycerol-3-phosphate oxidase will generate 1.0 μmole H2O2 per min at 37°C, under the presence of O2 and the optimal pH. If required, you can remove the build-up of hydrogen peroxide using catalase.

Glycerokinase (glycerol kinase, GP, ATP-glycerol 3-phosphotransferase; EC 2.7.1.30) is an enzyme that catalyzes the following reaction:  ATP + glycerol ⇌ ADP + glycerol 3-phosphate  One unit of Glycerokinase will convert 1.0 μmole of glycerol and ATP to glycerol 3-phosphate and ADP per min at pH 9.8 and 25 °C.

<p>Sulfatase from Helix pomatia is a highly potent enzyme that is capable of hydrolyzing sulfated compounds and sulfate esters. It has been widely used in various applications such as glucosinolate analysis, genistein extraction preparation, and regiospecificity studies. With a concentration of ≥10,000 units per gram of solid, this sulfatase offers exceptional enzymatic activity for sulfatase assays. It effectively catalyzes the hydrolysis of sulfated substrates, including p-nitrocatechol sulfate, naphthyl sulfate and phenyl sulfates.The enzyme can be incubated with the desired sample to facilitate the release of sulfate groups from sulfated compounds. Sulfatase from Helix pomatia is a valuable tool for researchers and scientists working in diverse fields requiring efficient and reliable enzymatic hydrolysis capabilities. Additionally the enzyme has been found to have industrial applications, such as in the bioconversion of industrial chemicals, where it can be used as a catalyst.</p>

<p>Triose-phosphate isomerase (TPI, TIM; EC 5.3.1.1) is an enzyme that catalyzes the reversible isomerisation of dihydroxyacetone phosphate and D-glyceraldehyde 3-phosphate:  DHAP ⇌ GADP  The reaction mechanism involves the formation of an enediol intermediate. One unit of Triose-phosphate isomerase will convert 1.0 μmole glyceraldehyde 3-phosphate to dihydroxyacetone phosphate per min at pH 7.6 and 25 °C.</p>

p-Ethynylphenylalanine (4-Ethynyl-L-phenylalanine), a tryptophan hydroxylase (TPH) inhibitor, is competitive, effective, selective, and reversible, with a Ki of

Phosphodiesterase II from bovine spleen is an enzyme derived from the spleen of cattle, which serves as a crucial biological catalyst for the hydrolysis of phosphodiester bonds in nucleotide sequences. This enzyme's mode of action involves cleaving the phosphodiester linkages within nucleic acids, facilitating the breakdown of these macromolecules into smaller nucleotide units.

<p>Oxalate Oxidase, freeze-dried, is an enzymatic preparation that serves as a catalyst in biochemical reactions. This enzyme is derived from wheat, a common plant source, ensuring a naturally occurring origin. Its primary mode of action is the oxidation of oxalate into carbon dioxide and hydrogen peroxide. This biochemical activity is significant in various scientific applications, specifically in the breakdown of oxalate, which plays a crucial role in metabolic and environmental processes.</p>

<p>Sarcosine oxidase (Monomeric sarcosine oxidase, MSOX, EC 1.5.3.1) is an enzyme that catalyzes the oxidative demethylation of sarcosine to yield glycine, H2O2 and formaldehyde in the following reaction:  CH3-NH2+-CH2-COO- + H2O + O2 → NH3+-CH2-COO- + H2O2 + CH2O or sarcosine + water + oxygen → glycine + hydrogen peroxyde + formaldehydeOne unit of Sarcosine oxidase will form 1.0 micromole of formaldehyde from sarcosine per minute at pH 8.3 and 37 °C. You can remove the build-up of hydrogen peroxide using catalase.</p>

<p>Poly(ethylene terephthalate) hydrolase is an enzyme involved in the breakdown of Polyethylene terephthalate which is present in many plastics Polyethylene terephthalate hydrolytic enzymes may be useful in biotechnology, for use in waste treatment, biocatalysis and biorecycling</p>

Tyrosinase-IN-37 (Compound 3c) is a potent inhibitor of tyrosinase, with an IC50 value of 1.02 μM, which is 14 times more effective than kojic acid (IC50 of 14.74 μM). This compound effectively prevents the browning of Rosa roxburghii and can also inhibit browning not caused by tyrosinase.

β-Glucuronidase/hCAII-IN-2 is a potent inhibitor of β-glucuronidase and hCA II, and their IC50 values were 670.7 μM and 21.77 μM, respectively.

Perfluorohexane sulfonamide (FHxSA) serves as an inhibitor of carbonic anhydrase (CA), effectively inhibiting bovine CA and human CAII with IC50 values of 0.122 and 1.38 μM, respectively. Additionally, it acts as a delayed-action insecticide for controlling red imported fire ants (Solenopsis invicta). Furthermore, Perfluorohexane sulfonamide is considered a potential environmental pollutant.

AChE/CA I-IN-1 (Compound 2g) acts as an inhibitor for both AChE and hCA I, with Ki values of 1.85 µM and 0.53 µM, respectively. It has shown potential applications in the research of Alzheimer's disease, glaucoma, and epilepsy.

Isobutylamido thiazolyl resorcinol is a tyrosinase (Tyrosinase) inhibitor that prevents pigment deposition induced by ultraviolet radiation.

<p>hCAII-IN-3 inhibits key hCA isoforms with Ki: hCA I (403.8 nM), hCA II (5.1 nM), hCA IX (10.2 nM), hCA XII (5.2 nM); shows anticancer potential.</p>

Sulclamide, a sulfamoylbenzoic acid derivative, exhibits diuretic activity and functions as an inhibitor of carbonic anhydrase [1].

CAII-IN-3, a thiosemicarbazone, potently inhibits CA-II with an IC50 of 13.4 μM.

Tyrosinase-IN-33 (compound 5), a pyridine-based compound, acts as an effective inhibitor of diphenolase activity in mushroom tyrosinase. It significantly reduces enzyme activity with an IC50 of 9.0 μM.

Tyrosinase-IN-35 (compound 6g), exhibiting a IC 50 value of 2.09 μM, serves as a more effective inhibitor of human tyrosinase compared to Kojic Acid (IC 50: 16.38 μM). At concentrations of 4 μM and 8 μM, this compound effectively lowers melanin levels in melanoma B16F10 cells in vitro.

Carbonic anhydrase inhibitor 16 (compound 1) is a CA I/CA II inhibitor with potential antiviral activity, used in virus infection studies.

α-Glucosidase-IN-63 (Compound 4d) serves as an α-Glucosidase inhibitor with an IC 50 value of 0.44 μM. Additionally, it exhibits inhibitory activity against hCA II, demonstrating a K i of 7.0 nM. The compound is also effective when administered orally. [1]

Carbonic anhydrase inhibitor19 (compound 26a) targets glaucoma-associated isozymes hCA II and hCA XII, with inhibition constants (Kis) of 9.4 nM and 6.7 nM, respectively. This compound is effective in reducing intraocular pressure.

CAIX-IN-3 (Compound 27) is a selective and potent inhibitor of carbonic anhydrase IX (CAIX), with an IC50 of 0.48 nM.

Carbonic anhydrase inhibitor 17 (compound 7c) is a pyrazine-based sulfonamide that acts as a carbonic anhydrase II inhibitor, with an IC50 value of 0.63 nM.

<p>hCAI/II/XII-IN-1 (compound 7) is an inhibitor of human carbonic anhydrases hCAI, hCAII, and hCAXII, with Ki values of 78.5 nM, 9.1 nM, 605 nM, 7.7 nM, and 3.7 nM.</p>

Compound 7c inhibits carbonic anhydrase II, lowering intraocular pressure in glaucomatous rabbits.

Tyrosinase-IN-29 (compound 5c) is an effective inhibitor of abTYR tyrosinase, demonstrating an IC50 value of 6.11 μM. It is suitable for further research into the inhibition of excessive skin pigmentation.

GSK 366 is a potent kynurenine-3-monooxygenase (KMO) inhibitor (IC50s: 0.7 nM and 2.3 nM for P. fluorescens-KMO and human KMO).

EGFR/VEGFR2-IN-2 (compound 4b) serves as a dual inhibitor of VEGFR-2 and EGFR.

hCAIX/XII-IN-15 (Compound 17β) is an inhibitor of hCA IX and hCA XII, exhibiting Ki values of 0.42 and 4.37 μM, respectively. It demonstrates a pro-apoptotic effect in multiple myeloma cells.

hCAVII/IX-IN-1 (compound 4) functions as an inhibitor of hCAVII/IX, exhibiting Ki values of 56.5 nM and 38.2 nM, respectively. It is applicable in the field of cancer research.

hCA XII/II/IX-IN-1 inhibits hCA I/II/IX/XII (IC50: 2.6, 0.004, 0.005, 0.001 μM) with anticancer properties.

hCAIX-IN-20 (compound APBS-5m) is a potent inhibitor of carbonic anhydrase IX (hCA IX), with a Ki of 2.7 nM, playing a significant role in cancer research.

R-13, a carbonic anhydrase inhibitor, has Ki of 60.7 nM (hCA I), 320.7 nM (hCA II), and 2298 nM (hCA IV).

Potent hCA inhibitor: targets hCA II, IX & XII with IC50s of 42.9, 47.6, & 6.7 nM respectively.

O-Desmethyl Brinzolamide hydrochloride (compound 6a), a potent metabolite derived from Brinzolamide, serves as a carbonic anhydrase (CA) inhibitor. It exhibits a dissociation constant (Kd) of 0.136 nM for CA II and an inhibitory concentration (IC50) of 165 nM for CA IV [1].

Carbonic anhydrase inhibitor 9 targets hCA II and IX with Ki of 56.4 and 56.9 nM respectively; shows antiproliferative activity.

hCA VB-IN-1 (compound 15) is a potent and selective inhibitor of hCA VB (carbonic anhydrase) with a KI of 515.7 nM [1].

hCAII-IN-5 (compound 12h) is a potent, selective inhibitor of human carbonic anhydrase II (hCA II) with an inhibition constant (IC50) of 4.55 µM [1].

CAII-IN-2 (3g): potent, selective CA-II inhibitor; IC50-12.1 μM for bovine CA-II; valuable in CA-related disorder research.

CA inhibitor 10 targets MCF-7 cells, IC50: 11.9 μM; potent h CA IX inhibitor, Ki: 6.2 nM. Anti-cancer research.

4-Acetylphenylboronic acid acts as an effective inhibitor targeting carbonic anhydrase II (CAII), displaying inhibitory concentrations (IC50) of 246 μM for bovine CAII (bCA II) and 281.40 μM for human CAII (hCA II).

hCAII-IN-4 (Compound 12j) is a potent inhibitor of human carbonic anhydrase II (hCA II), exhibiting an inhibitory concentration (IC50) of 7.78 μM.

β-Glucuronidase/hCAII-IN-2 (Compound 12e) is a compound that effectively inhibits both β-glucuronidase and human Carbonic Anhydrase II (hCA II), exhibiting IC50

NSC 828467 is one of the top five CA-IX inhibitors with significant in vitro anticancer activity (IC50: 27.2 nM).

<p>Valerate sodium is a potential Carbonic anhydrase 1/2 inhibitor for biochemical experiments.</p>

CAII-IN-1 (3n) is a selective bovine CA-II inhibitor with 10.3 μM IC50, used in carbonic anhydrase disorder studies.

<p>Tyrosinase-IN-20 (compound 6a) acts as a potent Tyrosinase inhibitor, demonstrating an IC 50 value of 28.50 μM [1].</p>

Plodicitinib is an inhibitor of Janus tyrosine kinase 3/TEC family kinase, exhibiting anti-inflammatory properties.

CA inhibitor 4, photoprobe; targets hCA I-XIV; Ki: 640-1166 nM.

Carbonic anhydrase inhibitor 3 (compound 11g) is an inhibitor of carbonic anhydrase II that reduces the intraocular pressure in glaucomatous rabbits [1].

CA IX-IN-1 (compound 12g) is a potent and highly selective hCA IX inhibitor (IC50: 7 nM) that exhibits antitumour effects.

One unit of alkaline phosphatase (EC 3.1.3.1) will hydrolyze 1.0 µmol of 4-nitrophenyl phosphate per min at 25°C and pH 9.6.

<p>Expression system: HEK297 Cells&lt;br&gt;Length: 21-472, Full Length of Mature Protein&lt;br&gt;Activity: Not Tested</p>

<p>Expression system: E. coli&lt;br&gt;Length: 1-169, Partial&lt;br&gt;Activity: BLI</p>

<p>VEGFR3/FLT4 Protein, Human, Recombinant (His &amp; Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag.</p>

<p>ACE2/ACEH Protein, Human, Recombinant (hFc), Biotinylated is expressed in HEK293 mammalian cells with hFc tag.</p>

<p>Protein phosphatase 1G, also known as Protein phosphatase 1C, Protein phosphatase 2C isoform gamma, Protein phosphatase magnesium-dependent 1 gamma, PP2C-gamma</p>

<p>Cyclophilin A Protein, Human, Recombinant (His) is expressed in E.</p>

<p>Fructose-2,6-biphosphatase 3, also known as 6-phosphofructo-2-kinase or PFK2 or PFKFB3, is a potent activator of phosphofructokinase, which is a rate-limiting</p>

<p>Carbonic Anhydrase 9 Protein, Human, Recombinant (His &amp; Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag.</p>

<p>Carboxypeptidase B2 Protein, Human, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.</p>

<p>Genetic engineers have used glutathione S-transferase to create the GST gene fusion system.</p>

<p>GUCY2C Protein, Canine, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.</p>

<p>Protein kinase C iota type, also known as Atypical protein kinase C-lambda/iota, aPKC-lambda/iota and PRKCI, is a cytoplasm, membrane and nucleus protein which</p>

<p>GUCY2C Protein, Cynomolgus, Recombinant (His) is expressed in HEK293 mammalian cells with His tag.</p>

<p>Penicillin G acylase, 10mg/ml aqueous solution, is a biocatalytic enzyme used in pharmaceutical research and production. This enzyme is typically sourced from various microbial species, predominantly bacteria, through fermentation processes. Its primary mode of action involves the hydrolysis of penicillin G, catalyzing the cleavage of the side chain amide bond to produce 6-aminopenicillanic acid (6-APA), a core building block for the synthesis of a variety of semi-synthetic penicillins.</p>