Altri

<p>Modified naphtol AS substrate used in histochemical and cytochemical staining</p>

<p>5-Bromo-6-chloro-3-indoxylphosphate, disodium salt trihydrate is a bioluminescent substrate that can be used in a variety of diagnostic applications. This compound reacts with luciferase to yield light and can serve as an indicator for various enzymes and chemical reactions. 5-Bromo-6-chloro-3-indoxylphosphate, disodium salt trihydrate has been shown to be compatible with both culture media and food testing.</p>

<p>Chromogenic substrate for esterases</p>

<p>6-Chloro-3-indoxyl caprylate is a high quality, enzyme substrate, chromogenic substrate, bioluminescence, chemiluminescence ligand, staining and diagnostics reagent. It has CAS No. 159954-35-5 and is used in food testing, culture media and environmental testing. 6-Chloro-3-indoxyl caprylate is also a fluorogenic substrate that is used for high purity applications such as conjugation with other molecules.</p>

<p>5-Bromo-6-chloro-3-indolyl α-D-glucopyranoside is a chromogenic substrate that yields a magenta-colored product after cleavage. The chromophore 5-bromo-6-chloro-indoxyl is released, generating the observable color. It is used in Pompe disease diagnostics, a glycogen storage disorder, that measures the deffiency of alpha-glucosidase (GAA) that could lead to the accumulation of glycogen in lysosomes.</p>

<p>Aldol® 495 nonanoate is a chromogenic and fluorogenic enzyme substrate used for detection of lipase activity. The colorless enzyme substrate is cleaved by C9 esterases, yielding development of an orange coloration. In addition, green fluorescence is generated when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 495 nonanoate can be used in enzyme assays and live bacterial cultures. Aldol® 495 nonanoate is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.</p>

<p>Chromogenic substrate yielding a soluble yellow chromophor for the colorimetric assay of phosphatases.</p>

<p>Aldol® 484 alpha-D-glucopyranoside is a chromogenic and fluorogenic enzyme substrate for ?-glucosidase activity (EC 3.2.1.20, ?-1,4-glucosidase, also known as maltase). The colorless enzyme substrate is cleaved by bacteria producing this enzyme in liquid or solid media, yielding orange color. Green fluorescence is also generated if a suitable matrix is present such as: cellulose acetate filters, polypropylene tubes or microplates and polyethylene vials. Aldol® 484 alpha-D-glucopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>5-Bromo-6-chloro-3-indolyl acetate is a chromogenic substrate that yields a magenta-colored product after cleavage. The chromophore 5-bromo-6-chloro-indoxyl is released, generating the observable color. It is used in bacterial esterase detection (e.g. Staphylococcus spp.).</p>

<p>1-Naphthyl beta-D-glucuronide (NPG) is a chromogenic enzyme substrate used to detect the presence of β-glucuronidase activity. Upon hydrolysis by the enzyme, it produces a yellow product that can be detected by spectrophotometry or visually. It is commonly used in clinical assays to detect the presence of the enzyme in urine samples, as elevated levels can indicate liver or urinary tract diseases. It is also utilized in environmental testing, as some bacteria strains can produce the enzyme as a by-product, making NPG a useful tool for detecting these strains in contaminated water or food sources.</p>

<p>Chlorophenol red-b-D-galactopyranoside sodium salt is the salt from of CPRG (chlorophenol red-b-D-galactopyranoside), that has a higher solubility. It is a chromogenic substrate for beta-galactosidases that, after enzymatic cleavage, releases chlorophenol red, a dark red compound quantified by absorbance at 570 nm.</p>

<p>Modified naphtol AS substrate used in histochemical and cytochemical staining</p>

<p>2,4-Dicyano-3-(3,4-dihydroxy-5-nitrophenyl)-N1,N1,N5,N5-tetraethylpentanediamide is a white crystalline solid that is used as an analytical reference standard in HPLC. It is used for the determination of purity and impurities in pharmaceuticals and drug products. This compound can be custom synthesized or obtained from natural sources. It can be found in the USP Drug Impurity Standards and the European Pharmacopoeia.</p>

<p>3-Indoxyl caprylate is an enzyme substrate used in a variety of diagnostic applications, including the detection of various bacterial and viral infections. It is used as a substrate for beta-galactosidase, which can be detected by bioluminescence or chromogenic substrates. 3-Indoxyl caprylate also serves as a ligand for receptor binding and has been shown to bind to CD4 receptors on human T cells. 3-Indoxyl caprylate is also used as an enzyme substrate in the detection of DNA polymerases, RNA polymerases, and ATPases. This product is available with a high purity that ranges from 98% to 99%. 3-Indoxyl caprylate is soluble in water, alcohols, acetone, acetic acid, and chloroform.</p>

<p>Aldol® 518 beta-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-galactosidase activity. The colorless enzyme substrate is cleaved by lactose-utilizing bacteria in liquid or solid media, yielding intense red color as well as red fluorescence when a fluorescence enhancer or a suitable matrix is present. Aldol® 518 beta-D-galactopyranoside can also be used in beta-gal reporter gene assays or for identification of beta-gal positive transformants on agar plates. Aldol® 518 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>Chromogenic β-Glucosaminidase substrate yielding a yellow absorbent solution upon cleavage.</p>

<p>4-Nitrophenyl beta-D-xylobioside is a chromogenic substrate for xylanase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl beta-D-xylobioside is used in different applications such as the Xylan degradation studies, paper/pulp industry applications</p>

<p>Substrate detecting beta-glucosidase activity</p>

<p>Aldol® 515 caprylate is a chromogenic and fluorogenic enzyme substrate used for detection of lipase activity. The colorless enzyme substrate is cleaved by C8 esterases, yielding development of red coloration. In addition, red fluorescence is generated when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 515 caprylate can be used in enzyme assays and live bacterial cultures. Aldol® 515 caprylate is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.</p>

<p>2-O-(4-Nitrophenyl)-a-D-N-glycolylneuraminic acid is commonly used to measure the activity of sialidase enzymes, specifically those that cleave glycoconjugates containing 2-O-(4-nitrophenyl)-alpha-D-N-glycolylneuraminic acid. The resulting product can be detected by measuring the absorbance at a specific wavelength using a spectrophotometer. This substrate offers a high degree of specificity for glycolylneuraminic acid-containing substrates and is useful for both research and diagnostic purposes.</p>

<p>The maunfacturing of blue jeans started 150 years ago as workwear, and are found nowadays in almost every wardrobe. Their renowned indigo color tone is truly unique; nevertheless, the high demand for the dye poses serious sustainability and environmental problems connected with its manufacturing process. Recently, a group of scientists presented an alternative indigo dyeing method inspired by the indoxyl glucoconjugate present in the plant P.tinctorium. The researchers proposed as an alternative approach a biocatalytic process using recombinant E.coli to access water soluble β-gluco-indoxyl (indican). At the point of coloring, indican gets hydrolyzed by β-glucosidase enzymes forming indoxyl, which suddenly undergoes oxidation to leucoindigo and further crystallizes to indigo giving the typical blue denim its signature character.</p>

<p>A substrate for visualising alkaline phosphatase activity. It produces a purple insoluble end product that is detected visually, when used together with nitroblue tetrazolium. The substrate system is versatile and has functions in a variety of applications, including Northern, Southern, and Western blotting, in situ hybridization, ELISAs, microarray and immunohistochemistry. DMF soluble.</p>

<p>5-Bromo-4-chloro-3-indolyl a-D-xylopyranoside is a chromogenic substrate used to detect the specific enzymatic activity of alpha-xylosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl a-D-xylopyranoside is used in bacterial xylan metabolism studies and plant cell wall degradation assays.</p>

<p>Aldol® 515 propionate is a chromogenic substrate for many enzymes, including beta-galactosidase, β-glucuronidase, α-galactosidase, β-galactosidase, and acid phosphatase. It can be used as a substrate in the detection of staining and enzyme activity in diagnostic testing. Aldol® 515 propionate has been shown to be an effective ligand for the protein Glu-C.</p>

<p>5-Bromo-4-chloro-3-indoxyl-alpha-L-arabinopyranoside is a fluorogenic substrate that can be used in enzyme and chromogenic assays. It is a high purity, stable product with a CAS number of 53673-61-7. This product has been shown to have fluorescence properties under acidic conditions, as well as chemiluminescence properties when exposed to an oxidizing agent. This compound can be used for the detection of bacterial activity in culture media and for the detection of pathogens in environmental samples. The conjugate can also be used for food testing.</p>

<p>Alternative chromogenic substrate for alpha-D-galactosidase</p>

<p>Please enquire for more information about 5-Nitro-3-indoxyl-β-D-galactopyranoside including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>3,3'-Diaminobenzidine (DAB) is an organic compound derived from benzidine. DAB is water-soluble as the tetrahydrochloride salt and is used in immunohistochemical staining of nucleic acids and proteins. Within research in Alzheimer's disease, Aβ protein amyloid plaques are targeted by a primary antibody, and subsequently by a secondary antibody, which is conjugated with a peroxidase enzyme. This will bind DAB as a substrate and oxidize it, producing an easily observable brown colour. 3,3’-Diaminobenzidine tetrahydrochloride hydrate is also available as the tetrahydrochloride salt and as the free base.</p>

<p>Beta-naphthyl stearate is a chromogenic substrate for measuring enzymatic activity.</p>

<p>3-Indoxyl caprylate is a chemiluminescent substrate that is used in the detection of bacteria. It has been shown to be a very sensitive and selective fluorogenic substrate for the detection of bacterial activity, which can be used with an appropriate enzyme reaction. 3-Indoxyl caprylate has also been shown to be a potent quencher of bioluminescence, and can be used as a chromogenic substrate in diagnostic assays. 3-Indoxyl caprylate is often used as a culture media supplement or food additive to enhance its growth properties. It has been found to have high purity and quality levels, making it suitable for use in diagnostics and research.</p>

<p>6-Chloro-3-indoxyl-beta-D-glucuronic acid methyl ester is a chromogenic substrate that can be used for the detection of indoxyl glucuronide in urine and other biological fluids. 6-Chloro-3-indoxyl-beta-D-glucuronic acid methyl ester is a white or light yellow crystalline solid that is soluble in water. It has a melting point of about 80°C and is stable at pH levels ranging from 2 to 12. The CAS number for this compound is 13982-87-6.</p>

<p>4-Nitrophenyl 6-O-(alpha-D-mannopyranosyl)-alpha-D-mannopyranoside is a chromogenic substrate for alpha-mannosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 6-O-(alpha-D-mannopyranosyl)-alpha-D-mannopyranoside is used in different applications such as the Lysosomal storage disorder diagnosis (e.g., alpha-mannosidosis)</p>

<p>5-Bromo-4-chloro-3-indoxyl myristate is a chemiluminescent substrate that can be used in the detection of peroxide. It reacts with hydrogen peroxide to emit light, which can be detected using a luminometer. 5-Bromo-4-chloro-3-indoxyl myristate is also used for the detection of bacteria, such as Staphylococcus aureus and Escherichia coli, by staining. The compound is also used for diagnostic tests to identify diseases such as tuberculosis. 5-Bromo-4-chloro-3 indoxyl myristate is soluble in organic solvents and water, not toxic, and has a high purity level.</p>

<p>Substrate for visualizing alkaline phosphatase activity. Produces a magenta colored insoluble end product that is detected visually, when used together with Nitroblue tetrazolium. The substrate system is versatile and functions in a variety of applications, including Northern, Southern, and Western blotting, in situ hybridization, ELISAs and immunohistochemistry. DMF soluble.</p>

<p>Aldol® 470 L-alanine ester, hydrochloride is a fluorescent chemiluminescent substrate. It is a ligand with high purity and high quality, which has been patented in the European Patent Office (EP 2427431) and the United States Patent and Trademark Office (US 8940909). The product can be used for chemiluminescence, staining, fluorescence, bioluminescence, enzyme substrate and food testing. Aldol® 470 L-alanine ester, hydrochloride is an excellent fluorescent chemiluminescent substrate that can be used for conjugation to enzymes or antibodies.</p>

<p>6-Chloro-1H-indol-3-yl octanoate is a fluorogenic substrate that can be used to detect enzymes such as phosphatases, lipases, esterases, and proteases. 6-Chloro-1H-indol-3-yl octanoate is a ligand for metal ions such as Cu(II), Ni(II) and Zn(II). This product has high purity and quality. It is an enzyme substrate for many enzymes such as phosphatases, lipases, esterases, and proteases. This product can be used in diagnostics of various substances including food testing, environmental testing and staining. 6CIO is a chromogenic substrate that can be used to detect bioluminescence reactions.<br>6CIO is CAS No. 159954-35-5.</p>

<p>Chromogenic substrate for carboxylesterase and lipase. 2-naphthol is released upon hydrolyzation. By simultaneous coupling with a diazonium salt, the corresponding azo-dye is formed. Naphthols can also be detected by fluorescence analysis. Used in an colorimetric assay for the extracellular lipase of Pseudomonas fluorescens B52.</p>

<p>Please enquire for more information about 3-Indoxyl choline phosphate including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>Histochemical substrate for alkaline phosphatase</p>

<p>2-Nitrophenyl-beta-D-thiogalactopyranoside is a chromogenic substrate used to detect the presence of galactosidase enzymes in various biological and biochemical assays. It consists of a nitrophenyl group attached to a galactose sugar molecule through a thioglycosidic bond. When acted upon by enzymes such as galactosidases, it releases the colored nitrophenyl group, which can be detected using spectrophotometry. This substrate is commonly used in assays for the detection of LacZ activity in bacterial cultures, as lacZ encodes for β-galactosidase.</p>

<p>Aldol® 495 phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatase activity. The colorless enzyme substrate is cleaved by acid and alkaline phosphatases secreted by bacteria in liquid or solid media, yielding orange color and green fluorescence. Aldol® 495 phosphate can also be used in Western blots and other immunoassay applications with phosphatase-coupled secondary antibodies. Aldol® 495 phosphate can be used under aerobic and anaerobic conditions.</p>

<p>Aldol® 584 is a fluorogenic substrate that can be used for enzymatic and chemical assays. It is an important component of the Cymit Quimica Aldol Reagent Kit and other diagnostic kits. Aldol® 584 is used in immunoassays, such as ELISA, to measure the activity of serine proteases, including cathepsin D, elastase, and leukocyte elastase. This product has a CAS number of 2484873-11-0.</p>

<p>Aldol 495 acetate is a chromogenic substrate that contains the chemical compound Aldol. It is used in food testing, diagnostics, and environmental testing. Aldol 495 acetate can be used as a ligand to detect the presence of enzymes or bacteria in culture media and diagnostics. This product has been shown to have a high purity and quality.</p>

<p>5-Bromo-3-indolyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a lapis substrate, a chromogenic substrate that yields a deep blue color after cleavage. It is used in lysosomal storage disorder screening such as for Sanfilippo syndrome.</p>

<p>Aldol® 484 beta-D-glucopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of ?-glucosidase activity. The colorless enzyme substrate is cleaved by bacteria producing ?-glucosidase in liquid or solid media, yielding intense red color and green fluorescent signal. Aldol® 484 beta-D-glucopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>Aldol® 515 nonanoate hydrochloride is a chromogenic and fluorogenic enzyme substrate for detection of lipase activity. The colorless product is cleaved by C9 esterases produced by bacteria in liquid or solid media, yielding a red color and red fluorescence when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 515 nonanoate can be used under aerobic and anaerobic conditions. Well detectable color/fluorescence of bacterial colonies or liquid cultures is obtained within 24-48 h. In the presence of a purified lipase, color and fluorescence develops within minutes to few hours.</p>

<p>Chromogenic substrate yielding a soluble chromophore for the colorimetric assay of α-D-galactosidase upon cleavage. Used for the detection of hydrolases in tissues and membranes.</p>

<p>5-Bromo-4-chloro-3-indolyl b-D-galactofuranoside is a chromogenic substrate used to detect the specific enzymatic activity of beta-D-galactofuranosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl b-D-galactofuranoside is used in assays for detecting bacterial pathogens (e.g., Mycobacterium tuberculosis).</p>

<p>Aldol® 518 myo-inositol phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatidylinositol specific phospholipase C (PI-PLC, EC 4.6.1.13) activity. The colorless enzyme substrate is cleaved by PI-PLC positive microorganisms grown in liquid or solid media, yielding intense red color. In combination with Aldol® 355 fluorescence enhancer, red fluorescence can also be obtained. Aldol® 518 myo-inositol phosphate thus enables the detection and identification of various bacteria, such as, pathogenic strains of Listeria, Clostridium, Bacillus, and Staphylococcus species. Aldol® 518 myo-inositol phosphate is suitable for use under aerobic and anaerobic conditions.</p>

<p>Chromogenic substrate for α-glucosidase</p>