Altri

<p>For detection of beta-galactosidase activity. <br>The colors of the chromogen can be adjusted by incorporating different functional groups, e.g. cyano or nitro group, which yields purple or black green colonies with beta-galactosidase positive strain.</p>

<p>Aldol® 518 choline phosphate is a fluorogenic substrate that is used in the detection of enzyme activity. It has been shown to be an effective substrate for enzymes such as beta-galactosidase and phosphatases, and can be used in the detection of bacterial growth. Aldol® 518 choline phosphate is also a chromogenic substrate for the detection of alkaline phosphatase. It has been shown to have high purity and bioluminescence properties, making it useful for detecting bacteria in culture media or environmental testing. The conjugate of Aldol® 518 choline phosphate with various ligands can be used to detect specific proteins or antibodies.</p>

<p>4-Nitrophenyl b-D-cellotetraoside is a chromogenic substrate utilized for the detection and quantification of cellulase enzyme activity. Cellulase enzymes hydrolyze the beta-1,4-glycosidic bonds of cellulose, a major component of plant cell walls. Upon cleavage by cellulase, it releases 4-nitrophenol, a compound that exhibits a yellow color and absorbs strongly at 405 nm, allowing for easy spectrophotometric measurements of cellulase activity levels in various sample types, including bacterial and fungal cultures, environmental samples, and industrial applications.</p>

<p>3-Nitrophenyl alpha-D-galactopyranoside is a chromogenic substrate to measure the activity of the alpha-galactosidase enzymes. Upon hydrolysis by galactosidase, 3NPG releases a yellowish product, 3-nitrophenol, which can be easily measured spectrophotometrically. Some of the applications include enzyme assays, screening for alpha-galactosidase-producing microorganisms, and studying the kinetics and inhibition of alpha-galactosidase enzymes.</p>

<p>For the detection of beta-galactosidase activity</p>

<p>Chromogenic substrate to visualize beta-glucuronidase activity; produces a red color</p>

<p>5-Bromo-6-chloro-3-indoxyl butyrate is an enzyme substrate that is used for the detection of bacteria and fungi in environmental testing, food testing, and diagnostics. It is also used as a fluorogenic substrate in bioluminescence and chemiluminescence assays. 5-Bromo-6-chloro-3-indoxyl butyrate binds to proteins on the cell surface, causing an increase in fluorescence when excited by light of a specific wavelength. The conjugate can be detected using a variety of methods, including staining with dyes such as safranin or methylene blue. This product has high purity and high quality.</p>

<p>4-Nitrophenyl 2,3,4,6-tetra-O-acetyl-b-D-thioglucopyranoside is a fluorescent dye that is used in recording and registration. It has been shown to be a very sensitive reporter of protein interactions in living cells. This dye binds to proteins via the hydroxyl groups on its sugar moiety, and has been shown to bind to a variety of proteins such as myosin light chain kinase and phosphatidylinositol 4-kinase. The fluorescent properties of this dye make it useful for tracking protein localization in the cell.</p>

<p>8-Hydroxyquinoline b-D-glucopyranoside (8-OHG-glu) is a chromogenic enzyme substrate commonly used to detect the presence of beta-glucosidase in biological samples. When hydrolyzed by beta-glucosidase, 8-OHG-glu produces a yellow-orange color, indicating the presence of the enzyme. This substrate is particularly useful for measuring beta-glucosidase activity in soil and plant samples.</p>

<p>Chromogenic β-glucosaminidase substrate yielding a yellow solution upon cleavage, used particularly in yeasts and molds.</p>

<p>Also known as BCIP; can be used for visualization of phosphatase labelled antibodies on Western blots. This method quickly became extremely popular in diagnostics and biology research.</p>

<p>1-Naphthyl b-D-glucuronide sodium salt is a chromogenic substrate commonly used to assay the activity of beta-glucuronidase enzymes. It is hydrolyzed by beta-glucuronidase to yield a yellow-colored product, 1-naphthol, which can be quantified spectrophotometrically. This substrate is widely used in diagnostic tests for detection of beta-glucuronidase activity, particularly in clinical microbiology for the identification of E. coli and other enteric bacteria. With its sodium salt form, it boasts increased solubility in aqueous solutions, ensuring seamless integration into various experimental settings.</p>

<p>Indoxyl substrate targeting the enzyme inositol monophosphatase; Magenta color</p>

<p>Aldol® 455 beta-D-glucopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-glucosidase activity. The colorless enzyme substrate is cleaved by beta-glucosidase producing bacteria grown in liquid or solid media, yielding a yellow color and green to orange fluorescence. Aldol® 455 beta-D-glucopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>4-Nitrophenyl laurate is a chromogenic substrate for lipase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl laurate is used in different applications such as the Lipase activity screening, clinical diagnostics</p>

<p>Aldol® 515 N-glutaryl-L-phenylalanine amide is a chromogenic and fluorogenic enzyme substrate used for detection of L-glutamate-phenylalanine (GluPhe)-specific peptidase activity. The colorless enzyme substrate is cleaved the GluPhe-specific peptidase, yielding development of orange-red coloration. In addition, red fluorescence is generated when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 515 N-glutaryl-L-phenylalanine amide can be used in enzyme assays and live bacterial cultures. Aldol® 515 N-glutaryl-L-phenylalanine amide is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.</p>

<p>Aldol® 484 beta-D-galactopyranoside is a chromogenic enzyme substrate for beta-galactosidase activity. The colorless product is cleaved by lactose-utilizing bacteria in liquid or solid media, yielding intense red color. Aldol® 484 beta-D-galacto-pyranoside can also be used in beta-gal reporter gene assays or for identification of beta-gal positive transformants on agar. Aldol® 484 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>p-Nitrophenylphosphorylcholine is a substrate molecule for enzymes that catalyze phospholipid biosynthesis. It is the most common substrate used to study the enzyme activity of phosphatidylcholine synthase and phospholipase A2 in human serum. The reaction mechanism is not well understood, but it appears to involve autocatalysis and an initial nucleophilic attack by p-nitrophenolate on the carbonyl carbon atom of the choline moiety. p-Nitrophenylphosphorylcholine has been shown to exhibit receptor activity on cells, which may be due to its ability to bind with basic proteins. This compound can also inhibit meiosis in Drosophila melanogaster and increase levels of natriuretic peptides in rats, as well as cause cell lysis and polymerase chain reactions in mammalian cells.</p>

<p>Aldol 458 nonanoate solution is a high quality, enzyme substrate that can be used for a number of applications. It has been shown to be an excellent chromogenic substrate, bioluminescence, ligand and chemiluminescence. Aldol 458 nonanoate solution has also been used for the detection of staining and diagnostics. The compound is a conjugate with an excellent binding affinity to proteins. This product can be used in food testing, culture media, fluorogenic substrate and environmental testing. CAS No. 2484873-15-4</p>

<p>Please enquire for more information about 3,4-Cyclohexenoesculetine-N-acetyl-glucosamine including the price, delivery time and more detailed product information at the technical inquiry form on this page</p>

<p>4-Fluoro-2-nitrophenyl beta-D-galactopyranoside, a fluorinated analog of ONPG, is a chromogenic enzyme substrate specifically used to detect beta-galactosidase in bacterial media. 4-Fluoro-2-nitrophenyl beta-D-galactopyranoside is used in Lactose intolerance screening, bacterial beta-galactosidase detection (E. coli)</p>

<p>Chromogenic substrate for α-glucosidase yielding a salmon-colored precipitate.</p>

<p>Nalpha-CBZ-L-Citrulline-p-nitroanilide is a synthetic chromogenic enzyme substrate used in protease enzymatic stutides. After hydrolysis, p-nitroaniline is released, which produces a yellow color detectable spectrophotometrically at 405 nm.</p>

<p>Chromogenic substrate yielding a soluble chromophore for the colorimetric assay of α-D-galactosidase upon cleavage. Used for the detection of hydrolases in tissues and membranes.</p>

<p>Aldol® 518 acetate is a fluorogenic substrate for the enzyme aldolase. It is used in diagnostic kits for detecting aldolase in blood and other body fluids, and as an indicator of liver function. Aldol® 518 acetate can be used to detect the presence of aldolase in foods and beverages. The compound has also been shown to be an excellent substrate for bioluminescence and chemiluminescence reactions, which are widely used in research laboratories.</p>

<p>Aldol® 495 nonanoate is a chromogenic and fluorogenic enzyme substrate used for detection of lipase activity. The colorless enzyme substrate is cleaved by C9 esterases, yielding development of an orange coloration. In addition, green fluorescence is generated when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 495 nonanoate can be used in enzyme assays and live bacterial cultures. Aldol® 495 nonanoate is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.</p>

<p>Chromogenic substrate yielding a soluble yellow chromophor for the colorimetric assay of α-L-fucosidase, especially in human serum.</p>

<p>2-Nitrophenyl 2-acetamido-2-deoxy-alpha-D-glucopyranoside is a chromogenic substrate used to assay the activity of enzymes that cleave N-acetyl-alpha-D-glucosaminide, such as chitinases and lysozymes. Upon cleavage by the enzyme of interest, the substrate releases a yellow-colored 2-nitrophenol that can be measured spectrophotometrically. This substrate is widely used in the food industry to monitor the activity of lysozyme in cheese, as well as in biomedical research for studying cellular and molecular mechanisms involved in conditions such as inflammation and cancer.</p>

<p>Aldol® 515 caprylate is a chromogenic and fluorogenic enzyme substrate used for detection of lipase activity. The colorless enzyme substrate is cleaved by C8 esterases, yielding development of red coloration. In addition, red fluorescence is generated when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 515 caprylate can be used in enzyme assays and live bacterial cultures. Aldol® 515 caprylate is suitable for use in liquid and solid media, under aerobic as well as anaerobic conditions.</p>

<p>Substrate for horseradish peroxidase</p>

<p>4-Nitrophenyl-N-acetyl-α-D-glucosaminide is a chromogenic substrate for N-acetyl-β-D-glucosaminidase yielding a yellow solution upon cleavage.</p>

<p>6-Chloro-3-indolyl 2-acetamido-2-deoxy-b-D-glucopyranoside is a pink enzyme substrate commonly used in biochemical research and diagnostic applications. This compound is a derivative of indolyl glucopyranoside, which is known for its ability to produce a colored product upon enzymatic hydrolysis. 6-Chloro-3-indolyl 2-acetamido-2-deoxy-b-D-glucopyranoside is particularly useful for studying glycosidases, enzymes that cleave glycosidic bonds in complex carbohydrates. Its pink coloration makes it an ideal choice for colorimetric assays, enabling researchers to monitor enzyme activity in real-time and facilitating the development of new diagnostic tools and therapeutic strategies.</p>

<p>Celluchrome (short name for remazol brilliant blue carboxymethyl cellulose or RBB-CMC) is a chromogenic substrate for cellulases. With a higher sensitivity, RBB-CMC allows for the detection of the enzyme activity even when small amounts are present.</p>

<p>Aldol® 470 beta-D-galactopyranoside is a chromogenic and fluorogenic enzyme substrate used for detection of beta-galactosidase activity. The colorless enzyme substrate is cleaved by lactose-utilizing bacteria grown in liquid or solid media, yielding yellow coloration and green fluorescence. Aldol® 470 beta-D-galactopyranoside can also be used in beta-galactosidase gene reporter assays or immunoassay applications (ELISA). Aldol® 470 beta-D-galactopyranoside can be used under aerobic and anaerobic conditions.</p>

<p>This powerful chromogenic substrate is designed specifically for the assay and detection of a-L-fucosidase enzyme activity. With a unique combination of bromo, chloro, and indolyl moieties, it offers enhanced stability and promotes color development that is unparalleled in clarity and intensity. Ideal for biochemistry, molecular biology, and related life sciences applications, this substrate provides unmatched performance and reliability for all your a-L-fucosidase enzyme research needs.</p>

<p>1-Naphthyl β-D-glucopyranoside can be used as a chromogenic substrate for beta-glucosidases. It can also be used as a fluorogenic substrate to study carbohydrate metabolism and in screening enzyme inhitibitors in pharmacologic research.</p>

<p>2-Chloro-4-nitrophenyl β-D-cellotrioside is a chromogenic substrate use in cellulase activity assays. This enzyme, which is responsible to break down cellulose, hydrolyzes 2-chloro-4-nitrophenyl beta-D-cellotrioside and releases 2-chloro-4-nitrophenol than can be quantified spectrophotometrically. The use of 2-chloro-4-nitrophenyl beta-D-cellotrioside as substrate for enzymatic assays allows detecting bacterial cellulose in urine tract infections in the biofilm state.</p>

<p>6-Nitro-3-indolyl-b-D-galactopyranoside is a chemiluminescent substrate that reacts with horseradish peroxidase to produce light. 6NIGP is used in the diagnostic field as a fluorogenic or chromogenic substrate for enzymes such as alkaline phosphatase, and as a staining agent for cells, tissues, and other biological materials. 6NIGP has been used in the detection of bacteria, fungi, parasites, and viruses. It is also used in food testing as an enzyme substrate. 6NIGP is purified by recrystallization from water or acetone and can be stored at −20°C.</p>

<p>5-Bromo-4-chloro-3-indoxyl-1-acetate is a chromogenic substrate used to detect the specific enzymatic activity of esterase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indoxyl-1-acetate is used in bacterial esterase activity assays (e.g. Pseudomonas spp.).</p>

<p>Aldol® 514 inositol-phosphate is a chromogenic enzyme substrate used for detection of phosphatidylinositol specific phospholipase C (PI-PLC, EC 4.6.1.13). The colorless enzyme substrate is cleaved by PI-PLC positive microorganisms, yielding a red-orange color. The substrate thus enables the detection and identification of various bacteria, such as, pathogenic strains of Listeria, Clostridium, Bacillus, and Staphylococcus. Aldol® 514 inositol-phosphate is suitable for use under aerobic and anaerobic conditions.</p>

<p>Modified naphtol AS substrate used in histochemical and cytochemical staining</p>

<p>Chromogenic substrate for β-D-fucosidase yielding a blue precipitate.</p>

<p>Chitin azure is a chromogenic substrate for chitinases. It is one of the few specific substrates for this enzyme that produces chito-oligosaccharides from chitin.</p>

<p>Calcium 2-naphthylphosphate, which is the calcim salt of 2-naphthylphosphate, is a chromogenic substrate to measure alkaline phosphatase activity. After enzymatic cleavage, the product yielded (2-naphthol) generates a red-colored complex upon reaction with a diazonium salt like Fast Blue RR or Fast Garnet GBC.</p>

<p>Chromogenic substrate for β-D-galactosidase yielding a precipitate. Mostly used in histochemistry.</p>

<p>Chromogenic substrate for carboxylesterase yielding a blue precipitate upon cleavage. It has been proposed for the rapid detection of Branhamella catarrhalis in a strip test because unlike most other members of the family Neisseriaceae, Branhamella catarrhalis produces a butyrateesterase.</p>

<p>N-Acetylmemantine is a drug that inhibits the activity of acetylcholinesterase in the brain. It has been shown to inhibit cancer cell growth and induce apoptosis, as well as inhibit the reaction of hydrolysis of acetonitrile with hydrochloric acid in an organic solvent. N-Acetylmemantine is synthesized by heating ethylene in the presence of hydrochloric acid. The synthesis yields a white solid that consists mainly of N-acetylmethamphetamine. This drug interacts with cellular membranes, which may be due to its ability to react with cholesterol or phospholipids. The interaction leads to changes in membrane permeability, which can result in apoptosis.</p>

<p>The maunfacturing of blue jeans started 150 years ago as workwear, and are found nowadays in almost every wardrobe. Their renowned indigo color tone is truly unique; nevertheless, the high demand for the dye poses serious sustainability and environmental problems connected with its manufacturing process. Recently, a group of scientists presented an alternative indigo dyeing method inspired by the indoxyl glucoconjugate present in the plant P.tinctorium. The researchers proposed as an alternative approach a biocatalytic process using recombinant E.coli to access water soluble β-gluco-indoxyl (indican). At the point of coloring, indican gets hydrolyzed by β-glucosidase enzymes forming indoxyl, which suddenly undergoes oxidation to leucoindigo and further crystallizes to indigo giving the typical blue denim its signature character.</p>

<p>Naphthol AS-BI N-acetyl-beta-D-glucosaminide is a fluorogenic substrate for the pararosaniline-glyoxalase (PG) reaction. This substrate is used to study enzymatic activity in two human isoenzymes of PG, PG I and PG II. The conversion of this substrate by PG I or PG II results in a color change from yellow to red. Naphthol AS-BI N-acetyl-beta-D-glucosaminide may be used as an experimental tool to measure the activity of these enzymes and to study the effect of inhibitors on their activity. It can also be used as a marker for studying enzyme transfer between cells.</p>

<p>A chromogenic substrate for β-galactosidase.</p>