Altri

<p>This product is designed for the sensitive color detection andidentification of various bacteria, such as pathogenic strains of Listeria, Clostridium, Bacillus and Staphylococcus. 5-Bromo-4-chloro-3-indoxyl myo-inositol-1-phosphate (X-phos-inositol; X-IP) is a chromogenic indicator for phosphatidyl-inositol specific phospholipase C(PI-PLC, EC 4.6.1.13). The colorless product is cleaved by PI-PLC positive microorganisms in liquid media or on solid culture media and yields a blue-green color precipitate.</p>

<p>5-Amino-3-indoxyl-beta-D-galactopyranoside (AIG) is a chromogenic substrate that is used for the detection of beta-galactosidase activity. AIG is also used in food testing, diagnostics, and environmental testing. The detection of beta-galactosidase activity can be done by either using the fluorogenic substrate 4-methylumbelliferyl galactopyranoside or the bioluminescence system of firefly luciferase and luciferin. This product has a CAS number of 110114-03-0 and a molecular weight of 417.5 g/mol.</p>

<p>Aldol® 455 alpha-D-glucopyranoside is a high quality, environmentally tested, staining, and ligand for bioluminescent assays. It has been shown to be a chromogenic substrate for diagnostics and a fluorogenic substrate for enzymes such as alpha-glucosidase, producing a yellow/green-orange color. Aldol® 455 alpha-D-glucopyranoside is also used in culture media and food testing.</p>

<p>Chromogenic substrate for aryl-sulfatase. Yields a blue precipitate upon cleavage.</p>

<p>2-Chloro-4-nitrophenyl b-D-lactoside is a chromogenic enzyme substrate used to study the activity of enzymes. It produces a color when hydrolyzed, which makes it a valuable tool in screening for enzyme activity and studying enzyme kinetics.</p>

<p>Chromogenic substrate for carboxylesterase yielding a magenta precipitate upon cleavage. Mainly used in histochemistry.</p>

<p>6-Chloro-3-indoxyl caprylate is a high quality, enzyme substrate, chromogenic substrate, bioluminescence, chemiluminescence ligand, staining and diagnostics reagent. It has CAS No. 159954-35-5 and is used in food testing, culture media and environmental testing. 6-Chloro-3-indoxyl caprylate is also a fluorogenic substrate that is used for high purity applications such as conjugation with other molecules.</p>

<p>p-Nitrophenylphosphorylcholine is a substrate molecule for enzymes that catalyze phospholipid biosynthesis. It is the most common substrate used to study the enzyme activity of phosphatidylcholine synthase and phospholipase A2 in human serum. The reaction mechanism is not well understood, but it appears to involve autocatalysis and an initial nucleophilic attack by p-nitrophenolate on the carbonyl carbon atom of the choline moiety. p-Nitrophenylphosphorylcholine has been shown to exhibit receptor activity on cells, which may be due to its ability to bind with basic proteins. This compound can also inhibit meiosis in Drosophila melanogaster and increase levels of natriuretic peptides in rats, as well as cause cell lysis and polymerase chain reactions in mammalian cells.</p>

<p>4-Nitrophenyl 4,6-benzylidene-alpha-D-glucopyranoside is a chromogenic substrate for alpha-glucosidase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl 4,6-benzylidene-alpha-D-glucopyranoside is used in different applications such as the Pompe disease diagnosis, glycogen metabolism research</p>

<p>5-Bromo-4-chloro-3-indolyl a-D-xylopyranoside is a chromogenic substrate used to detect the specific enzymatic activity of alpha-xylosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl a-D-xylopyranoside is used in bacterial xylan metabolism studies and plant cell wall degradation assays.</p>

<p>5-Bromo-4-chloro-3-indoxyl-alpha-D-N-acetylneuraminic acid, cyclohexylammonium salt is a Fluorogenic substrate used for the detection of an enzyme that converts sialic acid to fluorescein. This product has a high quality and purity with a CAS number. It is used in Culture media as a conjugate, Enzyme substrate, Food testing, High purity, Diagnostics, ligand, Chromogenic substrate, Environmental testing. The product has been tested and found to be safe for use with animals and humans.</p>

<p>Chromogenic substrate for beta-D-glucosidase, yielding a blue precipitate and contained in a chromogenic culture media for the detection of Enterococci, Streptococci, Klebsiella species, Enterobacter species and Listeria species. It is an indolyl carbohydrate that is the beta-D-glucoside of 3-hydroxy-1H-indole, in which the indole moiety is substituted at positions 4 and 5 by chlorine and bromine, respectively. It is used to test for the presence of the enzyme, beta-glucosidase, which cleaves the glycosidic bond to give 5-bromo-4-chloro-3-hydroxy-1H-indole; this immediately dimerises to give an intensely blue product.</p>

<p>2-Nitrophenyl-1-thio-beta-D-galactopyranoside is a nitroaryl compound that can be synthesized from beta-D-galactopyranosides and nitric acid. It has a molecular conformation that is stabilized by hydrogen bonds, which allows it to form supramolecular assemblies with other molecules. The nitro group in this molecule is responsible for the formation of the nitro group in 1,2,4,5,-tetranitrobenzene.</p>

<p>1-Naphthyl beta-D-glucuronide (NPG) is a chromogenic enzyme substrate used to detect the presence of β-glucuronidase activity. Upon hydrolysis by the enzyme, it produces a yellow product that can be detected by spectrophotometry or visually. It is commonly used in clinical assays to detect the presence of the enzyme in urine samples, as elevated levels can indicate liver or urinary tract diseases. It is also utilized in environmental testing, as some bacteria strains can produce the enzyme as a by-product, making NPG a useful tool for detecting these strains in contaminated water or food sources.</p>

<p>3-Indolyl 2-acetamido-2-deoxy-b-D-galactopyranoside is a fluorogenic substrate that reacts with the enzyme β-galactosidase to produce a blue product. This product can be used to diagnose the presence of β-galactosidase in culture media, as well as in food and environmental testing. 3IAG is also a chromogenic substrate that can be used for detection of β-galactosidase activity.</p>

<p>Chromogenic substrate for alpha-L-rhamnosidase</p>

<p>Aldol 495 nonanoate is a bioluminescent substrate that can be used to measure the activity of Aldolase, which is an enzyme that catalyzes the conversion of glyceraldehyde-3-phosphate and dihydroxyacetone phosphate to D-glyceraldehyde. This substrate is used in various diagnostic assays, including food testing and staining. Aldol 495 nonanoate was developed by Cymit Quimica Pharmaceuticals and has been patented by EP 2427431 and US 8940909.</p>

<p>Calcium 2-naphthylphosphate, which is the calcim salt of 2-naphthylphosphate, is a chromogenic substrate to measure alkaline phosphatase activity. After enzymatic cleavage, the product yielded (2-naphthol) generates a red-colored complex upon reaction with a diazonium salt like Fast Blue RR or Fast Garnet GBC.</p>

<p>2-O-(4-Nitrophenyl)-a-D-N-acetylneuraminic acid ammonium salt is a chromogenic substrate commonly used in the detection and quantification of sialidase activity. Sialidases are a family of enzymes that hydrolyze sialic acid residues from glycoconjugates. This substrate is used to detect bacterial and viral sialidases, which play an important role in pathogen infectivity. The ammonium salt form of the substrate is preferred for its solubility in water-based assay systems.</p>

<p>4-Nitrophenyl laurate is a chromogenic substrate for lipase. Upon hydrolysis, para-nitrophenol is released yielding a yellowish colour. 4-Nitrophenyl laurate is used in different applications such as the Lipase activity screening, clinical diagnostics</p>

<p>4-Nitrophenyl palmitate is a lipase-sensitive chromogenic substrate commonly used in the study of lipase and esterase activity. Its hydrolysis by lipases produces a yellow 4-nitrophenol, which can be easily detected spectrophotometrically, offering a quantitative measure of enzyme activity. This substrate is widely utilized in the screening of lipase-producing microorganisms and the optimization of enzyme-catalyzed reactions in various fields, including biotechnology and pharmaceutical research.</p>

<p>Aldol 584 is a chromogenic substrate that is used in enzymatic assays to detect the presence of an enzyme. It is a triethylammonium salt with beta-D-glucuronic acid. Aldol 584 is used in diagnostic procedures and can be used as a substrate for fluorogenic substrates and chemiluminescent substrates. This product has been shown to be effective in detecting the presence of bile in feces, erythrocyte hemolysis, and the presence of proteinases in human urine. It can also be used as a food testing agent or to measure bacterial activity in culture media.</p>

<p>6-Bromo-2-naphthyl β-D-galactopyranoside is a chromogenic substrate commonly used for the detection of the enzymatic activity of β-galactosidase. It can produce a yellow precipitate upon hydrolysis by β-galactosidase, indicating the presence of the enzyme. It is often used in molecular biology applications to detect gene expression or to monitor cloning efficiency.</p>

<p>Chromogenic substrate for α-glucosidase yielding a salmon-colored precipitate.</p>

<p>5-Bromo-4-chloro-3-indolyl N-acetyl-a-D-neuraminic acid ammonium salt is a substrate used to detect and quantify sialidase activity. Sialidases are enzymes that cleave sialic acid residues from glycoconjugates, and their activity is important in various cellular processes. The substrate is commonly used in research aimed at understanding the role of sialidases in diseases such as influenza and cancer.</p>

<p>Aldol 518 Palmitate is a high purity and high quality, bioluminescent substrate for use in diagnostic applications. Aldol 518 Palmitate is an enzyme substrate that is used for the detection of hydrogen peroxide through chemiluminescence or bioluminescence. It can be used in the diagnosis of bacterial infections and to test food products for spoilage. The enzyme substrate has a CAS number of 646-12-2.</p>

<p>Chromogenic substrate for α-mannosidase yielding a salmon colored precipitate upon cleavage.</p>

<p>2-Nitrophenyl beta-D-galactopyranoside-6-phosphate cyclohexylammonium salt is a chromogenic substrate used to detect the enzymatic activity of beta-galactosidase-6-phosphatase. After cleavage, the nitrophenyl moiety is released exhibiting a light yellowish color. 2-Nitrophenyl beta-D-galactopyranoside-6-phosphate cyclohexylammonium salt is used in diagnostic assays such as lysosomal storage disorder.</p>

<p>3-Nitrophenyl beta-D-glucopyranoside is a chromogenic enzyme substrate commonly used to assay for beta-glucosidase activity. Upon hydrolysis by the enzyme, it produces a yellow product that can be easily detected by spectrophotometry. This substrate is often used in the field of carbohydrate chemistry and biochemistry to study enzyme kinetics and inhibition in both in vitro and in vivo systems.</p>

<p>2-Nitrophenyl b-D-fucopyranoside is a chromogenic substrate used in various enzymatic assays to detect the presence of fucosidase enzymes. It consists of a nitrophenyl group attached to a fucose sugar molecule. When acted upon by enzymes such as fucosidases, it releases the colored nitrophenyl group, which can be detected using spectrophotometry.</p>

<p>6-Chloro-3-indoxyl nonanoate is a fluorescent substrate that is used in the detection of beta-galactosidase activity. It has been used to detect the enzyme levels in various culture media and as a high quality, food testing, and environmental testing. The product is also used as a ligand for enzyme inhibition studies. 6-Chloro-3-indoxyl nonanoate has shown to be an excellent fluoroquinolone substrate for chemiluminescence assays. This product is CAS No. 133950-72-8 and is of high purity and high quality.</p>

<p>4-Nitrophenyl 3-O-(a-D-glucopyranosyl)-a-D-glucopyranoside (pNP-Glc-Glc) is a highly sensitive and potent chromogenic substrate for the enzymatic detection and measurement of alpha-glucosidase activity. The rapid color change triggered by the hydrolysis of pNP-Glc-Glc provides a clear and efficient method for monitoring enzyme kinetics, screening inhibitors, and functional characterization of α-glucosidases. This substrate is soluble in aqueous and polar organic solvents and yields a detectable 4-nitrophenol product with a distinct yellow color under both visible and ultraviolet light, enabling quantitative spectrophotometric measurements. With a superior signal-to-noise ratio and maximal sensitivity, pNP-Glc-Glc is a reliable and robust option for biochemical and biomedical research involving glucosidase enzymes.</p>

<p>Chromogenic substrate for α-glucanase</p>

<p>5-Bromo-4-chloro-3-indolyl b-D-galactofuranoside is a chromogenic substrate used to detect the specific enzymatic activity of beta-D-galactofuranosidase. After cleavage, 5-bromo-4-chloro-indoxyl is released, resulting in a blue to blue-green color change in bacterial colonies or media. 5-Bromo-4-chloro-3-indolyl b-D-galactofuranoside is used in assays for detecting bacterial pathogens (e.g., Mycobacterium tuberculosis).</p>

<p>Aldol® 470 choline phosphate is a chromogenic and fluorogenic enzyme substrate used for detection of phosphatidyl-choline specific phospholipase C (PC-PLC, EC 3.1.4.3) activity. The colorless product is cleaved by PC-PLC positive microorganisms in liquid or solid media, yielding a yellow color and green fluorescence. Aldol® 470 choline phosphate therefore, enables the detection and identification of PC-PLC positive pathogenic bacteria, such as, Clostridium perfringens, Pseudomonas aeruginosa, Helicobacter pylori, Legionella pneumophila, Bacillus cereus, Bacillus anthracis, Listeria monocytogenes, and others. Aldol® 470 choline phosphate can be used under aerobic and anaerobic conditions.</p>

<p>Aldol® 484 acetate is a chromogenic substrate for carboxylesterases yielding a red precipitate. In contrast to classical indoxyl substrates, Aldol® substrates can be used under aerobic and anaerobic conditions.</p>

<p>Substrate for visualizing alkaline phosphatase activity. Produces a magenta colored insoluble end product that is detected visually, when used together with Nitroblue tetrazolium. The substrate system is versatile and functions in a variety of applications, including Northern, Southern, and Western blotting, in situ hybridization, ELISAs and immunohistochemistry. DMF soluble.</p>

<p>2-Chloro-4-nitrophenyl alpha-D-mannopyranoside is a substrate used to measure the alpha-mannosidase activity. This enzyme plays a crucial role in the lysosomal degradation of asparagine-linked carbohydrate cores of glycoproteinsis. The use of 2-Chloro-4-nitrophenyl alpha-D-mannopyranoside in diagnostic assays is important as a deficiency in alpha-mannosidase leads to the accumulation of mannose-rich oligosaccharides in lysosomes, causing a range of health problems collectively known as alpha-mannosidosis.</p>

<p>Chromogenic β-galactosidase substrate yielding a yellow solution upon cleavage. Can be used for the detection of coliforms due to their beta-galactosidase activity.</p>

<p>1-Naphthyl β-D-mannopyranoside is a chromogenic (also fluorogenic) substrate. After cleavage, 1-naphthol is released and is used to quantify beta-mannosidase activity.</p>

<p>A chromogenic substrate for β-galactosidase.</p>

<p>5-Bromo-6-chloro-3-indolyl a-D-galactopyranoside is a chromogenic substrate that yields a magenta-colored product after cleavage. The chromophore 5-bromo-6-chloro-indoxyl is released, generating the observable color. It is used in glycolipid metabolism studies.</p>

<p>Chromogenic substrate for α-D-glucosidase yielding a yellow solution upon cleavage. Also used for the detection of glucansucrases and for yeast α-D-glucosidase and for studies of the crystal structure of the complexes of concanavalin A.</p>

<p>4-(Hydroxymethyl)phenyl-beta-D-glucopyranoside tetraacetate is a drug that has been shown to have antidiabetic effects in diabetic patients. It has been shown to inhibit tyrosinase activity and increase the production of gamma-aminobutyric acid (GABA) in brain cells. This compound also has a protective effect against glutamate toxicity, and is being studied as a potential treatment for epilepsy. 4-(Hydroxymethyl)phenyl-beta-D-glucopyranoside tetraacetate inhibits the production of tyrosinase, which is an enzyme responsible for the conversion of tyrosine to melanin. This process can be inhibited by inhibiting tyrosinase activity with 4-(hydroxymethyl)phenyl-beta-D-glucopyranoside tetraacetate.</p>

<p>6-Chloro-3-indoxyl-3-acetate is a chromogenic substrate for carboxylesterase yielding a salmon colored precipitate upon cleavage. Mainly used in histochemistry.</p>

<p>4-Nitrophenyl-beta-L-fucopyranoside is a high-quality pNP enzyme substrate developed for the sensitive detection and quantification of Beta-L-fucosidase activity. The enzymatic hydrolysis of the substrate generates 4-nitrophenol, a yellow chromogenic component that can be conveniently measured spectrophotometrically, allowing for accurate determination of enzyme concentration and kinetics. This substrate finds applications in various research fields, diagnostics, and industrial enzyme production.</p>

<p>Chromogenic substrate targeting beta-galactosidase</p>

<p>For the detection of beta-galactosidase activity</p>

<p>Chromogenic substrate targeting beta- galactosidase and whose color is dependent on the metal chelating.Alizarin-2-beta-D-galactopyranoside is a chromogenic substrate for enzymes based on alizarin and has been reported for the detection of bacterial beta-galactosidase by researchers at the University of Northumbria and the Freeman Hospital, Newcastle upon Tyne in the UK (James, 2000).</p>

<p>Aldol® 515 acetate is a chromogenic and fluorogenic substrate, used for detection of esterase activity. The colorless product is cleaved by purified esterase, or esterase secreted by microorganisms in liquid or solid media, yielding a red color and red fluorescence signal when Aldol® 355 fluorescence enhancer or a suitable matrix is present. Aldol® 515 acetate can be used under aerobic and anaerobic conditions. Well detectable color/fluorescence of bacterial colonies or liquid cultures is obtained within 16-48 h. In the presence of a purified esterase, color and fluorescence develops within minutes to few hours.</p>