Peptidi

Derived from fibronectin, integrin-binding cell adhesive peptide can be used as an adhesion ligand, it contains the cell binding motif RGD. Providing integrin-mediated cell adhesion is a valuable tool for a wide variety of research applications particularly in cell culture and implants. The integrin-binding cell adhesive peptide can also functionalise biomolecules. This has been successfully demonstrated by the generation of a protease-degradable hydrogel containing the integrin-binding cell adhesive peptide that can support skin formation in vitro. Integrin-binding cell adhesive peptide has also been used to form hydrogels to adhere numerous cell lines, for example to study extracellular vesicle secretion.

Peptide H-ERDISTEIYQAGNKP-OH is a Research Peptide with significant interest within the field academic and medical research. This peptide is available for purchase at Cymit Quimica in multiple sizes and with a specification of your choice.

The pathology of cardiovascular disease (CVD) is linked to the health of endothelial cells in the heart.- However, the specific characteristics of the cardiovascular endothelial cells are still being uncovered. The heart homing peptide specifically binds to a receptor on cardiovascular endothelial cells. This peptide can be used as a conjugate to deliver molecules specifically to the heart. This can be a crucial tool in therapeutic drug delivery for CVD, angiogenesis, and thrombosis.

α-connexin carboxyl terminal peptide that specifically targets and maintains Cx43 at gap junction sites between cell-cell membrane borders of breast cancer cell.- Thus it augments gap junction activity and impairs proliferation and survival of breast cancer cells with no effect on non-transformed cells.

Delivery of peptide nucleic acid (PNA) oligonucleotides to targeted cells is becoming a viable therapeutic strategy by redirecting RNA splicing for conditions like Duchenne muscular dystrophy (DMD). The use of novel cell-penetrating peptides (CPP) has helped create targeted, and increased activity of the PNAs delivered.PNA internalization peptides (Pip)  is a specifically designed CPP for conjugation to PNAs. Pips were designed to improve PNA activity observed in HeLa cells determined by splicing redirection assays and increased serum stability determined by mass spectrometry. Of the Pip series, Pip1 enters the cell in an energy-dependent manner, primarily via clathrin-dependent endocytosis. Pip1 conjugated to a PNA was determined to be well transported into the cell but is not the most stable against proteolysis. However, this may benefit some cargo depending on the intracellular destination. As a CPP for DMD, the proteolysis of Pip1 was deemed an issue but showed promise for other conjugates in HeLa cells.Pip1 is labelled at the N-terminus with an alkyne attachment for ease of reaction with an opposite Click reactive partner (azide). Azide-alkyne cycloaddition has become the most popular Click reaction. Alkyne-Pip1 allows various applications, particularly for protein conjugation, modification, and drug delivery.

Histone H3 (1-18) is derived from Histone 3 (H3) which is one of the four core histones (H2A, H2B, H3 and H4) fundamental in compacting eukaryotic DNA into the nucleosome. The nucleosome arises when 147 base pairs of DNA wrap around a H3-H4 tetramer and two H2A-H2B dimers, forming the histone octamer core. Both H4 and H3 are highly conserved and perform roles in binding to segments of DNA which enter and leave the nucleosome and in chromatin formation. Similar to the other core histone, H3 has a globular domain and a flexible N-terminal domain 'histone tail' which can undergo modifications such as acetylation, methylation, phosphorylation and ubiquitination. Due to histones containing a large number of lysine and arginine residues they have a positive net charge which interacts in an electrostatic manner with the negatively charged phosphate groups in DNA. The transcriptional activation or silencing of the chromatin is controlled by ATP-dependent chromatin remodelling factors and histone modifying enzymes which target histone proteins. Both processes function to alter the positioning of the nucleosome, allowing the DNA it to be either available or inaccessible to the transcription machinery.